Glutathione Peroxidase (GPX) - Product Review 23

Protector against oxidative stress

Glutathione peroxidases (GPXs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization.

The classical cytosolic /mitochondrial GPX1 (cGPX) is a seleniumdependent enzyme, first of the GPX family to be discovered. GPX2, also known as gastrointestinal GPX (GI-GPX), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract (1). Extracellular plasma GPX (pGPX or GPX3) is mainly expressed by the kidney from where it is released into the blood circulation (2). Phospholipid hydroperoxide GPX4 (PH-GPX) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine (3). All mammalian GPX family members, except for the recently described Cys containing GPX3 and epididymis-specific secretory GPX (eGPX or GPX5) isoforms, possess selenocysteine at the active site (4-5).

Background References

  1. Takebe, G.. et al. (2002) J. Biol. Chem. 277, 41254-41258.
  2. Avissar, N. et al. (1994) Am. J. Physiol. 267, E68-76.
  3. Bao, Y. et al. (1997) FEBS Lett. 410, 210-212.
  4. Chambers, I. et al. (1986) EMBO J. 5, 1221-1227.
  5. Perry, A. et al. (1992) Biochem. J. 285, 863-870.


Fig. 1: Western Bot Analysis of HL60 cells using the polyclonal -GPX4 antibody LF-PA0055 (Lane 1: Lysat; Lane 2: Immunprecipitate).

Fig. 1: Western Bot Analysis of HL60 cells using the polyclonal -GPX4 antibody LF-PA0055 (Lane 1: Lysat; Lane 2: Immunprecipitate).

 


In the table below all our antibodies for detection of GPX isoenzymes are listed. All Glutathione Peroxidase products: GPX1 antibody, GPX2 antibody, GPX3 antibody, GPX4 antibody, GPX5 antibody



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glutathione Peroxidase (GPX) antibody

Glutathione Peroxidase  (GPX) Rabbit Serum Arabidopsis WB
0.1 ml / €390.00
  AgriSera AB

Glutathione peroxidase 1 / GPX1 antibody

Glutathione peroxidase 1 / GPX1 Sheep IgG AP Bov, Hu E
1 ml / €410.00
  Acris Antibodies GmbH

Glutathione peroxidase 1 / GPX1 antibody

Glutathione peroxidase 1 / GPX1 Sheep IgG Purified Bov, Hu E
1 ml / €400.00
  Acris Antibodies GmbH

Glutathione peroxidase 1 / GPX1 antibody

Western Blot: GPX1 Antibody [NB100-40805] - NB100-40805 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Glutathione peroxidase 1 / GPX1 antibody

Glutathione peroxidase 1 / GPX1 Sheep IgG AP Bov E
0.5 ml / €800.00
  Novus Biologicals Inc.

Glutathione peroxidase 1 / GPX1 antibody

Glutathione peroxidase 1 / GPX1 Rabbit Serum Hu, Ms, Rt C, P, WB
0.2 ml / €400.00
  Novus Biologicals Inc.

Glutathione peroxidase 2 / GPX2 antibody

Western Blot: GPX2 Antibody [NB100-1888] - NB100-1888 staining (1ug/ml) of Human Prostate lysate (RIPA buffer, 30ug total protein per lane).Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Glutathione peroxidase 2 / GPX2 antibody

Western Blot: Glutathione Peroxidase 2 Antibody [NBP1-30950] - Western blot analysis of Glutathione Peroxidase 2 Antibody on MOLT4, diluted at 1:100 with 15% SDS PAGE gel. Rabbit IgG Hu Dot, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Glutathione peroxidase 2 / GPX2 antibody

Western Blot:  Glutathione Peroxidase 2 Antibody[NBP1-32002]-  (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 12% SDS PAGE GTX100292 diluted at 1:1000 Rabbit IgG Hu, Ms P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Glutathione peroxidase 3 / GPX3 antibody

Human Lung Tissue; Rabbit Anti-GPX3 Antibody . Catalog Number: ARP41491_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue . Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells . Primary Antibody Concentration: 1:100 . Other Working Concentrations: 1/600 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-GPX3 Antibody (ARP41491_P050) ) in Human Lung Tissue using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Glutathione peroxidase 3 / GPX3 antibody

Western Blot: GPX3 Antibody [NB100-57858] - NB100-57858 (1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Glutathione peroxidase 3 / GPX3 antibody

Glutathione peroxidase 3 / GPX3 Rabbit Serum Hu, Mky, Rt ICC/IF, WB
25 µl / €160.00
  Novus Biologicals Inc.

Glutathione peroxidase 3 / GPX3 antibody

Glutathione peroxidase 3 / GPX3 Sheep Serum Hu, Ms, Rt C, P, WB
0.15 ml / €400.00
  Novus Biologicals Inc.

Glutathione peroxidase 6 / GPX6 antibody

Glutathione peroxidase 6 / GPX6 Mouse IgG Purified Hu E, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Glutathione peroxidase 7 / GPX7 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG 2704 Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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