R1253F Mouse IgG (H+L chain) antibody

Search for all "Mouse IgG"

2 mg / €200.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Mouse IgG

Properties: (H+L chain)
Presentation: FITC
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay.

Properties for Mouse IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Mouse Immunoglobulin G
Presentation FITC
Applications E, F, ICC/IF
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Mouse IgG whole molecule.
Application Suitable for ELISA, Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
Flow Cytometry: 1/2,000-1/10,000.
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Koshkin V, Ailles LE, Liu G, Krylov SN. Metabolic suppression of a drug resistant subpopulation in cancer spheroid cells. J Cell Biochem. 2015 Jun 6. doi: 10.1002/jcb.25247. PubMed PMID: 26054050.
General Readings
  1. The and Feltkamp, Immunology 18; 865, 1970. (Conjugation)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Fluorescein isothiocyanate (Molecular Weight 390 daltons).
Conjugation Chemistry: Isothiocyanate
Absorption emission:
495 nm / 528 nm
Molar radio:
3.1 moles FITC per mole of Rabbit IgG
Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/ml BSA (Ig and Protease free)
Lyophilized purified IgG fraction
Restore with 1.0 ml of deionized water (or equivalent).
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
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