AgriSera AB

  1. Quick-Filters:
  2. Cited (243)
  3. Highly Validated (2)

Items 1 to 20 of 617 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

6xHistidine Epitope Tag (HHHHHH) antibody

500 femtomoles of His-tagged proteins IsiA, NifH, PsbA, PsbB and PetC were loaded per gel well in Agrisera PEB extraction buffer.  Proteins were separated on  4-12 % NuPAGE PAGE Bis-Tris polycacrylamide gel (Invitrogen) and blotted 1h to PVDF. Blots were blocked with   for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat, anti-mouse IgG horse radish peroxidase conjugated, from Agrisera AS11 1772) diluted to 1:25 000 in 2 % ECL Advance blocking reagent for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was  5 seconds.
Apparent molecular weight of recombinant proteins: IsiA - 27 kda, NifH - 34 kDa, PsbA - 30-37 kDa, PsbB - 40 kDa, PetC - 23 kDa. Mouse IgG2b HIS.H8 / EH158 Purified Dot, E, IP, WB
50 µg / €300.00
  AgriSera AB

14-3-3 GRF antibody

14-3-3 GRF Rabbit Serum algae Neochloris oloabundans, Arabidopsis, Arabidopsis thaliana, Lilium longiflorum, Mesembryanthemum crystallinum. WB
50 µl / €300.00
  AgriSera AB

14-3-3 protein c-isoform antibody

14-3-3 protein c-isoform Rabbit Serum Horderum vulgare, Pl E, WB
0.1 ml / €390.00
  AgriSera AB

40S ribosomal protein S14-1 antibody

5 µg of total protein from Arabidopsis thaliana (1), Zea mays (2), Chlamydomonas reinhardtii (3), Salmo salar (4)  extracted with PEB  were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 2 minutes. Rabbit Serum Arabidopsis, Chlamydomonas reinhardtii, Zea mays WB
0.1 ml / €410.00
  AgriSera AB

A12.2 antibody

A12.2 Rabbit Aff - Purified Arabidopsis WB
0.2 mg / €420.00
  AgriSera AB

AADA1 antibody

AADA1 Rabbit Aff - Purified Chlamydomonas reinhardtii, Nicotiana tabacum WB
0.2 mg / €390.00
  AgriSera AB

ABI2 antibody

ABI2 Rabbit Aff - Purified Arabidopsis WB
50 µg / €300.00
  AgriSera AB

Abscisic acid (ABA) antibody

Abscisic acid (ABA) Rabbit Ig Fraction Euphorbia pulcherrima E, ICC/IF
0.1 ml / €520.00
  AgriSera AB

Abscisic acid (ABA) antibody

Abscisic acid (ABA) Rabbit Ig Fraction Pl E, R
1 mg / €300.00
  AgriSera AB

Abscisic acid (ABA) antibody

Abscisic acid (ABA) Rabbit Aff - Purified Pl
0.2 mg / €300.00
  AgriSera AB

Abscisic Acid Insensitive 5 / ABI5 antibody

2, 5 and 10 ug of total protein (run on separate lanes) extracted from the Columbia ecotype (Arabidopsis thaliana) seeds using Acetone extraction was separated using the Bolt® Bis-Tris Plus Gel system on a 4-12% gradient SDS-PAGE gel, blotted using the turbo-blot system (BIO-RAD) to transfer onto a PVDF membrane (7min). SNAP-ID (Millipore) system was used for blocking and antibody labeling. Blocking occured for 30 minutes (no agitation, 0.05 % skim milk in dest. water). Primary antibody labeling was done for 10 minutes at 1:200 dilution. Followed by 3x10 ml washes (PBST). Then blotted with secondary antibody (anti-rabbit IgG HRP conjugated from Agrisera AS09 602) for 10 min (1:1000 dilution) followed by three washes.Blot was developed using ECL as per manufacturer’s instructions. Exposure time was 2 min.  Courtesy of Nay Chi, Pogson Lab, Australia Rabbit Aff - Purified Arabidopsis, Arabidopsis thaliana WB
50 µg / €300.00
  AgriSera AB

