Maltose Binding Protein Tag / MBP-Tag

Principal name

Maltose Binding Protein Tag / MBP-Tag antibody

Gene ID

948538 (malE)

Available reactivities

E. coli (Escherichia coli)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (capture) (E(capture))

Background of Maltose Binding Protein Tag / MBP-Tag antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

General readings

Fox JD., et al. (2001). Protein Sci. 10(3): 622-30
Riggs., et al. (2000). Mol. Biotechnol. 15: 51-63

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Maltose Binding Protein Tag / MBP-Tag antibody

Antibody sensitivity: MBP was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with MBP antibody (6E4) Cat.-No AM00088PU-N (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: 100ng MBP
Lane 2: 50ng
Lane 3: 25ng 
Lane 4: 10ng 
Lane 5: 5ng 
Lane 6: 2ng 
Lane 7: 1ng Mouse IgG1 6E4 Purified E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Western blot analysis: Recombinant protein MBP-tagged BCDEF (Lane 1), TPD52 (Lane 2) and MAGEA3 (Lane 3) (each 20 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 10C12 Purified E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Western blot analysis: Recombinant protein MBP-tagged BCDEF (Lane 1), TPD52 (Lane 2) and MAGEA3 (Lane 3) (each 20 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 10C12 Purified E, WB
50 µl / €230.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 ug of recombinant protein  containing the MBP epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit IgG Purified E, WB
1 mg / €510.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Mouse IgG1 R29 Purified IP, WB
0.25 mg / €450.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit Serum WB
0.1 ml / €450.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit C, WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit IgG E. coli E, E(capture), WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit IgG E. coli E, WB
1 mg / €730.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit IgG Serum WB
50 µl / €360.00
  Novus Biologicals Inc.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Mouse IgG1 R29 Aff - Purified IP, WB
0.125 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Maltose Binding Protein Tag / MBP-Tag

Maltose Binding Protein Tag / MBP-Tag E. coli Purified > 95 % by SDS PAGE E. coli
1 mg / €370.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag

Maltose Binding Protein Tag / MBP-Tag E. coli Purified > 95 % by SDS PAGE E. coli
0.5 mg / €230.00
  Acris Antibodies GmbH

13 Item(s)

per page
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