GFP

Principal name

GFP antibody

Gene ID

3020 (H3F3A)

Ncbi ID

6100, NP_002098

Available reactivities

All Species, A. victoria, Hu (Human), Hydra

Available hosts

Rabbit, Chicken, Goat, Mouse, Sheep

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), ChIP assay (ChIP), In Situ Hybridization (ISH), Immunoelectron Microscopy (EM), Dot blot (Dot), Flow Cytometry (F)

Background of GFP antibody

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm).
GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.
GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.

General readings

Schoch KG et al. A subset of mouse tracheal epithelial basal cells generates large colonies in vitro. Am J Physiol Lung Cell Mol Physiol 286:L631-42 (2004). PubMed PMID: 12959927
Karabay A. et al Axonal growth is sensitive to the levels of katanin, a protein that severs microtubules. The Journal of Neuroscience 24:5778-5788 (2004).Gongidi V et al. SPARC-like 1 Regulates the Terminal Phase of Radial Glia-Guided Migration in the Cerebral Cortex. Neuron 41:57-69 (2004). PubMed PMID: 14715135
Fischer AC et al. Successful transgene expression with serial doses of aerosolized rAAV2 vectors in rhesus macaques. Mol Ther 8:918-26 (2003). PubMed PMID: 14664794
Wong JM et al. Subnuclear shuttling of human telomerase induced by transformation and DNA damage. Nat Cell Biol 4:731-6 (2002). PubMed PMID: 12198499
Zhang QX et al. Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of action. Biochem J 345 Pt 2:181-4 (2000). PubMed PMID: 10620492
Mesaeli N et al. Calreticulin is essential for cardiac development. J Cell Biol 144:857-68 (1999). PubMed PMID: 10085286
McCabe JB & Berthiaume LG Functional roles for fatty acylated amino-terminal domains in subcellular localization. Mol Biol Cell 10:3771-86 (1999). PubMed PMID: 10564270
Jasinska R et al. Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters. Biochem J 340 ( Pt 3):677-86 (1999). PubMed PMID: 10359651
1. Reinhardt, R. L., Hong, S., Kang, S.-J., Wang, Z.-e., and Locksley, R. M. (2006) Visualization of IL-12/23p40 In Vivo Reveals Immunostimulatory Dendritic Cell Migrants that Promote Th1 Differentiation, 177, 1618-1627.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Rabbit IgG HRP A. victoria C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Goat HRP C, E, WB
1 mg / €510.00
  Acris Antibodies GmbH

GFP antibody

Western Blot of Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP (Mouse) Monoclonal Antibody Peroxidase Conjugate (Cat.-No R1461HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. Mouse IgG2a 9F9.F9 HRP A. victoria C, E, WB
1 mg / €680.00
  Acris Antibodies GmbH

GFP antibody

Western Blot of Anti-GFP Antibody Peroxidase Conjugate (GTX26663). 
Lane 1: 50ng of GFP. 
Lane 2: none. 
Primary antibody: none. Secondary antibody: Anti-GFP Antibody Peroxidase Conjugate secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none Goat IgG HRP E, IP, WB
0.1 mg / €320.00
  GeneTex Inc.

GFP antibody

GFP Rabbit HRP E, P, WB
50 µg / €380.00
  Novus Biologicals Inc.

GFP antibody

GFP Rabbit HRP All Species E, WB
50 µl / €270.00
  Novus Biologicals Inc.

GFP antibody

GFP Rabbit HRP All Species E, P, WB
0.1 mg / €390.00
  Novus Biologicals Inc.

7 Item(s)

per page
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