ACC Synthase 7 antibody

10 ug of total protein extract from Nicotiana benthamiana leaves expressed ACS7­His­GST (1), 5 ug of purified ACS7­His­GST from N. benthamiana ACS7­His­GFP fusion protein transiently expressed in N. benthamiana leaves was purified on Ni ‐ NTA resin(2) and (3). Samples were separated on 10% SDS­PAGE and transfered to PVDP membrane by semi­dry blotting (1h). Blot was blocked with 3% skim milk in PBS­T (0,01% Tween 20) for 1h in RT with agitation. Then blot was incubated with primary antibody anti ACS7 at dilution of 1:500 in PBS­T for 1h in RT with agitation. After washes (3 times for 5 min. in PBS­T) blot was incubated with secondary antibody (anti­rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) at dilution 1:25000 for 1h in RT. The blot was washed 5 times for 5 min. with PBS­T and developed for 2 min with ECL­Plus according to the manufacturer's instructions. Exposure time was 3 min.

Courtesy of Dr. Agata Cieśla, Ludwików's lab, UAM, Poland Rabbit Aff - Purified Arabidopsis WB
0.1 mg / €300.00
  AgriSera AB

ACO1 (+ACO2/3) antibody

ACO1 Rabbit Serum ACO2, ACO3 ), Arabidopsis thaliana (ACO1, Solanum lycopersicum WB
0.1 ml / €390.00
  AgriSera AB

Acrylamide antibody

Acrylamide Chicken IgY Purified AP
1 ml / €410.00
  AgriSera AB

Actin antibody

Actin cytoskeleton in 5 days old Arabidopsis thaliana seedlings. Actin signal shown in green, PIN1 in red and DAPI in blue. The material has been fixed in 2 % formaldehyde for 45 minutes. Tissue cleaning has been performed before immunolocalization. Rabbit anti-actin primary antibody was diluted in 1:250 and anti-rabbit Alexa 488 and Alexa 555 were both diluted in 1: 500 (Invitrogen). Scale bar - 20 µm.
Courtesy: Dr. Taras Pasternak Rabbit Serum Arabidopsis, Hordeum vulgare, Zea mays ICC/IF, WB
50 µl / €300.00
  AgriSera AB

ADGP antibody

ADGP Rabbit Serum Arabidopsis, Horderum vulgare, Zea mays WB
50 µl / €340.00
  AgriSera AB

Aflatoxin M1 antibody

Aflatoxin M1 Mouse Lyophilized E
50 µg / €300.00
  AgriSera AB

AGL / GDE antibody

AGL / GDE Rabbit IgG Aff - Purified Can, Hu, Ms, Rb, Rt IP, WB
0.2 mg / €420.00
  AgriSera AB

AGO9 antibody

80 µg of Arabidopsis thaliana soluble total cell extract (extracted in 20 mMTris pH7.5, 5mM MgCl2, 2.5mM DTT, 300mM NaCl, 0.1% NP-40, 1% proteaseinhibitor) was separated on 6% SDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 5% low-fat milk powder in TBS-TT (0.25% TWEEN20; 0.1% Triton-X) and probed with anti-AGO9 antibody (1:10 000, 1h) and secondary anti-rabbit antibody (HRP conjugated, Agrisera AS09 602) (1:15 000, 1 h) in TBS-TT containing 5% low fat milk powder. Antibody incubations were followed by washings in TBS-TT. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-PLUS detection reagent according the manufacturer's instructions (GE Healthcare). Exposure time was 30 seconds. Courtesy Dr. Ericka Havecker, University of Cambridge. Rabbit Aff - Purified Arabidopsis IP, WB
0.1 mg / €420.00
  AgriSera AB

AHB2 / Hemoglobin 2 antibody

AHB2 expression in stage 1-15 flowers Indicated protein amounts of Arabidopsis thaliana extracts are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds. Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA   Rabbit Serum Arabidopsis WB
50 µl / €300.00
  AgriSera AB

Items 1 to 20 of 617 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn