14-3-3 protein gamma antibody

Principal name

14-3-3 protein gamma antibody

Alternative names for 14-3-3 protein gamma antibody

YWHAG, KCIP-1, Protein kinase C inhibitor protein 1

SwissProt ID

P61981 (Human), P61982 (Mouse), P61983 (Rat), P68252 (Bovin), P68253 (Sheep), Q5F3W6 (Chick)

Gene ID

7532 (YWHAG), 22628 (Ywhag), 56010

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of 14-3-3 protein gamma antibody

14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 gamma is thought to play an important role in muscle, being induced by growth factors in human vascular smooth muscle cells, and being expressed in skeletal and heart muscles. An example is the gamma isoform binds musclespecific receptor tyrosine kinase regulating synaptic gene transmission. The gamma isoform binds amyloid betaprotein precursor intracellular domain fragment (AICD) and FE65, facilitating FE65dependent gene transactivation. The binding of 14-3-3 gamma to glial fibrillary acidic protein is important in the regulation of glial filaments. 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. The gamma isoform is lowered in foetal Downs syndrome brains.

General readings

1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein gamma antibody

  Mouse Monoclonal Antibody against 14-3-3 gamma (KC21)  
14-3-3 gamma detected in lysates. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively). Mouse IgG2a KC21 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

14-3-3 protein gamma antibody

  Mouse Monoclonal Antibody against 14-3-3 gamma (HS23)  
14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 & 3: bengamide treated lysates (8h and 24h, respectively). Mouse IgG1 HS23 Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein gamma antibody

  Rabbit polyclonal anti-14-3-3 gamma antibody  
Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 ? antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein gamma antibody

  Anti-YWHAG Rabbit Polyclonal Antibody  
Predicted band size: 28 kDa . Positive control: 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein gamma antibody

  Anti-YWHAG Rabbit Polyclonal Antibody  
Predicted band size: 28 kDa . Positive control: Hela, 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/1800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein gamma (1-12) antibody

Immunoprecipitation of 14-3-3γ from A431 cells with mouse IgG2a (1) or AM26569AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26569AF-N. Mouse IgG2a KC21 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG1 AT4B9 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG1 AT4B9 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit IgG Serum Bov, Chk, Hu, Ms, Rt, Sh E, P, WB
0.1 ml / €470.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

Western blot analysis of YWHAG Antibody (Center) (AP17846PU-N) in mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

14-3-3 protein gamma (N-term) antibody

Western blot analysis of YWHAG Antibody (N-term) (AP17847PU-N) in A375 cell line and mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

14-3-3 protein gamma antibody

Immunohistochemistry (IHC) analyzes of 14-3-3 gamma antibody (Cat.-No.: AP20539PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Ywhag - mouse gene knockout kit via CRISPR

Ywhag - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ywhag - mouse gene knockout kit via CRISPR

Ywhag - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ywhag - mouse gene knockout kit via CRISPR

Ywhag - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ywhag - mouse gene knockout kit via CRISPR

Ywhag - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

14-3-3 protein gamma

  Recombinant protein of human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG)  
14-3-3 protein gamma Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

14-3-3 protein gamma (1-247)

14-3-3 protein gamma Human Purified ≥ 95 by SDS-PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

14-3-3 protein gamma (1-247)

14-3-3 protein gamma Human Purified ≥ 95 by SDS-PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ywhag (GFP-tagged) - Mouse tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (Ywhag), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ywhag (GFP-tagged) - Mouse tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (Ywhag), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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