HA Epitope Tag (YPYDVPDYA)

Principal name

HA Epitope Tag (YPYDVPDYA) antibody

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Chicken, Goat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of HA Epitope Tag (YPYDVPDYA) antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

General readings

(Conjugation) Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HA Epitope Tag (YPYDVPDYA) antibody

Figure 1. Western blot analysis of extracts from 293 cells transfected with HA-tagged Akt using HA-Tag Polyclonal Antibody AP08109PU. Rabbit Aff - Purified All WB
0.1 ml / €260.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Mouse IgG2b 2C16 Biotin ICC/IF, IP, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Mouse IgG2b 2C16 FITC ICC/IF
0.1 mg / €410.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Mouse IgG2b 12CA5 Purified ICC/IF, IP, WB
0.2 mg / €420.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

Figure 1. Typical Western blot (10% SDS-PAGE) of HA-tagged Rit, a Ras-familiy GTPase, expressed in HEK293 cells and immunoprecipitated with anti HA-agarose (AM05263AG-N) antibody (Lane 1), lysate of HA-tagged Rit expressed in HEK293 cells (Lane 2) and control HEK293 cells (Lane 3) probed with clone 14G7 anti-Rit. Mouse IgG2b 12CA5 Agarose IP
1 ml / €370.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Mouse IgG2b 12CA5 Purified E, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

Immunocytochemistry/Immuno-fluorescence using HA tag antibody: Immunofluorescence staining of HA Tag fusion protein (transcription factor) in a stable expressing cell line (B) and control cell line (A). Mouse IgG3 HA.C5 Purified Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

Figure 1. HA-tagged recombinant protein was expressed in COS7 cells. 15 µg of cell lysate was loaded onto a gel to test the HA tag antibody (AM11008SU-N). Mouse IgG2b 12CA5 Ascites WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit IgG Purified E, P, WB
50 µg / €420.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit IgG Purified E, P, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

Anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by western blot. Polyclonal rabbit-anti-HA epitope tag at a 1:10,000 dilution was used to detect 1.0 µg of recombinant transcription
factor protein containing the HA epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:4000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit IgG Biotin E, IP, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit IgG HRP E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

Immunohistochemistry: Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on Formalin Fixed Paraffin Embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 µm were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4°C overnight followed by signal detection using Horseradish Peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Rabbit IgG Aff - Purified E, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

figure 1. Western blot analysis of anti-HA tag Pab (Cat. #AP11018PU-N)in HA-tagged recombinant protein bacterial lysate. Rabbit Ig Purified WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Mouse IgG2b 12CA5 E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

HA Epitope Tag (YPYDVPDYA) antibody

Western blot analysis on 293 cells that were immunoprecipitated with HA-tag monoclonal antibody, clone 12CA5 (Agarose) ( Cat # MAB1912 ) (lane 1), lysate of HA-tagged Rit expressed in 293 cells (lane 2), and control 293 cells (lane 3) probed with Rit monoclonal antibody, clone 14GG7. Mouse IgG2b 12CA5 IP, WB
1 ml / €400.00
  Abnova Taiwan Corp.

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit IgG Purified WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

HA Epitope Tag (YPYDVPDYA) antibody

Western blot analysis of extract from 293 cells, using HA-Tag polyclonal antibody ( Cat # PAB7703 ). Rabbit IgG WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

HA Epitope Tag (YPYDVPDYA) antibody

HA-tag polyclonal antibody ( Cat # PAB10343 ) detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue.
Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta.
Sections of 4 µm were prepared from representative paraffin blocks.
HA-tag polyclonal antibody ( Cat # PAB10343 ) was diluted at 1 : 2,000.
Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada. Rabbit IgG Hu E, ICC/IF, IP, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit IgG E, E(capture), E(detection), ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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