Beta-galactosidase tag

Principal name

Beta-galactosidase tag antibody

Gene ID

945006 (lacZ)

Available reactivities

E. coli (Escherichia coli), Aspergillus, Bac (Bacteria), All Species

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of Beta-galactosidase tag antibody

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

General readings

This product has been used in:Peterson KR et al. Transgenic Cre expression mice for generation of erythroid-specific gene alterations. Genesis 39:1-9 (2004). PubMed PMID: 15124222
General / background references:The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18:865-73 (1970). PubMed PMID: 5310665

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-galactosidase tag (Center) antibody

Western blot of purified E. coli  beta-galactosidase sample; reduced sample, immunostained by mAb BG-02 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG1 BG-02 Purified E. coli ICC/IF, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Figure 1. Western blotting using Fluorescein conjugated anti-Beta-galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were  resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to Nitrocellulose and blocking using blocking buffer for fluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase (Cat.-No. R1064F) diluted to 1/10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System. Other detection systems will yield similar results. Rabbit Serum E. coli C, E, P, WB
2 ml / €440.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit C, WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 2E9 Purified Aspergillus, E. coli E
1 mg / €310.00
  HyTest Ltd.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 DC1 4C7 Bac E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 DC3 10D6 Purified All Species E
0.1 mg / €340.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 2E9 Bac E
0.2 mg / €250.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Gal present in a partially purified preparation (arrowhead). Approximately 1µg of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye 800 fluorescence image was captured using the Infrared Imaging System. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG FITC Bac E, ICC/IF, WB
1 mg / €290.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit IgG HRP Bac E, P, WB
1 mg / €310.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 BG-02 Aff - Purified Bac ICC/IF, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Beta-galactosidase tag

Beta-galactosidase tag E. coli 600 U/mg
5 mg / €380.00
  Acris Antibodies GmbH

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