GST-Tag

Principal name

GST-Tag antibody

Gene ID

2950 (GSTP1)

Available reactivities

Sj (Schistosoma japonicum), E. coli (Escherichia coli), Mam (Mammalia), Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F), Dot blot (Dot), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of GST-Tag antibody

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. Recombinant GST was expressed in E.coli and purified by conventional chromatography techniques.

General readings

This product has been used in:
Lehti K et al. Oligomerization through hemopexin and cytoplasmic domains regulates the activity and turnover of membrane-type 1 matrix metalloproteinase. J Biol Chem 277:8440-8 (2002).
Chen, P-L., Chen, C-F., Chen, Y., Xiao, J., Sharp, Z.D. and Lee, W-H. (1998) The BRC repeats in BRCA2 are Critical for RAD51 Binding and Resistance to Methyl Methanesulfonate Treatment. Proc Natl Acad Science, USA 95:5287-5292. 1998
Carlos M. Luque, Carmen M. Pérez-Ferreiro, Alicia Pérez-González, Ludwig Englmeier, Maria D. Koffa, and Isabel Correas. An Alternative Domain Containing a Leucine-rich Sequence Regulates Nuclear Cytoplasmic Localization of Protein 4.1R. J. Biol. Chem., Jan 2003; 278: 2686 - 2691. 2003

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GST-Tag antibody

Antibody sensitivity Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 4H3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 100ng, lane 2: 50ng, lane 3: 25ng, lane 4: 10ng, lane 5: 5ng, lane 6: 2ng, lane 7: 1ng Mouse IgG2a 4H3 Purified Sj E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

GST-Tag antibody

Western Blot Mouse IgG1 KAB29 Purified WB
0.1 mg / €360.00
  Acris Antibodies GmbH

GST-Tag antibody

Western blot using Affinity Purified anti-GST antibody to detect purified GST recombinant protein (Left) and in a lysate of HeLa cells expressing recombinant GST protein (Right). Approximately 0.1 μg of protein was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at RT followed by washes and reaction with a 1/5,000 dilution of IRDye(TM)800 conjugated Goat anti-Mouse IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Mouse IgG1 3D4 Purified C, E, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

GST-Tag antibody

Recombinant GST protein (20ng, Lane 1) and 293 cellular lysates transfected with a GST expression vector (20µg, Lane 2) were probed with anti-GST (1µg/ml). Arrow indicates GST (~26kDa) and GST-fustion protein (~29kDa). Mouse IgG2a GST.B6 Purified C, IP, WB
0.1 mg / €290.00
  Acris Antibodies GmbH

GST-Tag antibody

Figure 1. Western blot using GST Mab (Cat. #AM11012PU-N) to detect GST recombinant protein purified from bacterial lysate. Mouse IgG1 9AT106 Purified WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Goat Biotin Sj E, WB
1 mg / €510.00
  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Goat IgG Agarose
20 mg / €3,300.00
  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Goat IgG AP Sj E, IP, WB
1 mg / €620.00
  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Goat IgG FITC Sj F, ICC/IF
1 mg / €510.00
  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Goat IgG HRP C, Dot, E, IP, WB
1 mg / €510.00
  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Goat IgG TRITC Sj F
1 mg / €510.00
  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Goat IgG Texas Red Sj E, IP, WB
1 mg / €510.00
  Acris Antibodies GmbH

GST-Tag antibody

Western Blot analysis using anti-GST Pab (Cat. #AP11290PU-N) to detect a GST-fusion recombinant protein (42 kDa) purified from bacterial lysate (Lanes 1-3: 10, 40, and 160 ng GST-fusion protein). Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Goat Serum Sj E, IP, WB
2 ml / €440.00
  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Mouse IgG1 vpg66 Supernatant E, WB
2 ml / €450.00
  Acris Antibodies GmbH

GST-Tag antibody

Western blot analysis of GST ubiquitin recombinant protein probed with MOUSE ANTI GST:BIOTIN (SM1690B) followed by STREPTAVIDIN: HRP Mouse IgG1 vpg66 Biotin E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

GST-Tag antibody

Figure 1. Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Western blot was immunostained by anti-GST (S-tag-05). 
Lane 1: Affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose. 
Lane 2: Affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST antibody (S-tag-02). Mouse IgG2b S-tag-05 Purified E. coli, Mam, Sj IP, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

GST-Tag antibody

Immunocytochemistry/Immunofluorescence analysis of GST in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GST antibody (1/100) with Goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Mouse IgG2b 1E5 Purified Sj E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

GST-Tag antibody

Immunocytochemistry/Immunofluorescence analysis of GST in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GST antibody (1/100) with Goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Mouse IgG2b 1E5 Purified Sj E, ICC/IF, WB

Regular Price: 50 µl / €230.00

Special Price: 50 µl / €161.00

  Acris Antibodies GmbH

GST-Tag antibody

GST-Tag Mouse IgG1 S1 Hu WB
0.1 mg / €410.00
  Abnova Taiwan Corp.

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