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14-3-3 protein gamma antibody

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Principal name

14-3-3 protein gamma antibody

Alternative names for 14-3-3 protein gamma antibody

YWHAG, KCIP-1, Protein kinase C inhibitor protein 1

SwissProt ID

P61981 (Human), P61982 (Mouse), P61983 (Rat), P68252 (Bovin), P68253 (Sheep), Q5F3W6 (Chick)

Gene ID

7532 (YWHAG), 22628 (Ywhag), 56010

Ncbi ID

NP_036611, NP_036611.2, 9606, NM_018871, NM_019376, NP_061359.2

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of 14-3-3 protein gamma antibody

14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 gamma is thought to play an important role in muscle, being induced by growth factors in human vascular smooth muscle cells, and being expressed in skeletal and heart muscles. An example is the gamma isoform binds musclespecific receptor tyrosine kinase regulating synaptic gene transmission. The gamma isoform binds amyloid betaprotein precursor intracellular domain fragment (AICD) and FE65, facilitating FE65dependent gene transactivation. The binding of 14-3-3 gamma to glial fibrillary acidic protein is important in the regulation of glial filaments. 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. The gamma isoform is lowered in foetal Downs syndrome brains.

General readings

1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843.

AP20539PU-N - 14-3-3 protein gamma
Immunohistochemistry (IHC) analyzes of 14-3-3 gamma antibody (Cat.-No.: AP20539PU-N) in paraffin-embedded human brain tissue.
AP20539PU-N - 14-3-3 protein gamma
Western blot analysis of 14-3-3 gamma Antibody (Cat.-No AP20539PU-N) in extracts from Hela cells at 1/500 dilution.
C12004-1 - 14-3-3 protein gamma
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 14-3-3 gamma Antibody. The picture on the right is treated with the synthesized peptide.
C12004-1 - 14-3-3 protein gamma
Immunofluorescence analysis of COS7 cells, using 14-3-3 gamma Antibody. The picture on the right is treated with the synthesized peptide.
C12004-1 - 14-3-3 protein gamma
Western blot analysis of extracts from K562 cells, treated with insulin 0.01U/ml 15', using 14-3-3 gamma Antibody. The lane on the right is treated with the synthesized peptide.
H00007532-M01 - 14-3-3 protein gamma
Detection limit for recombinant GST tagged YWHAG is approximately 0.3ng/ml as a capture antibody.
H00007532-M02 - 14-3-3 protein gamma
YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in IMR-32 ( Cat # L008V1 ).
H00007532-M02 - 14-3-3 protein gamma
YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in PC-12 ( Cat # L012V1 ).
H00007532-M02 - 14-3-3 protein gamma
YWHAG monoclonal antibody (M02), clone 6A10 Western Blot analysis of YWHAG expression in K-562 ( Cat # L009V1 ).
H00007532-M02 - 14-3-3 protein gamma
YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in NIH/3T3 ( Cat # L018V1 ).
H00007532-M02 - 14-3-3 protein gamma
YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in Raw 264.7 ( Cat # L024V1 ).
H00007532-M02 - 14-3-3 protein gamma
Detection limit for recombinant GST tagged YWHAG is approximately 0.3ng/ml as a capture antibody.
MAB2048 - 14-3-3 protein gamma
The lysates of mouse brain ( lane 1, 30 µg ) and HeLa ( lane 2, 30 µg ) were resolved by SDS-PAGE and probed with YWHAG monoclonal antibody, clone J3H10 ( Cat # MAB2048 ) ( 1:1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
MAB2288 - 14-3-3 protein gamma
Western blot analysis of YWHAG in HeLa cell lysate ( Lane1 ), and bengamide treated lysates ( Lane 2 and 4, for 8h and 24h, respectively ) with YWHAG monoclonal antibody, clone HS23 ( Cat# MAB2288 ).
AP05567SU-N - 14-3-3 protein gamma
Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.Lane 1: anti-gamma 14-3-3 (AP05567SU-N)Lane 2: anti-zeta 14-3-3 (AP05572SU-N)Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)Lane 5: anti-eta 14-3-3. (AP05566SU-N)The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3
AP17846PU-N - 14-3-3 protein gamma
Western blot analysis of YWHAG Antibody (Center) (AP17846PU-N) in mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab.
AP17847PU-N - 14-3-3 protein gamma
Western blot analysis of YWHAG Antibody (N-term) (AP17847PU-N) in A375 cell line and mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab.
AR09101PU-L - 14-3-3 protein gamma
AR09101PU-L - 14-3-3 protein gamma
14-3-3 gamma, 1-247 aa: 15% SDS-PAGE (4 µg)
AM26569AF-N - 14-3-3 protein gamma
Immunoprecipitation of 14-3-3γ from A431 cells with mouse IgG2a (1) or AM26569AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26569AF-N.
AM26569AF-N - 14-3-3 protein gamma
Western blot analysis of 14-3-3γ expression in A431 (1), K562 (2), NIH/3T3 (3), mouse brain (4), PC12 (5) and Rat brain (6) using AM26569AF.
CPA2242-100ul - 14-3-3 protein gamma
Immunofluorescent analysis of 14-3-3 gamma staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2242-100ul - 14-3-3 protein gamma
Western blot analysis of 14-3-3 gamma expression in A549 (A); A431 (B); K562 (C); NIH3T3 (D); PC12 (E); rat liver (F) whole cell lysates.
TP301925 - 14-3-3 protein gamma
TA312741 - 14-3-3 protein gamma
Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 ? antibody.The lane on the right is treated with the synthesized peptide.
TA312741 - 14-3-3 protein gamma
Immunohistochemistry analysis of paraffin-embedded human brain tissue using 14-3-3 ? antibody.The picture on the right is treated with the synthesized peptide.
TA312741 - 14-3-3 protein gamma
Immunofluorescence analysis of COS7 cells, using 14-3-3 ? antibody.The picture on the right is treated with the synthesized peptide.
TA301660 - 14-3-3 protein gamma
14-3-3 gamma detected in lysates. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).
TA301660 - 14-3-3 protein gamma
Immunocytochemistry/Immunofluorescence: 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
TA301661 - 14-3-3 protein gamma
14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 & 3: bengamide treated lysates (8h and 24h, respectively).
TA301661 - 14-3-3 protein gamma
Immunocytochemistry/Immunofluorescence: Detection of 14-3-3 gamma (green) in HepG2 cells using TA301661. Nuclei (Blue) are counterstained with Hoechst 33258.
TA322404 - 14-3-3 protein gamma
Predicted band size: 28 kDa . Positive control: 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
TA322404 - 14-3-3 protein gamma
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using YWHAG antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA322405 - 14-3-3 protein gamma
Predicted band size: 28 kDa . Positive control: Hela, 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/1800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
TA322405 - 14-3-3 protein gamma
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using YWHAG antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification: x200)

