Alternative names for 14-3-3 protein theta antibody
14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1
Anti-14-3-3 theta/tau antibody [3B9] used in Western blot. GTX10439 - At right is a typical western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3), Jurkat (lane 4) and normal human fibroblasts (lane 5).
AP06768PU-N 14-3-3 protein theta - Immunohistochemistry analsyis of 14-3-3 protein theta Antibody (Cat.-No AP06768PU-N) in Paraffin Embedded Human brain tissue
Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Ze (Zebrafish), African clawed frog, Por (Porcine), Mky (Monkey)
Mouse, Rabbit, Goat
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C)
Background of 14-3-3 protein theta antibody
At least seven isoforms comprise the highly conserved 14-3-3 family of homo-and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. These multifunctional proteins bind and modulate the function of a wide array of cellular proteins. They exist mainly as dimers with a monomeric molecular mass of approximately 30 kDa and have an acidic isoelectric point of 4.5-5. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with bad, prevents apoptosis.3 14-3-3 plays a dual role in Raf-1 activation. It helps to keep Raf-1 in an inactive state by binding to phophoserine-259 and the cysteine-rich domain (DRD). Interactions of 14-3-3 at the phosphoserine-621 may be required for Raf-1 activation and may function as an essential cofactor for Raf-1 kinase activity.3 Other proteins that have been shown to bind 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, middle-T antigen, KSR, IGF-1 receptor, etc.3 Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases. The theta or tau isoform was initially isolated from T cells.1 It
Howlett KF, Sakamoto K, Garnham A, Cameron-Smith D, Hargreaves M. Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle. Diabetes. 2007 Jun;56(6):1608-14. Epub 2007 Mar 16. PMID: 17369524
At right is a typical western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3), Jurkat (lane 4) and normal human fibroblasts (lane 5).
Human SW620; WB Suggested Anti-YWHAQ Antibody Titration: 0.2-1 ug/ml. Positive Control: SW620 cell lysate. YWHAQ is strongly supported by BioGPS gene expression data to be expressed in Human SW620 cells.; YWHAQ antibody - N-terminal region (AVARP02062_P050) in Human SW620 cells using Western Blot
Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Tau protein Polyclonal Antibody (bs-0157R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Tau protein Polyclonal Antibody (bs-0419R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining