14-3-3 protein theta antibody

Principal name

14-3-3 protein theta antibody

Alternative names for 14-3-3 protein theta antibody

14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1

SwissProt ID

P27348 (Human)

Gene ID

10971 (YWHAQ)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Ze (Zebrafish), African clawed frog, Por (Porcine), Mky (Monkey)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Flow Cytometry (F), Frozen Sections (C)

Background of 14-3-3 protein theta antibody

At least seven isoforms comprise the highly conserved 14-3-3 family of homo-and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. These multifunctional proteins bind and modulate the function of a wide array of cellular proteins. They exist mainly as dimers with a monomeric molecular mass of approximately 30 kDa and have an acidic isoelectric point of 4.5-5. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with bad, prevents apoptosis.3 14-3-3 plays a dual role in Raf-1 activation. It helps to keep Raf-1 in an inactive state by binding to phophoserine-259 and the cysteine-rich domain (DRD). Interactions of 14-3-3 at the phosphoserine-621 may be required for Raf-1 activation and may function as an essential cofactor for Raf-1 kinase activity.3 Other proteins that have been shown to bind 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, middle-T antigen, KSR, IGF-1 receptor, etc.3 Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases. The theta or tau isoform was initially isolated from T cells.1 It

General readings

Howlett KF, Sakamoto K, Garnham A, Cameron-Smith D, Hargreaves M.
Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle.
Diabetes. 2007 Jun;56(6):1608-14. Epub 2007 Mar 16.
PMID: 17369524

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein theta antibody

Figure 1. A typical Western blot (10% SDS-PAGE) of 3T3 (Lane 1), PC12 (Lane 2), MDBK (Lane 3), Jurkat (Lane 4) and normal human fibroblasts (Lane 5). Mouse IgG1 3B9 Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

14-3-3 protein theta (1-245) antibody

Immunohistochemistry: AM09060PU-N 14-3-3 tau antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex. Mouse IgG1 AT1A1 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

14-3-3 protein theta (1-245) antibody

Immunohistochemistry: AM09060PU-N 14-3-3 tau antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex. Mouse IgG1 AT1A1 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

14-3-3 protein theta antibody

TA303297 (0.03µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

TA303300 (0.01µg/ml) staining of mouse eye lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

Western blot analysis of extracts from HUVEC cells and HeLa cells, using 14-3-3 ? antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

Predicted band size: 28 kDa . Positive control: Mouse brain tissue, A549 cell and human lymphoma tissue, hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: %SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: A549 cell lysate Lane3:Human lymphoma tissue lysate Lane4:Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/950 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

Predicted band size: 28 kDa . Positive control: Mouse brain tissue, A549 cell and human lymphoma tissue, NIH/3T3 cell and human colon cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: %SDS-PAGE Lane1:Mouse brain tissue lysate Lane2: A549 cell lysate Lane3:Human lymphoma tissue lysate Lane4: NIH/3T3 cell lysate Lane5:Human colon cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

Western blot analysis of extracts of various cell lines, using YWHAQ antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

WB Suggested Anti-YWHAQ Antibody Titration: 0.2-1 ug/ml; Positive Control: SW620 cell lysateYWHAQ is strongly supported by BioGPS gene expression data to be expressed in Human SW620 cells Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

14-3-3 protein theta antibody

Immunohistochemistry analsyis of 14-3-3 protein theta Antibody (Cat.-No AP06768PU-N) in Paraffin Embedded Human brain tissue Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein theta antibody

Western blot analysis of 14-3-3 theta antibody (Cat.-No AP20329PU-N) at 1/500 dilution: Lane 1: Hela cell lysate. Lane 2: sp2/0 cell lysate. Lane 3: H9C2 cell lysate. Rabbit Aff - Purified Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein theta antibody

14-3-3 protein theta Mouse IgG1 3B9 Bov, Hu, Ms, Rt IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

14-3-3 protein theta antibody

Cell lysate of Jurkat ( 20 µg ) was resolved by SDS-PAGE, transferred to NC membrane and probed with YWHAQ monoclonal antibody, clone AT1A1 ( Cat # MAB2098 ) ( 1:1000 ). Mouse IgG1 AT1A1 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

14-3-3 protein theta antibody

YWHAQ polyclonal antibody ( Cat # PAB7392 ) ( 0.03 µg/ml ) staining of human brain ( hippocampus ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

14-3-3 protein theta antibody

Western blot analysis of YWHAQ expression in HeLa ( Lane 1 ) and Jurkat ( Lane 2 ) whole cell lysates. Using YWHAQ polyclonal antibody ( Cat # PAB14303 ). Rabbit Hu, Ms, Rt C, IP, WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

14-3-3 protein theta pSer232 antibody

Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

14-3-3 protein theta pSer232 antibody

Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

14-3-3 protein theta antibody

Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Ab-232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

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