14-3-3 protein sigma / SFN antibody

Principal name

14-3-3 protein sigma / SFN antibody

Alternative names for 14-3-3 protein sigma / SFN antibody

Stratifin, HME1, Epithelial cell marker protein 1

SwissProt ID

O70456 (Mouse), O77642 (Sheep), P31947 (Human), Q0VC36 (Bovin)

Gene ID

2810 (SFN), 443032

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Por (Porcine), Ze (Zebrafish), Ce (C. elegans)

Available hosts

Mouse, Goat, Rabbit, Chicken, Sheep

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), PLA/Proximity ligation assay (PLA), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of 14-3-3 protein sigma / SFN antibody

It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1.

General readings

1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E.,
Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive
epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C
signalling pathway.J. Molec. Biol. 231: 982-998, 1993.
2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets
14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002.
3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng,
Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to
facilitate cytokinesis.Nature 446: 329-332, 2007.

Items 1 to 20 of 48 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein sigma / SFN antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SFN Rabbit Polyclonal antibody. (TA590217) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3C3 Hu P, WB
50 µg / €530.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN antibody

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using 14-3-3 sigma(GTX100289) antibody at 1:100 dilution. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB

Regular Price: 0.1 ml / €350.00

Special Price: 0.1 ml / €297.50

  GeneTex Inc.


Sample size available for 25µl / 119€
read more…

14-3-3 protein sigma / SFN antibody

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma(GTX100801) antibody at 1:100 dilution. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB

Regular Price: 0.1 ml / €350.00

Special Price: 0.1 ml / €297.50

  GeneTex Inc.


Sample size available for 25µl / 119€
read more…

14-3-3 protein sigma / SFN antibody

Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig( SFN Antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9). Mouse IgG1 3F10D9 Purified Hu E, P, WB
0.15 ml / €285.00
  Proteintech Group, Inc.

14-3-3 protein sigma / SFN antibody

AP16443PU-N (2.5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN antibody

Formalin-fixed, paraffin-embedded human skin stained with AM00494PU-N using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis. Mouse IgG1 1433S01 Purified Hu ICC/IF, IP, P, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN (incl. pos. control) antibody

Immunoblot Analysis (Representative Blot from a previous lot): 
EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-14-3-3σ (0.5 μg/ml). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and a chemilumin-escence
detection system. Arrow indicates 14-3-3σ (~30kDa). Mouse IgG1 CS112-2A8 Purified Hu, Ms, Rt IP, WB
0.2 mg / €440.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh WB
0.1 ml / €450.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN (N-term) antibody

Human Skin (formalin-fixed, paraffin-embedded) stained with SFN antibody AP22876SU-N at 1/250 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Serum Hu, Sh P, WB
50 µl / €470.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN (N-term) antibody

14-3-3 protein sigma / SFN Rabbit Serum Bov, Chk, Hu, Ms, Rt, Sh WB
0.1 ml / €440.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN pSer248 antibody

14-3-3 protein sigma / SFN Rabbit Serum Hu E, ICC/IF, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Rabbit Serum Hu, Ms, Rt C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Mouse IgG 1.N.6 Purified Hu IP, P, WB

Regular Price: 50 µg / €380.00

Special Price: 50 µg / €323.00

  GeneTex Inc.

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Mouse IgG1 1.T.28 Purified Hu, Ms, Rt WB

Regular Price: 50 µg / €320.00

Special Price: 50 µg / €240.00

  GeneTex Inc.

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Mouse IgG1 1.N.6 Purified Hu ICC/IF, IP, P, WB

Regular Price: 0.25 ml / €350.00

Special Price: 0.25 ml / €297.50

  GeneTex Inc.

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Rabbit Bov, Chk, Hu, Ms, Rb, Rt WB

Regular Price: 0.1 ml / €490.00

Special Price: 0.1 ml / €416.50

  GeneTex Inc.

14-3-3 protein sigma / SFN antibody

Western blot with Sheep anti-14-3-3 (pSER185) antibody (GTX42912)Lane 1, 2ug recombinant 14-3-3 betaLane 2, 2ug recombinant 14-3-3 zeta Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated Sheep Bov, Hu, Ms, Rb, Rt WB

Regular Price: 0.1 mg / €490.00

Special Price: 0.1 mg / €416.50

  GeneTex Inc.

14-3-3 protein sigma / SFN antibody

Western blot analysis on (A) fetal lung, (B) A549, (C) human kidney, and (D) human spleen lysates using anti-14-3-3 sigma RabMAb (cat. #GTX63531). Rabbit IgG EPR5872 Hu, Ms, Rt WB
0.1 ml / €420.00
  GeneTex Inc.

14-3-3 protein sigma / SFN antibody

14-3-3 protein sigma / SFN Chicken Aff - Purified Hu, Ms, Rt WB
50 µg / €500.00
  GeneTex Inc.

Items 1 to 20 of 48 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • Pinterest
  • LinkedIn