GFP

Principal name

GFP antibody

Gene ID

3020 (H3F3A)

Ncbi ID

6100, NP_002098

Available reactivities

All Species, A. victoria, Hu (Human), Hydra

Available hosts

Rabbit, Chicken, Goat, Mouse, Sheep

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), ChIP assay (ChIP), In Situ Hybridization (ISH), Immunoelectron Microscopy (EM), Dot blot (Dot), Flow Cytometry (F)

Background of GFP antibody

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm).
GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.
GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.

General readings

Schoch KG et al. A subset of mouse tracheal epithelial basal cells generates large colonies in vitro. Am J Physiol Lung Cell Mol Physiol 286:L631-42 (2004). PubMed PMID: 12959927
Karabay A. et al Axonal growth is sensitive to the levels of katanin, a protein that severs microtubules. The Journal of Neuroscience 24:5778-5788 (2004).Gongidi V et al. SPARC-like 1 Regulates the Terminal Phase of Radial Glia-Guided Migration in the Cerebral Cortex. Neuron 41:57-69 (2004). PubMed PMID: 14715135
Fischer AC et al. Successful transgene expression with serial doses of aerosolized rAAV2 vectors in rhesus macaques. Mol Ther 8:918-26 (2003). PubMed PMID: 14664794
Wong JM et al. Subnuclear shuttling of human telomerase induced by transformation and DNA damage. Nat Cell Biol 4:731-6 (2002). PubMed PMID: 12198499
Zhang QX et al. Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of action. Biochem J 345 Pt 2:181-4 (2000). PubMed PMID: 10620492
Mesaeli N et al. Calreticulin is essential for cardiac development. J Cell Biol 144:857-68 (1999). PubMed PMID: 10085286
McCabe JB & Berthiaume LG Functional roles for fatty acylated amino-terminal domains in subcellular localization. Mol Biol Cell 10:3771-86 (1999). PubMed PMID: 10564270
Jasinska R et al. Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters. Biochem J 340 ( Pt 3):677-86 (1999). PubMed PMID: 10359651
1. Reinhardt, R. L., Hong, S., Kang, S.-J., Wang, Z.-e., and Locksley, R. M. (2006) Visualization of IL-12/23p40 In Vivo Reveals Immunostimulatory Dendritic Cell Migrants that Promote Th1 Differentiation, 177, 1618-1627.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GFP antibody

Figure 1. Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP; bottom). The natural fluorescence of the produced proteins is shown in the green channel (left), the anti-GFP antibody signal was detected in the red channel (right). The system was carefully tested for overlap of these two optical channels and images were scanned separately in sequential scanning mode. The blue nuclear stain is also shown. Rabbit Purified All Species ICC/IF, IP, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

GFP antibody

Lineage tracing of EGFP expressing migrating pectoral girdle myogenic precursors in the chicken embryos via electroporation using TOL-2-GFP plasmid system. Vibratome sections stained with anti-GFP antibody Cat.-No. TP401 show the labeled muscle fiber soft hepectoral girdle in chicken embryos at stage HH27.
Data was kindly provided by: Nargis Khalida, Rizwan Rehimi: Institute of Anatomy, Dept. of Molecular Embryology, Ruhr University Bochum, Germany Rabbit Purified A. victoria E, IP, WB
0.2 mg / €290.00
  Torrey Pines Biolabs

GFP antibody

Formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag Antibody (Cat.-No AM11009PU-N ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter. The staining shows localization of GFP at the bottom of the hair follicle. The antigen was retrieved in citrate pH 6 in water bath for 25 mins. Mouse IgG1 168AT1211 Purified ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GFP antibody

Western blot analysis of anti-GFP Tag Antibody Cat.-No AM11010SU-N (Clone CA071114E) using purified GFP, YFP and BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab. Mouse IgG 168AT1211.269.64 Ascites WB
0.1 ml / €370.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of GFP protein detected with polyclonal anti-GFP antibody. Wild type GFP (0.1 μg) was used to spike 30 μg of a HeLa whole cell lysate. This antibody detects a 27 kDa band corresponding to the epitope tag GFP. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed overnight at 4° C with the primary antibody diluted in 5% BLOTTO to 1:1,000, followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified A. victoria C, E, ICC/IF, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

GFP antibody

GFP Goat AP A. victoria C, E, WB
1 mg / €620.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Polyclonal anti-GFP antibody at a 1/1,000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. Fluorochrome conjugated anti-Goat secondary antibody was used for detection at 1/300. Panel A shows a lateral view (ventral left). Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up.
Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO. Goat Biotin A. victoria C, E, ICC/IF, WB
1 mg / €510.00
  Acris Antibodies GmbH

GFP antibody

GFP Goat FITC A. victoria C, E, ICC/IF, WB
1 mg / €510.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Goat HRP C, E, WB
1 mg / €510.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Immunofluorescence Microscopy of Anti-GFP (GOAT) Antibody: Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1/500 dilution. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody at 1/10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain. Goat Aff - Purified C, E, ICC/IF, WB
1 mg / €490.00
  Acris Antibodies GmbH

GFP antibody

GFP Goat TRITC E, ICC/IF, WB
1 mg / €510.00
  Acris Antibodies GmbH

GFP antibody

GFP Goat Texas Red C, ICC/IF
1 mg / €510.00
  Acris Antibodies GmbH

GFP antibody

Western Blot of Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP (Mouse) Monoclonal Antibody Peroxidase Conjugate (Cat.-No R1461HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. Mouse IgG2a 9F9.F9 HRP A. victoria C, E, WB
1 mg / €720.00
  Acris Antibodies GmbH

GFP antibody

GFP Mouse IgG F56-BA1.2.3 Purified WB
0.1 mg / €360.00
  Acris Antibodies GmbH

GFP antibody

GFP Mouse IgG1 264-449-2 Purified C, IP, WB
0.1 mg / €520.00
  Acris Antibodies GmbH

GFP antibody

GFP Mouse IgG1 N/K Purified C, E, P, WB
0.1 ml / €610.00
  Acris Antibodies GmbH

GFP antibody

Western blot: The recombinant protein GFP (20, 40 and 80 ng) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP antibody (1/1000). Proteins were visualized using a goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: GFP 20 ng 
Lane 2: GFP 40 ng 
Lane 3: GFP 80 ng Mouse IgG2a AT2G5 Purified E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

GFP antibody

Western blot: The recombinant protein GFP (20, 40 and 80 ng) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP antibody (1/1000). Proteins were visualized using a goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: GFP 20 ng 
Lane 2: GFP 40 ng 
Lane 3: GFP 80 ng Mouse IgG2a AT2G5 Purified E, WB
50 µl / €230.00
  Acris Antibodies GmbH

GFP antibody

GFP Chicken IgG Purified C, IP, WB
0.25 mg / €590.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Rabbit IgG Biotin A. victoria C, E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

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