Antibody BCN

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BLM (1218-1233) antibody

The BCN antibody recognizes BLM. Whole cell BLM 160 kDa l 4), extracts (30 μg) from wt cells and from BLM patientderived cells where fractionated in a 6% SDS-PAGE, transferred to nitrocellulose membrane, and probed with the anti-BLM BCN antibody diluted 1:100. Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. Rabbit IgG Aff - Purified Hu WB
50 µl / €300.00
  Antibody BCN

DAGLA (C-term) antibody

Immunohistochemical detection of DAGLα using BCN4788 on formaldehyde fixed rat cerebellum slices Rabbit Serum Hu, Ms, Rt C, ICC/IF
0.1 ml / €350.00
  Antibody BCN

DAGLA (C-term) antibody

Immunoblot of DAGL-α by using antibody (BCN4789) at 1/100 in 40 µg of rat brain membranes, developed using the ECL technique. Rabbit IgG Aff - Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €400.00
  Antibody BCN

DAGLB antibody

Immunohistochemical detection of DAGL-β using BCN4790 on formaldehyde fixed rat cerebellum slices Rabbit Serum Hu, Ms, Rt C
0.1 ml / €350.00
  Antibody BCN

DAGLB antibody

Immunoblot of DAGL-β by using antibody (BCN4791) at 1/100 in 40 µg of rat brain membranes, developed using the ECL technique. Rabbit IgG Aff - Purified Hu, Ms, Rt C, WB
0.1 ml / €400.00
  Antibody BCN

E2F7 (Isoform 2 specific) antibody

The anti-E2F7-A BCN4774 antibody is able to supershift a DNA-protein complex that contains E2F7-A. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from mocktransfected Saos-2 cells (-) or nuclear extracts from Saos-2 cells transfected with pcDNA3-HAE2F7- A (+) were analyzed by EMSA. Extracts were preincubated in the absence (None) or in the presence of an antibody to the HA tag or the anti-E2F7-A BCN4774 antibody. The arrow indicates the position of the HA-E2F7-Acontaining complex, whereas arrowheads indicate the position of supershifted complexes. Rabbit Serum Hu EMSA, IP, WB
0.1 ml / €360.00
  Antibody BCN

E2F7 (Isoform 1 specific) antibody

The anti-E2F7-B BCN4775 antibody is able to supershift a DNA-protein complex that contains E2F7-B. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from Saos-2 cells transfected with pcDNA3-HAE2F7- B (+) were analyzed by EMSA. Extracts were preincubated in the absence (None) or in the presence of an antibody to the HA tag,anti-E2F7-B BCN4775 antibody, or the corresponding pre-immune serum (PI). The arrow indicates the position of the HA-E2F7-B-containing complex, whereas arrowheads indicate the position of super-shifted complexes. Rabbit Serum Hu EMSA, IP, WB
0.1 ml / €360.00
  Antibody BCN

E2F7 (Isoform 1+2) antibody

The anti-E2F7 BCN4776 antibody is able to supershift DNA-protein complexes that contain either E2F7-A or E2F7-B. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from Saos-2 cells transfected with either pcDNA3-HA-E2F7-A (A) or pcDNA3-HA-E2F7-B (B) were analyzed by EMSA. Extracts were preincubated in the presence of an antibody to the HA tag, the anti-E2F7-B (BCN4775), the anti-E2F7 BCN4776 antibody, or the corresponding pre-immune serum (PI). The arrow indicates the position of the HA-E2F7-A- or HA-E2F7-B-containing complexes, whereas arrowheads indicate the position of super-shifted complexes. Rabbit Serum Hu EMSA, IP, WB
0.1 ml / €360.00
  Antibody BCN

Endonuclease G (C-term) antibody

The western blot signal is reduced in extracts from cultured cells with EndoG gene silencing driven by shRNA interference. The antibody also detects the overexpressed protein in cell cultures. The quality of our antibody has been compared to several commercially available EndoG antibodies. Other commercial antibodies only detect a band at around 35 kDa in Western Blot. EndoG has a molecular weight of 32.6 kDa (immature) and 27,7 KDa (mature, processed at the mitochondria). We detected EndoG overexpression (OvEx) in rat primary cultured cells. We also checked endogenous EndoG expression in cells transduced with control lentivirus (scrambled, scr) and lentiviral vectors inducing specific EndoG silencing by short hairpin RNA interference (RNAi). Other commercial antibodies are reported to detect endogenous EndoG as a band of 35 kDa. However, in our hands these antibodies only detected overexpressed EndoG and an endogenous unspecific band at an apparent molecular weight bigger than EndoG. On the contrary, our antibody correctly detected both repression of endogenous EndoG silencing and EndoG overexpression. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 ml / €300.00
  Antibody BCN

