Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Principal name

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Available reactivities

Vi (Virus), Chk (Chicken), Hu (Human)

Available hosts

Goat, Rabbit, Mouse, Chicken

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Radioimmunoassay (R)

Background of Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.

General readings

This product has been used in:Randall RE et al. Isolation and characterization of monoclonal antibodies to simian virus 5 and their use in revealing antigenic differences between human, canine and simian isolates. J Gen Virol 68 ( Pt 11):2769-80 (1987). PubMed PMID: 2445904

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western blot using V5 tag antibody [V5.E3]: Western blot analysis of 293 cells transfected with V5-tagged vector (Lane 2) and untransfected control (Lane 1). Mouse IgG1 Purified E, ICC/IF, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Anti-V5 EPITOPE TAG polyclonal antibody detects V5-tagged recombinant protein by western blot. This antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full-length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS-PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar
results. Rabbit IgG Aff - Purified E, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Figure 1. Western blot analysis of anti-V5 Tag Antibody (Cat.#AP11020PU-N) in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab. Rabbit Ig Purified WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

V5 tagged protein detected with Mouse anti V5-Tag (SM1691) Mouse IgG2a SV5-PK1 Purified C, E, F, ICC/IF, IP, R, WB
1 mg / €620.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse IgG2a SV5-PK1 Biotin C, E, WB
0.1 mg / €510.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse IgG2a SV5-PK1 FITC ICC/IF
0.1 mg / €500.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse IgG2a SV5-Pk1 HRP E, WB
0.1 mg / €630.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

V5 tagged protein detected with Mouse anti V5-Tag (SM1691) Mouse IgG2a SV5-PK1 Purified C, E, F, ICC/IF, IP, R, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

V5 tagged protein detected with Mouse anti V5-tag (Cat.-No SM1691) Mouse IgG2a SV5-Pk1 Purified C, E, IP, R, WB
2 mg / €1,250.00
  Acris Antibodies GmbH

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Rabbit IgG Purified WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Rabbit Chk, Hu C, E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western Blot: V5-TAG Antibody [NB100-62264] - V5 tagged protein detected with Mouse anti V5-tag Mouse IgG2a SV5-Pk1 Aff - Purified Vi C, E, F, ICC/IF, IP, P, R, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western Blot: V5 Epitope Tag Antibody (SV5-Pk1) [Biotin] [NB100-64791]- V5 tagged protein detected with Mouse anti V5-tag Mouse IgG2a SV5-Pk1 Biotin C, E, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Chicken Aff - Purified E, ICC/IF, IP, WB
0.1 mg / €320.00
  Novus Biologicals Inc.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Goat Aff - Purified E, ICC/IF, IP, WB
0.1 mg / €320.00
  Novus Biologicals Inc.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western Blot: V5 Antibody [NB600-381] - Western blot analysis of V5-tagged Nicastrin, using NB600-381. Samples: NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2).  NB600-381 was used at 0.2 mcg/ml (1:5000). Rabbit Aff - Purified E, ICC/IF, IP, WB
0.1 ml / €320.00
  Novus Biologicals Inc.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Goat HRP E, ICC/IF, WB
0.1 mg / €380.00
  Novus Biologicals Inc.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Goat Agarose IP
0.1 mg / €380.00
  Novus Biologicals Inc.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Rabbit HRP E, ICC/IF, WB
0.1 mg / €380.00
  Novus Biologicals Inc.

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse IgG2a SV5-PK1 FITC Vi ICC/IF
0.1 mg / €420.00
  Novus Biologicals Inc.

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