14-3-3 protein sigma / SFN antibody

Principal name

14-3-3 protein sigma / SFN antibody

Alternative names for 14-3-3 protein sigma / SFN antibody

Stratifin, HME1, Epithelial cell marker protein 1

Selected pictures

14-3-3 protein sigma / SFN antibody - H00002810-M01
H00002810-M01 - SFN monoclonal antibody (M01), clone 3C3 Western Blot analysis of SFN expression in HeLa ( Cat # L013V1 ).
14-3-3 protein sigma / SFN antibody - H00002810-M01
H00002810-M01 - Immunoperoxidase of monoclonal antibody to SFN on formalin-fixed paraffin-embedded human uterine cervix tissue. [antibody concentration 5ug/ml]

SwissProt ID

O70456 (Mouse), O77642 (Sheep), P31947 (Human), Q0VC36 (Bovin)

Gene ID

2810 (SFN), 443032

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Por (Porcine), Ze (Zebrafish), Ce (C. elegans)

Available hosts

Mouse, Rabbit, Goat, Chicken, Sheep

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), PLA/Proximity ligation assay (PLA), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of 14-3-3 protein sigma / SFN antibody

It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1.

General readings

1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E.,
Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive
epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C
signalling pathway.J. Molec. Biol. 231: 982-998, 1993.
2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets
14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002.
3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng,
Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to
facilitate cytokinesis.Nature 446: 329-332, 2007.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
AM20889PU-N

14-3-3 protein sigma / SFN antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3C3 Hu P, WB
50 µg / €530.00
  Acris Antibodies GmbH
GTX100289

14-3-3 protein sigma / SFN antibody

14-3-3 sigma antibody detects SFN protein by Western blot analysis.A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract12 % SDS-PAGE14-3-3 sigma antibody (GTX100289) dilution: 1:3000 Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

Sample size available for 25µl / 119€
Buy any 2 samples and get a 15 % discount (read more…)

GTX100801

14-3-3 protein sigma / SFN antibody

14-3-3 sigma antibody detects SFN protein by Western blot analysis.A. 30 µg 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extractE. 30 ug A375 whole cell lysate/extract12 % SDS-PAGE14-3-3 sigma antibody (GTX100801) dilution: 1:2000 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €350.00
  GeneTex Inc.

Sample size available for 25µl / 119€
Buy any 2 samples and get a 15 % discount (read more…)

AM00494PU-N

14-3-3 protein sigma / SFN antibody

Formalin-fixed, paraffin-embedded human skin stained with AM00494PU-N using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis. Mouse IgG1 1433S01 Purified Hu ICC/IF, IP, P, WB
0.1 mg / €480.00
  Acris Antibodies GmbH
AM32543PU-N

14-3-3 protein sigma / SFN (incl. pos. control) antibody

Immunoblot Analysis (Representative Blot from a previous lot): 
EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-14-3-3σ (0.5 μg/ml). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and a chemilumin-escence
detection system. Arrow indicates 14-3-3σ (~30kDa). Mouse IgG1 CS112-2A8 Purified Hu, Ms, Rt IP, WB
0.2 mg / €440.00
  Acris Antibodies GmbH
AP05570SU-N

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh WB
0.1 ml / €450.00
  Acris Antibodies GmbH
AP16443PU-N

14-3-3 protein sigma / SFN antibody

AP16443PU-N (2.5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH
AP22876SU-N

14-3-3 protein sigma / SFN (N-term) antibody

Human Skin (formalin-fixed, paraffin-embedded) stained with SFN antibody AP22876SU-N at 1/250 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Serum Hu, Sh P, WB
50 µl / €470.00
  Acris Antibodies GmbH
AP32566SU-N

14-3-3 protein sigma / SFN (N-term) antibody

14-3-3 protein sigma / SFN Rabbit Serum Bov, Chk, Hu, Ms, Rt, Sh WB
0.1 ml / €430.00
  Acris Antibodies GmbH
AP54745SU-N

14-3-3 protein sigma / SFN pSer248 antibody

14-3-3 protein sigma / SFN Rabbit Serum Hu E, ICC/IF, WB
0.2 ml / €400.00
  Acris Antibodies GmbH
AP54746SU-N

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Rabbit Serum Hu, Ms, Rt C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH
GTX14116

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Mouse IgG 1.N.6 Purified Hu IP, P, WB
50 µg / €380.00
  GeneTex Inc.
GTX14122

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Mouse IgG1 1.T.28 Purified Hu, Ms, Rt WB

Regular Price: 50 µg / €320.00

Special Price: 50 µg / €210.00

  GeneTex Inc.
GTX14123

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Mouse IgG1 1.N.6 Purified Hu ICC/IF, IP, P, WB
0.25 ml / €350.00
  GeneTex Inc.
GTX42903

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Rabbit Bov, Chk, Hu, Ms, Rb, Rt WB
0.1 ml / €490.00
  GeneTex Inc.
GTX42912

14-3-3 protein sigma / SFN antibody

Western blot with Sheep anti-14-3-3 (pSER185) antibody (GTX42912)Lane 1, 2ug recombinant 14-3-3 betaLane 2, 2ug recombinant 14-3-3 zeta Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated Sheep Bov, Hu, Ms, Rb, Rt WB
0.1 mg / €490.00
  GeneTex Inc.
GTX63531

14-3-3 protein sigma / SFN antibody

Western blot analysis on (A) fetal lung, (B) A549, (C) human kidney, and (D) human spleen lysates using anti-14-3-3 sigma RabMAb (cat. #GTX63531). Rabbit IgG EPR5872 Hu, Ms, Rt WB
0.1 ml / €420.00
  GeneTex Inc.
GTX82379

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Chicken Aff - Purified Hu, Ms, Rt WB
50 µg / €500.00
  GeneTex Inc.
GTX89431

14-3-3 protein sigma / SFN Internal antibody

GTX89431 (0.1µg/ml) staining of human skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Can, Hu, Ms, Por, Rt C, E, WB
0.1 mg / €360.00
  GeneTex Inc.
GTX112949

14-3-3 protein sigma / SFN antibody

Sample (30 ug of whole cell lysate) A: H1299  B: Hela  12% SDS PAGE GTX112949 diluted at 1:1000 Rabbit Aff - Purified Hu P, WB
0.1 ml / €350.00
  GeneTex Inc.

Sample size available for 25µl / 119€
Buy any 2 samples and get a 15 % discount (read more…)

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