14-3-3 protein sigma / SFN antibody

Principal name

14-3-3 protein sigma / SFN antibody

Alternative names for 14-3-3 protein sigma / SFN antibody

Stratifin, HME1, Epithelial cell marker protein 1

SwissProt ID

O70456 (Mouse), O77642 (Sheep), P31947 (Human), Q0VC36 (Bovin)

Gene ID

2810 (SFN), 443032

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Por (Porcine), Ze (Zebrafish), Ce (C. elegans), Eq (Equine), GP (Guinea Pig), Amph (Amphibian), Op (Opossum)

Available hosts

Mouse, Goat, Rabbit, Sheep

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), PLA/Proximity ligation assay (PLA), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of 14-3-3 protein sigma / SFN antibody

It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1.

General readings

1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E.,
Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive
epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C
signalling pathway.J. Molec. Biol. 231: 982-998, 1993.
2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets
14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002.
3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng,
Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to
facilitate cytokinesis.Nature 446: 329-332, 2007.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein sigma / SFN antibody

Western blot analysis of extracts of various cell lines, using SFN antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SFN Rabbit Polyclonal antibody. (TA590217) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3C3 Hu P, WB
50 µg / €530.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN (C-term) antibody

Flow cytometric analysis of Hela cells using SFN Antibody (C-term) Cat.-No AP33512PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Rabbit Ig Purified Hu F, ICC/IF, P, WB
0.4 ml / €390.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN antibody

TA302401 (0.03 ug/ml) staining of Human Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

Western blot analysis of extracts from HT-29 cells and RAW264.7 cells, using 14-3-3 s antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

SFN Antibody (Center) (Cat. #TA324655) western blot analysis in A431 cell line and mouse bladder tissue lysates (35ug/lane).This demonstrates the SFN antibody detected the SFN protein (arrow). Rabbit IgG Purified Bov, Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

WB Suggested Anti-SFN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Placenta Rabbit IgG Purified Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

WB Suggested Anti-SFN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T Rabbit IgG Purified Can, Eq, GP, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

Western Blot: 14-3-3 sigma/Stratifin Antibody [TA337133] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody.  Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue p Rabbit Purified Amph, Bov, Can, Eq, Hu, Op P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh WB
0.1 ml / €450.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN (N-term) antibody

Human Skin (formalin-fixed, paraffin-embedded) stained with SFN antibody AP22876SU-N at 1/250 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Serum Hu, Sh P, WB
50 µl / €470.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN pSer248 antibody

14-3-3 protein sigma / SFN Rabbit Serum Hu E, ICC/IF, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Rabbit Serum Hu, Ms, Rt C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN antibody

SFN MaxPab rabbit polyclonal antibody. Western Blot analysis of SFN expression in human colon. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN antibody

Immunoperoxidase of monoclonal antibody to SFN on formalin-fixed paraffin-embedded human uterine cervix tissue. [antibody concentration 5ug/ml] Mouse IgG1 3C3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN antibody

SFN monoclonal antibody (M02), clone 2G2. Western Blot analysis of SFN expression in U-2 OS ( Cat # L022V1 ). Mouse IgG2a 2G2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN antibody

SFN monoclonal antibody (M05), clone 1E6. Western Blot analysis of SFN expression in human ovary cancer. Mouse IgG2a 1E6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN antibody

SFN monoclonal antibody (M06), clone 1E7. Western Blot analysis of SFN expression in HepG2 ( Cat # L019V1 ). Mouse IgG2a 1E7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN antibody

SFN polyclonal antibody ( Cat # PAB6932 ) ( 2.5µg/ml ) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

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