14-3-3 protein eta antibody

Principal name

14-3-3 protein eta antibody

Alternative names for 14-3-3 protein eta antibody

Protein AS1, YWHAH, YWHA1

SwissProt ID

P68509 (Bovin), P68510 (Mouse), P68511 (Rat), Q04917 (Human)

Gene ID

7533 (YWHAH)

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), Sh (Sheep), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of 14-3-3 protein eta antibody

The YWHAH gene product belongs to the 14-3-3 family of proteins which mediate sigl transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.This gene product belongs to the 14-3-3 family of proteins which mediate sigl transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

1. Sato S, Chiba T, Sakata E, Kato K, Mizuno Y, Hattori N, Tanaka K. 14-3-3eta is a novel regulator of parkin ubiquitin ligase. EMBO J. 2006 Jan 11;25(1):211-21. Epub 2005 Aug 11. PubMed PMID: 16096643.
2. Chen J, Lee CT, Errico SL, Becker KG, Freed WJ. Increases in expression of 14-3-3 eta and 14-3-3 zeta transcripts during neuroprotection induced by delta9-tetrahydrocannabinol in AF5 cells. J Neurosci Res. 2007 Jun;85(8):1724-33. PubMed PMID: 17455326.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein eta antibody

Western blot analysis of extracts from Jurkat cells, using 14-3-3 ? antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein eta antibody

WB Suggested Anti-YWHAH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein eta antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh P, WB
0.1 ml / €470.00
  Acris Antibodies GmbH

14-3-3 protein eta antibody

Western blot analysis of 14-3-3 eta antibody Cat.-No AP20467PU-N at 1/500 dilution: Lane 1: MCF-7 cell lysate. Lane 2: sp2/0 cell lysate. Lane 3: H9C2 cell lysate.  Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein eta (N-term) antibody

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with YWHAH antibody AP22684SU-N at 1/250 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh P, WB
50 µl / €470.00
  Acris Antibodies GmbH

14-3-3 protein eta antibody

YWHAH polyclonal antibody (A01), Lot # 060524JCS1 Western Blot analysis of YWHAH expression in SJCRH30 ( Cat # L027V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

14-3-3 protein eta antibody

Detection limit for recombinant GST tagged YWHAH is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 6A12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein eta antibody

14-3-3 protein eta Mouse IgG1 1C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein eta antibody

Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

14-3-3 protein eta antibody

Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

14-3-3 protein eta antibody

Immunohistochemical analysis of 14-3-3 eta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

14-3-3 protein eta antibody

Immunohistochemical analysis of 14-3-3 eta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

14-3-3 protein eta antibody

Immunohistochemical analysis of 14-3-3 eta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

14-3-3 protein eta antibody

WB: 14-3-3 ETA Antibody [NB100-65809] - WB of 14-3-3 isoforms in HEK293 cell extracts using AbD Serotec antibodies. Lane 1 = anti-gamma 14-3-3, anti-zeta 14-3-3(2), anti-C-epsilon-14-3-3(3), anti-N-epsilon-14-3-3(4), anti-eta 14-3-3(5). The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3. Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh P, WB
50 µl / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

14-3-3 protein eta

14-3-3 protein eta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

14-3-3 protein eta (1-246, His-tag)

14-3-3 eta, 1-246 aa, His-tagged: 15% SDS-PAGE (4 µg) Human Purified ≥ 95 by SDS-PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

14-3-3 protein eta (1-246, His-tag)

14-3-3 eta, 1-246 aa, His-tagged: 15% SDS-PAGE (4 µg) Human Purified ≥ 95 by SDS-PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

14-3-3 protein eta (1-246)

14-3-3 protein eta Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

14-3-3 protein eta (1-246)

14-3-3 protein eta Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

14-3-3 protein eta

14-3-3 protein eta Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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