14-3-3 protein epsilon antibody

Principal name

14-3-3 protein epsilon antibody

Alternative names for 14-3-3 protein epsilon antibody

14-3-3E, YWHAE

SwissProt ID

P62258 (Human), P62259 (Mouse), P62260 (Rat), P62261 (Bovin), P62262 (Sheep), Q5ZMT0 (Chick)

Gene ID

7531 (YWHAE), 22627 (Ywhae), 443300

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of 14-3-3 protein epsilon antibody

The 14-3-3 family of proteins plays a key regulatory role in sigl transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, ß, ?, e, s, ?, t, and ? that have been identified in mammals. The initially described a and d isoforms are confirmed to be phosphorylated forms of ß and ?, respectively. Through their amino-termil a helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kises, phosphatases, and other sigling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed. 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions. 14-3-3 proteins may also induce target protein conformatiol changes that modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular sigls and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.

General readings

Oriente F, et al.(2005) J Biol Chem. ;280(49):40642-9.
Conklin D, et al.(2005) PNAS ,92(17):7892-7896.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein epsilon antibody

Immunohistochemistry analyzes of 14-3-3 ε antibody (Cat.-No.: AP06766PU-N) in Paraffin-Embedded Human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

Immunohistochemistry: AM09042PU-N YWHAE antibody staining of Formalin-Fixed, Paraffin-Embedded Human Lung at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a 5A5 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

Immunohistochemistry: AM09042PU-N YWHAE antibody staining of Formalin-Fixed, Paraffin-Embedded Human Lung at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a 5A5 Purified Hu, Ms E, P, WB

Regular Price: 50 µl / €230.00

Special Price: 50 µl / €161.00

  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

Predicted band size: 29 kDa . Positive control: Human brain malignant glioma and laryngocarcinoma tissue . Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1:Human brain malignant glioma tissue lysate Lane2:Human laryngocarcinoma tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein epsilon antibody

Predicted band size: 29 kDa . Positive control: Human brain malignant glioma and laryngocarcinoma tissue . Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Human brain malignant glioma tissue lysate Lane2: Human laryngocarcinoma tissue lysate Lysates: 40 ug per lane Primary antibody: 1/2200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein epsilon (C-term) antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, P, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

Human Breast Epithelium stained with Rabbit anti 14-3-3 Epsilon antibody Cat.-No AP05568SU-N Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, P, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

14-3-3 protein epsilon (N-term) antibody

Human Breast (formalin-fixed, paraffin-embedded) stained with YWHAE antibody AP22883SU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Serum Sh E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

14-3-3 protein epsilon antibody

Western blot analysis of mouse brain extracts ( 50 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with YWHAE monoclonal antibody , clone 5A5 ( 1 : 1000 ) ( Cat # MAB1125 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a 5A5 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

14-3-3 protein epsilon antibody

Western blot analysis of extracts from HepG2/Jurkat cells, using 14-3-3 epsilon Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

14-3-3 protein epsilon antibody

Western blot analysis of extracts from HepG2/Jurkat cells, using 14-3-3 epsilon Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Mouse IgG2a 5A5 Purified Hu E, SDS-PAGE, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

13 Item(s)

per page
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