6xHistidine Epitope Tag

Principal name

6xHistidine Epitope Tag antibody

Available reactivities

Mam (Mammalia), All Species, Hu (Human)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of 6xHistidine Epitope Tag antibody

In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins [1]. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells [1,2]. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available.

General readings

Bayer & Wilchek, Methods in Enzymology, 184; 138-160, 1990.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (SM1693P/PX) visualised with Rabbit anti Mouse IgG -HRP conjugated (SP1012HRP). Mouse IgG1 AD1.1.10 Purified C, E, IP, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS tagged protein probed with mouse anti Histidine Tag - Biotin (SM1693B) and visualised with Streptavidin - HRP Mouse IgG1 AD1.1.10 Biotin C, E, IP, WB
0.1 mg / €610.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (SM1693P/PX) visualised with Rabbit anti Mouse IgG -HRP conjugated (SP1012HRP). Mouse IgG1 AD1.1.10 Purified C, E, IP, WB
2 mg / €1,250.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Immunofluorescence of His-tagged fusion protein expressed in 293 cells. Left Lane: Untransfected control; Right Lane: Transfected cells. Mouse IgG2b BV-G020 Aff - Purified ICC/IF, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. Western blot of His tagged protein (vector pET 30C) using AM05288PU-N antibody. Lane 1: Whole cellular extract of BL21 (DE3) pJT4pTT9 bacteria carrying gene encoding for His-Rel E protein (uninduced). Lane 2: Induced. Lane 3: Induced (1/2 dilution). Lane 4: Purified His-Rel E protein (~ 17 kDa). Mouse IgG2a IPA2C5.1 Purified WB
0.1 mg / €360.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS monoclonal antibody (6AT18). Mouse IgG1 6AT18 Ascites WB
0.4 ml / €370.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot (AP) of several His-tagged proteins  from supernatants (A) and after purification (B) by affinity chromatography using metal chelate columns (Ni2+, Co2+) as matrix. The anti-His-tag antibody concentration used was 0.1µg/ml. Rabbit Aff - Purified WB
50 µg / €210.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. The blot was blocked with 3% BSA in TBST for 45 min at RT. Antibody was incubated with blot at a 1/1,000 dilution in TBST with 3% BSA for 1 hour at RT. Detection occurred using HRP Goat-anti-Rabbit IgG  diluted 1/80,000 in blocking buffer for 30 min at RT. Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate which has the His epitope tag incorporated through a C-terminal linkage (~42 kDa). Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~40 kDa). A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. Proteins were transferred to nitrocellulose using standard methods. Molecular weights were estimated by comparison to standards (lane M). Rabbit Aff - Purified C, E, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Biotin C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit HRP C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody (SM1693F). Mouse IgG1 AD1.1.10 FITC F, ICC/IF
0.1 mg / €440.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot analysis of His-Ubiquitin recombinant protein probed with MOUSE ANTI HISTIDINE TAG:HRP (SM1693HRP). Mouse IgG1 AD1.1.10 HRP E, WB
0.1 mg / €580.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag antibody Cat.-No SM1693P Mouse IgG1 AD1.1.10 Purified C, E, IP, WB
1 mg / €610.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Mouse IgG 6A9-E2 Hu WB
0.1 mg / €410.00
  Abnova Taiwan Corp.

6xHistidine Epitope Tag (HHHHHH) antibody

Immunoblot analysis of cell lysates. Lane 1 : His/Bcl2. Lane 2 : His/Erk1. Lane 3 : His/MEK3. Lane 4 : His/Cdc2. Mouse IgG2b 6A7 E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

6xHistidine Epitope Tag (HHHHHH) antibody

1 : His-tagged SARS-CoV spike protein gp24.
2 : His-tagged SARS-CoV spike protein gp120. Mouse IgG1 HT84 E, F, ICC/IF, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Mouse IgG2a IPA2C5.1 WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Mouse IgG1 AD1.1.10 WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Mouse IgG1 His17 E, IP, WB
0.2 mg / €400.00
  Abnova Taiwan Corp.

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot analysis of polyhistidine tagged recombinant HIS at 0.1 µg ( A ) and 0.01 µg ( B ) . Rabbit IgG Purified WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

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