RFP-Tag

Principal name

RFP-Tag antibody

Ncbi ID

86600

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of RFP-Tag antibody

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified E, P, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg  of a HeLa lysate containing RFP recombinant protein. Separation was achieved using a 4-12% Bis Tris gradient gel followed by transfer to nitrocellulose and blocking.The membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Biotin C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of RFP recombinant protein detected withanti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDSPAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG FITC E, F, ICC/IF, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG HRP C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

RFP-Tag antibody

Western blot analysis with recombinant RFP.
Lane 1 : 10 ng.
Lane 2 : 50 ng.
Lane 3 : 250 ng.
Lane 4 : 1 µg. Rabbit IgG ICC/IF, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

RFP-Tag antibody

Western blot of RFP recombinant protein detected with RFP polyclonal antibody ( Cat # PAB11376 ).
Lane 1 shows no reaction against a GFP recombinant protein present in 10 µg of HeLa cell extract.
Lane 2 shows a single band detected in 10 µg of a HeLa lysate containing RFP recombinant protein as a 27 kDa band.
A 4-12% Bis-Tris gradient gel ( Invitrogen ) was used for SDS-PAGE. The membrane was blocked and then probed with RFP polyclonal antibody ( Cat # PAB11376 ) diluted 1:2,500 for 1 h at RT. Rabbit IgG Hu, Ms C, E, ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

6 Item(s)

per page
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