14-3-3 protein theta antibody

Principal name

14-3-3 protein theta antibody

Alternative names for 14-3-3 protein theta antibody

14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1

SwissProt ID

P27348 (Human)

Gene ID

10971 (YWHAQ)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Ze (Zebrafish), African clawed frog, Por (Porcine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)

Background of 14-3-3 protein theta antibody

14-3-3 theta is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple cell signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 tau/theta is primarily expressed in Tcells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.

General readings

Howlett KF, Sakamoto K, Garnham A, Cameron-Smith D, Hargreaves M.
Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle.
Diabetes. 2007 Jun;56(6):1608-14. Epub 2007 Mar 16.
PMID: 17369524

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein theta antibody

Figure 1. A typical Western blot (10% SDS-PAGE) of 3T3 (Lane 1), PC12 (Lane 2), MDBK (Lane 3), Jurkat (Lane 4) and normal human fibroblasts (Lane 5). Mouse IgG1 3B9 Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

14-3-3 protein theta (1-245) antibody

Immunohistochemistry: AM09060PU-N 14-3-3 tau antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex. Mouse IgG1 AT1A1 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

14-3-3 protein theta (1-245) antibody

Immunohistochemistry: AM09060PU-N 14-3-3 tau antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex. Mouse IgG1 AT1A1 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

14-3-3 protein theta antibody

TA303297 (0.03µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

TA303300 (0.01µg/ml) staining of mouse eye lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

Western blot analysis of extracts from HUVEC cells and HeLa cells, using 14-3-3 ? antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

Predicted band size: 28 kDa . Positive control: Mouse brain tissue, A549 cell and human lymphoma tissue, hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: %SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: A549 cell lysate Lane3:Human lymphoma tissue lysate Lane4:Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/950 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

Predicted band size: 28 kDa . Positive control: Mouse brain tissue, A549 cell and human lymphoma tissue, NIH/3T3 cell and human colon cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: %SDS-PAGE Lane1:Mouse brain tissue lysate Lane2: A549 cell lysate Lane3:Human lymphoma tissue lysate Lane4: NIH/3T3 cell lysate Lane5:Human colon cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

Western blot analysis of extracts of various cell lines, using YWHAQ antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

WB Suggested Anti-YWHAQ Antibody Titration: 0.2-1 ug/ml; Positive Control: SW620 cell lysateYWHAQ is strongly supported by BioGPS gene expression data to be expressed in Human SW620 cells Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit IgG Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

14-3-3 protein theta antibody

Immunohistochemistry analsyis of 14-3-3 protein theta Antibody (Cat.-No AP06768PU-N) in Paraffin Embedded Human brain tissue Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein theta antibody

Western blot analysis of 14-3-3 theta antibody (Cat.-No AP20329PU-N) at 1/500 dilution: Lane 1: Hela cell lysate. Lane 2: sp2/0 cell lysate. Lane 3: H9C2 cell lysate. Rabbit Aff - Purified Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein theta antibody

14-3-3 protein theta Mouse IgG1 3B9 Bov, Hu, Ms, Rt IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

14-3-3 protein theta antibody

Cell lysate of Jurkat ( 20 µg ) was resolved by SDS-PAGE, transferred to NC membrane and probed with YWHAQ monoclonal antibody, clone AT1A1 ( Cat # MAB2098 ) ( 1:1000 ). Mouse IgG1 AT1A1 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

14-3-3 protein theta antibody

YWHAQ polyclonal antibody ( Cat # PAB7392 ) ( 0.03 µg/ml ) staining of human brain ( hippocampus ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

14-3-3 protein theta antibody

Western blot analysis of YWHAQ expression in HeLa ( Lane 1 ) and Jurkat ( Lane 2 ) whole cell lysates. Using YWHAQ polyclonal antibody ( Cat # PAB14303 ). Rabbit Hu, Ms, Rt C, IP, WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

14-3-3 protein theta pSer232 antibody

Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

14-3-3 protein theta pSer232 antibody

Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

14-3-3 protein theta antibody

Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Ab-232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

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