Magnetofection by OZ Bioscience - Product Review 19

OZ Biosciences

New Transfection Technology

This method associates DNA with magnetic nanoparticles coated with cationic molecules. The resulting molecular complexes are then transported into cells supported by an appropriate magnetic field.

Magnetofection exploits magnetic force exerted upon gene vectors towards, possibly even into, the target cells. In this manner, the complete applied vector dose gets concentrated on the cells within a few minutes so that 100% of the cells get in contact with a significant vector dose. Inspired by the validated and recognized magnetic drug targeting technology, this innovative method is a revolution for transfection and infection.

Simplicity & Quickness

Efficiency

Significantly increases transfection efficiency, even with very low doses of DNA or vectors, by concentrating the genetic materials on the cells.

Transfection PolyMagIncrease transfection CombiMag
Transfection PolyMagIncrease transfection CombiMag

Standard
Transfection

PolyMag
Magnetofection

Reagent F alone

CombiMag + Reagent F

Confluent Primary Human KeratinocytesConfluent Primary Human Keratinocytes
Gene Silencing SilenceMagInfection ViroMag

Control

SilenceMag
10nM siRNA

Without magnetic
field

With magnetic
field

GFP Stably transfected HeLa

NIH 3T3 Cells lacking CAR receptor infected with
an Adenovirus (coding for LacZ +/- ViroMag)

Universal

Significantly increases transfection efficiency, even with very low doses of DNA or vectors, by concentrating the geneticmaterials on the cells.

Primary Cells

Primary Cells

Chondrocytes (Photo)
Peripherical Blood Lymphocytes Aortic Endothelial Cells Human Fibroblasts Nasal & Pulmonary Epithelium...

Cell Lines

Cell Lines

293
MCF-7
HeLa
Hep G2
CHO-K1 ...

 

  List of cells

Versatile

OZ Biosciences offers three types of ready-to-use reagents:

   PolyMag, designed for all nucleic acids

   SilenceMag, specific to siRNA delivery

   CombiMag, unique solution for all vectors: viruses & transfection reagents

   ViroMag, unique solution for viral applications

Comfort for both cells & scientists

Non Toxic

PolyMag versus Electroporation

Do not electroshock your cells anymore!

Economic

  No expensive equipment required

  Save materials : very low doses of DNA are sufficient

  Save money : less reagents are used

  Exceptional quality / price ratio

Economic with materials and time

Reagents and Kits

Which reagent / kit is convenient for my application?
Use this table to choose the appropriate kit to your needs.

Nucleic acids

Cell Lines

Primary cells

Hard to Transfect

Convenient Starting Kit
(Pricing*)

DNA

PolyMag
or
CombiMag
associated to DreamFect or your usual transfection reagent

KC30296

RNAs

ODN

Virus

ViroMag

KC30596

siRNA

SilenceMag

KC30396
or
KC30496

shRNA

dsRNA

*** Click the desired starting kit catalog number for pricing information and datasheet download

Starting Kits

If this is your first try with the Magnetofection technology, we recommend our starting kits to begin with. Please note that magnetic plate is required to create an appropriated magnetic field.

Cat. Number

Product
(Pricing-Information***)

Volume
(µL)

Number of transfections/ µg

Number of transfections in 24-well plate*

Number of transfections in 96-well plate*

KC30296

Starting Kit 1

2x100

2x100

2x100

2x1000

KC30396

siRNA Starting Kit 2

200

na

200 4

>4004

KC30496

Super Starting Kit 3

2x100+200

2x100

2x100+200 4

2x100+400 4

KC30596

ViroMag Starting Kit 5

200µL

-

20-200 **

60-1000 **

* See Instruction Manual for more details regarding number of transfections in various culture supports
** Number of transductions
*** Click the desired product for pricing information and datasheet download
1 Contain 100µL of PolyMag, 100µL of CombiMag and the Magnetic Plate
2 Contain 200µL of SilenceMag and the Magnetic Plate
3 Contain 100µL of PolyMag, 100µL of CombiMag, 200µL of SilenceMag and the Magnetic Plate
4 SilenceMag: Number of transfection given for a concentration of 10 nM siRNA
5 Contain 200µL of ViroMag and the Magnetic Plate

 

Selection Kits

Cat. Number

Product
(Pricing-Information***)

Volume
(µL)

Number of transfections/ µg

Number of transfections in 24-well plate*

Number of transfections in 96-well plate*

MF10096

Magnetic Plate

-

-

-

-

KM30200

Selection Kit 5

2x100

2x100

2x100

2x1.000

KM30300

Selection Kit SiP 6

200+100

100

200 9+100

400 9+1000

KM30350

Selection Kit SiC 7

200+100

100

200 9+100

400 9+1000

KM30400

Super Selection Kit 8

2x100+200

2x100

2x100+200 9

2x100+400 9

* See Instruction Manual for more details regarding number of transfections in various culture supports
** Click the desired product for pricing information and datasheet download
5 Contain 100µL of PolyMag and 100µL of CombiMag
6 Contain 200µL of SilenceMag and 100µL of PolyMag
7 Contain 200µL of SilenceMag and 100µL of CombiMag
8 Contain 100µL of PolyMag, 100µL of CombiMag, 200µL of SilenceMag
9 SilenceMag: Number of transfection given for a concentration of 10 nM siRNA

 

Reagents

Cat. Number

Product
(Pricing-Information***)

Volume
(µL)

Number of transfections/ µg

Number of transfections in 24-well plate*

Number of transfections in 96-well plate*

MF10096

Magnetic Plate

-

-

-

-

PN30100

PolyMag 100

100

100

100

1000

PN30200

PolyMag 200

200

200

200

2000

PN31000

PolyMag 1000

1000

1000

1000

10000

CM20100

CombiMag 100

100

100

100

1000

CM20200

CombiMag 200

200

200

200

2000

CM21000

CombiMag 1000

1000

1000

1000

10000

SM10200

SilenceMag 200 10

200

-

200

400

SM10500

SilenceMag 500 10

500

-

500

1000

SM11000

SilenceMag 1000 10

1.000

-

1000

2000

SM13000

SilenceMag 3000 10

3x1000

-

3000

6000

VM40100

ViroMag 100

100 µL

-

10-100**

30-500**

VM40200

ViroMag 200

200 µL

-

20-200**

60-1000**

VM41000

ViroMag 1000

1000 µL

-

100-1000**

300-5000**

* See Instruction Manual for more details regarding number of transfections in various culture supports
** Number of transductions
*** Click the desired product for pricing information and datasheet download
10 Number of transfection given for a concentration of 10 nM siRNA.

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