6xHistidine Epitope Tag

Principal name

6xHistidine Epitope Tag antibody

Selected pictures

6xHistidine Epitope Tag antibody - AM00195PU-N
AM00195PU-N - Western blot analysis of His in 293 cells transfectedwith His-tagged vector (Lane 2) and Control vector (Lane 1).
6xHistidine Epitope Tag antibody - AM00195PU-N
AM00195PU-N - Immunofluorescence of His-tagged fusion protein expressed in 293 cells. Left Lane: Untransfected control; Right Lane: Transfected cells.

Available reactivities

Mam (Mammalia), All Species, Hu (Human)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of 6xHistidine Epitope Tag antibody

In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins [1]. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells [1,2]. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available.

General readings

1. Conrath, K. et al. (2001) Camel single binding-domain antibodies as modular binding units in bispecific and bivalent antibody constructs.
J. Biol.Chem. 276 (10): 7346 - 7350
2. Bahi, A. et al. (2004) CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus.
Eur. J. Neurosci. 19:1621-1633.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

6xHistidine Epitope Tag (HHHHHH) antibody

Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody (SM1693F). Mouse IgG1 AD1.1.10 FITC F, ICC/IF
0.1 mg / €440.00
  Acris Antibodies GmbH

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