DYKDDDDK Epitope Tag

Principal name

DYKDDDDK Epitope Tag antibody

Available reactivities

All Species, Mam (Mammalia), Hu (Human)

Available hosts

Rabbit, Mouse, Chicken, Goat, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F)

Background of DYKDDDDK Epitope Tag antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK.

General readings

Chubet, R.G. and Brizzard, B.L. (1996) Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques 20(1):136-141.
Slootstra, J.W. et al., (1997) Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5. Mol Divers 2(3):156-164.
Robeva, A.S. et al., (1996) Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure. J Biochem Pharmacol 51:4, 545-55.
Fulton, J.E. et al., (1995) Functional analysis of avian class I (BFIV) glycoproteins by epitope tagging and mutagenesis in vitro. Eur J Immunol. 25(7): 2069-2076.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DYKDDDDK Epitope Tag antibody

Figure 2. Monoclonal Antibody to detect FLAG conjugated proteins. Twenty-four (24) clones were randomly selected and grown up from gylcerol stocks by inoculating 0.5 mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatograophy and eluted in 40 µL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG™ antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY. Mouse IgG2a 29E4.G7.H11 Purified E, F, IP, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

Figure 2. Monoclonal Antibody to detect FLAG conjugated proteins. Twenty-four (24) clones were randomly selected and grown up from gylcerol stocks by inoculating 0.5 mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatograophy and eluted in 40 µL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG™ antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY. Mouse IgG2a 29E4.G7.H11 Purified E, F, IP, WB
25 µl / €190.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Rabbit Purified E, WB
50 µg / €420.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Rabbit IgG Purified E, WB
0.1 mg / €530.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

Figure 1. Western blot analysis of extract from 293 cells transfected with Flag-tagged Bad using Flag-Tag Polyclonal Antibody AP08111PU. Rabbit Aff - Purified All, Mam WB
0.1 ml / €260.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

Figure 1. Western blot analysis of extract from 293 cells transfected with Flag-tagged Bad using Flag-Tag Polyclonal Antibody AP08111PU. Rabbit Aff - Purified All, Mam WB
50 µl / €210.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

Figure 1. Western blot analysis of a DYKDDDDK-tagged recombinant protein (bacterial lysate) using the DYKDDDDK Tag Antibody (Cat. #AP11019PU-N). Rabbit Ig Purified WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

Western blot using the antibody at a dilution of 1:2,500 to detect 1.0 μg of recombinant protein containing the DYKDDDDK epitope tag. This antibody will detect both amino and carboxy terminal linked DYKDDDDK  recombinant proteins. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 30 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Aff - Purified C, E, WB
0.25 mg / €270.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Rabbit Biotin C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Rabbit HRP C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

Figure 1C: Merged figures A and B - confirmation of nuclear localization of the fusion protein (cell nuclei stained with DAPI (blue). Mouse IgG1 F-tag-01 Purified All Species ICC/IF, IP, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

DYKDDDDK Epitope Tag antibody

FLAG epitope tag monoclonal antibody, clone 6F4.E4 ( Cat # MAB2094 ) to detect FLAGTM conjugated proteins detects both C terminal linked and N terminal linked FLAGTM tagged recombinant proteins by western blot. Mouse IgG 6F4.E4 E, ICC/IF, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Rabbit IgG Purified E, WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

DYKDDDDK Epitope Tag antibody

Western blot analysis of extract from 293 cells transfected with Flag-tagged Bad using Flag-Tag polyclonal antibody ( Cat # PAB7705 ). Rabbit IgG WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Rabbit IgG Hu C, E, ICC/IF, IP, WB
0.25 mg / €390.00
  Abnova Taiwan Corp.

DYKDDDDK Epitope Tag antibody

The whole cell lysate from the over-expressed FLAG tagged recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with FLAG-tag polyclonal antibody ( Cat # PAB12767 ) at 1 : 1000. Lane 2 was pre-incubated by immunizing peptide. Rabbit IgG E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Rabbit IgG E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Rabbit IgG Aff - Purified E, WB
0.1 mg / €640.00
  Novus Biologicals Inc.

DYKDDDDK Epitope Tag antibody

1B - cell nuclei stained with DAPI (blue) Mouse IgG1 F-tag-01 Purified ICC/IF, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

DYKDDDDK Epitope Tag antibody

DYKDDDDK Epitope Tag Chicken Aff - Purified E, ICC/IF, IP, WB
0.1 mg / €320.00
  Novus Biologicals Inc.

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