14-3-3 protein gamma antibody

Principal name

14-3-3 protein gamma antibody

Alternative names for 14-3-3 protein gamma antibody

YWHAG, KCIP-1, Protein kinase C inhibitor protein 1

SwissProt ID

P61981 (Human), P61982 (Mouse), P61983 (Rat), P68252 (Bovin), P68253 (Sheep), Q5F3W6 (Chick)

Gene ID

7532 (YWHAG)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), Sh (Sheep), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of 14-3-3 protein gamma antibody

14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 gamma is thought to play an important role in muscle, being induced by growth factors in human vascular smooth muscle cells, and being expressed in skeletal and heart muscles. An example is the gamma isoform binds musclespecific receptor tyrosine kinase regulating synaptic gene transmission. The gamma isoform binds amyloid betaprotein precursor intracellular domain fragment (AICD) and FE65, facilitating FE65dependent gene transactivation. The binding of 14-3-3 gamma to glial fibrillary acidic protein is important in the regulation of glial filaments. 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. The gamma isoform is lowered in foetal Downs syndrome brains.

General readings

1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein gamma antibody

YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in IMR-32 ( Cat # L008V1 ). Mouse IgG2b 6A10 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein gamma antibody

14-3-3 gamma detected in lysates. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively). Mouse IgG2a KC21 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

14-3-3 protein gamma antibody

14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 & 3: bengamide treated lysates (8h and 24h, respectively). Mouse IgG1 HS23 Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein gamma antibody

Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 ? antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein gamma antibody

Predicted band size: 28 kDa . Positive control: 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein gamma antibody

Predicted band size: 28 kDa . Positive control: Hela, 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/1800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein gamma (1-12) antibody

Immunoprecipitation of 14-3-3? from A431 cells with mouse IgG2a (1) or AM26569AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26569AF-N. Mouse IgG2a KC21 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG1 AT4B9 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG1 AT4B9 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit IgG Serum Bov, Chk, Hu, Ms, Rt, Sh E, P, WB
0.1 ml / €470.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

Western blot analysis of YWHAG Antibody (Center) (AP17846PU-N) in mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

14-3-3 protein gamma (N-term) antibody

Western blot analysis of YWHAG Antibody (N-term) (AP17847PU-N) in A375 cell line and mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

Immunohistochemistry (IHC) analyzes of 14-3-3 gamma antibody (Cat.-No.: AP20539PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

14-3-3 protein gamma antibody

14-3-3 protein gamma Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

14-3-3 protein gamma antibody

Detection limit for recombinant GST tagged YWHAG is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 3G3 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG2a 1B4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein gamma antibody

The lysates of  mouse brain ( lane 1, 30 µg ) and HeLa ( lane 2, 30 µg ) were resolved by SDS-PAGE and probed with YWHAG monoclonal antibody, clone J3H10 ( Cat # MAB2048 ) ( 1:1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b J3H10 Hu, Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

14-3-3 protein gamma antibody

Western blot analysis of YWHAG in HeLa cell lysate ( Lane1 ), and bengamide treated lysates ( Lane 2 and 4, for 8h and 24h, respectively ) with YWHAG monoclonal antibody, clone HS23 ( Cat# MAB2288 ). Mouse IgG1 HS23 Bov, Chk, Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

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