FAIM (Long form) (3-18) antibody

Immunofluorescence localization (arrows) of FAIML proteins in cortical layer V at P5 (3). After blocking, sections were incubated overnight with BCN 4777 (1:600), and was visualized using secondary Alexa Fluorconjugated antibodies. Sections were counterstained with Bisbenzimide, mounted onto slides, and viewed under confocal microscopy. Rabbit Serum Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €250.00
  Antibody BCN

FAIM (Short form) antibody

The Western Blot signal is reduced in extracts from cells with FAIMS gene silencing driven by shRNA interference. PC12 cells infected with a lentivirus carrying a vector for FAIMS shRNAi. Rabbit Serum Hu, Ms, Rt WB
0.1 ml / €300.00
  Antibody BCN

FANCB (C-term) antibody

The BCN4783 antibody recognizes FANCB, and its decrease in the presence of siRNA. Whole cell extracts (30 μg) from Hela cells with or without siRNA against FANCB where fractionated in a 6% SDSPAGE, transferred to nitrocellulose membrane, and probed with the anti-FANCB BCN4783 antibody diluted 1:500 (Panel A) and with anti-vinculin as loading control (Panel B). Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. Rabbit IgG Aff - Purified Hu WB
50 µl / €250.00
  Antibody BCN

FANCL (C-term) antibody

The BCN antibody recognizes FANCL in IF. HeLa cells were fixed in 4% formaldehyde in PBS, permeabilized with 0.5% Triton x100 in PBS and incubated overnight at 4ºC with anti-FANCL BCN diluted 1:500 in 3% BSA in PBS. Goat anti Rabbit Alexa Fluor 488 was used as a secondary antibody at a dilution of 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, WB
50 µl / €250.00
  Antibody BCN

MRE11 (249-264) antibody

The BCN antibody recognizes MRE11A, and its decrease in the presence of siRNA. Whole cell extracts (30 μg) from HEK-293 cells with or without siRNA where fractionated in a 8% SDS-PAGE, transferred to nitrocellulose membrane, and probed with the anti-WRN BCN antibody diluted 1:100. Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. Rabbit IgG Aff - Purified Hu WB
50 µl / €320.00
  Antibody BCN

NAPE-PLD antibody

Immunohistochemical detection of NAPE-PLD using BCN4792 on formaldehyde fixed rat cerebellum slices. Rabbit Serum Hu, Ms, Rt C
0.1 ml / €350.00
  Antibody BCN

NAPE-PLD antibody

Immunoblot of NAPE-PLD by using antibody (BCN4793) at 1/100 in 40 µg of rat brain membranes, developed using the ECL technique. Rabbit IgG Aff - Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €400.00
  Antibody BCN

Nibrin (119-134) antibody

The BCN antibody recognizes NBS1 and its mutated form. Whole cell extracts (30 μg) from wt cells and from NBS patient-derived cells where fractionated in a 8% SDS-PAGE, transferred to nitrocellulose membrane, and probed with the anti- NBS1 BCN antibody diluted 1:100. Peroxidaseconjugated anti-rabbit antibodies followed by ECL were then used. Rabbit IgG Aff - Purified Hu WB
50 µl / €340.00
  Antibody BCN

PALB2 (759-774) antibody

The BCN antibody recognizes FANCN, and its decrease in the presence of siRNA. Whole cell extracts (30 μg) from Hela cells with or without siRNA where fractionated in a 6% SDS-PAGE, transferred to nitrocellulose membrane, and probed with the anti- FANCN BCN antibody diluted 1:500 (Panel A) and with anti-vinculin as loading control (Panel B). Peroxidaseconjugated anti-rabbit antibodies followed by ECL were then used. Rabbit IgG Aff - Purified Hu WB
50 µl / €300.00
  Antibody BCN

SERPINC1 / Antithrombin-III (Citrullinated) antibody

Western blot analysis against Citrullinated Antithrombin. Human antithrombin purified from plasma, non-citrullinated (ATn) or citrullinated (Atc) with PAD (Peptidyl arginil deiminasa from Sigma). ECL revealing method was used. Mouse IgG1 3B6B4ii Supernatant Hu E, WB
50 µl / €500.00
  Antibody BCN

WRN (223-237) antibody

The BCN antibody recognizes WRN, and its decrease in the presence of siRNA. Whole cell extracts (30 μg) from HEK-293 cells with or without siRNA where fractionated in a 6% SDS-PAGE, transferred to nitrocellulose membrane, and probed with the anti-WRN BCN antibody diluted 1:100. Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. Rabbit IgG Aff - Purified Hu WB
50 µl / €280.00
  Antibody BCN

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