14-3-3 protein epsilon antibody

Principal name

14-3-3 protein epsilon antibody

Alternative names for 14-3-3 protein epsilon antibody

14-3-3E, YWHAE

SwissProt ID

P62258 (Human), P62259 (Mouse), P62260 (Rat), P62261 (Bovin), P62262 (Sheep), Q5ZMT0 (Chick)

Gene ID

7531 (YWHAE), 22627 (Ywhae), 443300

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of 14-3-3 protein epsilon antibody

The 14-3-3 family of proteins plays a key regulatory role in sigl transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, ß, ?, e, s, ?, t, and ? that have been identified in mammals. The initially described a and d isoforms are confirmed to be phosphorylated forms of ß and ?, respectively. Through their amino-termil a helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kises, phosphatases, and other sigling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed. 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions. 14-3-3 proteins may also induce target protein conformatiol changes that modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular sigls and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.

General readings

Oriente F, et al.(2005) J Biol Chem. ;280(49):40642-9.
Conklin D, et al.(2005) PNAS ,92(17):7892-7896.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein epsilon antibody

Immunohistochemistry analyzes of 14-3-3 ε antibody (Cat.-No.: AP06766PU-N) in Paraffin-Embedded Human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

Immunohistochemistry: AM09042PU-N YWHAE antibody staining of Formalin-Fixed, Paraffin-Embedded Human Lung at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a 5A5 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

Immunohistochemistry: AM09042PU-N YWHAE antibody staining of Formalin-Fixed, Paraffin-Embedded Human Lung at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a 5A5 Purified Hu, Ms E, P, WB

Regular Price: 50 µl / €230.00

Special Price: 50 µl / €161.00

  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon(GTX109090) antibody at 1:500 dilution. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

 
Sample size available for 25µl / 119€
Need more? Get any 5 sample sizes for only €420!
read more…

14-3-3 protein epsilon antibody

Immunohistochemical analysis of paraffin-embedded MDA-MB231 xenograft, using 14-3-3 epsilon(GTX113576) antibody at 1:500 dilution. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

 
Sample size available for 25µl / 119€
Need more? Get any 5 sample sizes for only €420!
read more…

14-3-3 protein epsilon antibody

Predicted band size: 29 kDa . Positive control: Human brain malignant glioma and laryngocarcinoma tissue . Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1:Human brain malignant glioma tissue lysate Lane2:Human laryngocarcinoma tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein epsilon antibody

Predicted band size: 29 kDa . Positive control: Human brain malignant glioma and laryngocarcinoma tissue . Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Human brain malignant glioma tissue lysate Lane2: Human laryngocarcinoma tissue lysate Lysates: 40 ug per lane Primary antibody: 1/2200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein epsilon (C-term) antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, P, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

Human Breast Epithelium stained with Rabbit anti 14-3-3 Epsilon antibody Cat.-No AP05568SU-N Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, P, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

14-3-3 protein epsilon (C-term) antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using GeneTex antibodies.
Lane 1 = anti-gamma 14-3-3 (GTX110629)
Lane 2 = anti-zeta 14-3-3 (GTX42902)
Lane 3 = anti-C-epsilon-14-3-3 (GTX42905)
Lane 4 = anti-N-epsilon-14-3-3 (GTX42906)
Lane 5 = anti-eta 14-3-3. (GTX42904)
The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 which migrates at 33kDa Rabbit Bov, Chk, Hu, Ms, Rb, Rt E, P, WB
0.1 ml / €510.00
  GeneTex Inc.

14-3-3 protein epsilon N-term antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using GeneTex antibodies.
Lane 1 = anti-gamma 14-3-3 (GTX110629)
Lane 2 = anti-zeta 14-3-3 (GTX42902)
Lane 3 = anti-C-epsilon-14-3-3 (GTX42905)
Lane 4 = anti-N-epsilon-14-3-3 (GTX42906)
Lane 5 = anti-eta 14-3-3. (GTX42904)
The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 which migrates at 33kDa Rabbit Bov, Chk, Hu, Ms, Rb, Rt E, P, WB
0.1 ml / €510.00
  GeneTex Inc.

14-3-3 protein epsilon antibody

Human NT-2, mouse brain extracts; . . Sample Type. . : 1. Human NT-2 cells (60ug). 2. mouse brain extracts (80ug). Primary Antibody Dilution: 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution: 1: 20,000. Image Submitted by: Yuzhi Chen. University of Arkansas for Medical Science .; YWHAE antibody - middle region (AVARP02058_P050) in Human NT-2, mouse brain extracts cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

14-3-3 protein epsilon pThr232 antibody

14-3-3 protein epsilon Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €250.00
  Bioss, Inc.

14-3-3 protein epsilon antibody

Western Blot: 14-3-3 episilon Antibody [NB100-92415] - Western blot (WB) analysis of 14-3-3 å (D238)  antibody in extracts from HepG2 cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

14-3-3 protein epsilon antibody

Immunohistochemistry: 14-3-3 epsilon Antibody [NBP1-32695] - analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

14-3-3 protein epsilon antibody

Western  Blot:  14-3-3 epsilon Antibody [NBP1-33554] – Western blot analysis of 14-3-3 epsilon on H1299 lysates using NBP1-33554 at a dilution of 1:1000. 30ug whole cell lysate loaded on 12% SDS PAGE gel. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

14-3-3 protein epsilon antibody

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-89827] - Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

17 Item(s)

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