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein gamma antibody
  Mouse Monoclonal Antibody against 14-3-3 gamma (HS23)  
14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 & 3: bengamide treated lysates (8h and 24h, respectively). Mouse IgG1 HS23 Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, WB
0.1 ml / €451.00
  OriGene Technologies, Inc.
+1 additional image

14-3-3 protein gamma antibody
  Rabbit polyclonal anti-14-3-3 gamma antibody  
Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 ? antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

14-3-3 protein gamma antibody
  Anti-YWHAG Rabbit Polyclonal Antibody  
Predicted band size: 28 kDa . Positive control: 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

14-3-3 protein gamma antibody
  Anti-YWHAG Rabbit Polyclonal Antibody  
Predicted band size: 28 kDa . Positive control: Hela, 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/1800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

14-3-3 protein gamma (1-12) antibody
Immunoprecipitation of 14-3-3γ from A431 cells with mouse IgG2a (1) or AM26569AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26569AF-N. Mouse IgG2a KC21 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

14-3-3 protein gamma antibody
Western blot analysis of YWHAG Antibody (Center) (AP17846PU-N) in mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

14-3-3 protein gamma (N-term) antibody
Western blot analysis of YWHAG Antibody (N-term) (AP17847PU-N) in A375 cell line and mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody
Immunohistochemistry (IHC) analyzes of 14-3-3 gamma antibody (Cat.-No.: AP20539PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

8 Item(s)

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