R1458C5 Rabbit IgG (H+L chain, adsorbed) antibody

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1 mg / €300.00

Product Description for Rabbit IgG

Properties: (H+L chain, adsorbed)
Presentation: Cy5

Properties for Rabbit IgG

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Rabbit Immunoglobulin G
Presentation Cy5
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Rabbit IgG, whole molecule.
Add. information Conjugation References: Modified from GE Healthcare Bio-Sciences Corp., Piscataway, NJ.
Application FLISA: 1/10,000-1/50,000
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots, add glycerol to 50%) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
(MW 975 daltons)
Absorption emission:
650 nm / 667 nm
Molar radio:
12.9 moles Cy5 per mole of Goat IgG (Acceptable Range: 6-20)
Immunoaffinity Chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
Lyophilized purified Ig fraction
Restore with 1.0 ml of deionized water (or equivalent).
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
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