14-3-3 protein zeta/delta antibody

Principal name

14-3-3 protein zeta/delta antibody

Alternative names for 14-3-3 protein zeta/delta antibody

YWHAZ, KCIP-1, Protein kinase C inhibitor protein 1

SwissProt ID

P29361 (Sheep), P63101 (Mouse), P63102 (Rat), P63103 (Bovin), P63104 (Human), Q5ZKC9 (Chick), Q91896 (Xenla)

Gene ID

7534 (YWHAZ)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Sh (Sheep), Can (Canine), Dros (Drosophila), Por (Porcine), African clawed frog, Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)

Background of 14-3-3 protein zeta/delta antibody

It is a member of the 14-3-3 family which consists of 30kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha.

General readings

1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

14-3-3 protein zeta/delta antibody

Immunohistochemistry (IHC) analyzes of 14-3-3 ?/? antibody in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta (134-149) antibody

Human breast ca. stained with Anti-14-3-3 zeta, cat# AP15332PU Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta (134-149) antibody

Human breast ca. stained with Anti-14-3-3 zeta, cat# AP15332PU Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta (134-149) antibody

Human breast ca. stained with Anti-14-3-3 zeta, cat# AP15332PU Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta antibody

Western blot analysis of extracts from K562 cells, using 14-3-3 ?/d (Ab-232) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta antibody

Western blot analysis of extracts from 3T3 cells, using 14-3-3 ß/? (Ab-184/186) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta antibody

Western blot analysis of extracts from HeLa cells, Jurkat cells and COLO cells, using 14-3-3 ? antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta antibody

Western blot analysis of extracts from 293 cells treated with Forskolin (40nM, 30min) using 14-3-3 ? (Ab-58) antibody (#TA314681, Line1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta antibody

Predicted band size: 28 kDa . Positive control: HepG2, Hela and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: HepG2, Hela and 293 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta antibody

Western blot analysis of 14-3-3 zeta expression in Forskolin treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta antibody

Western blot analysis of 14-3-3 zeta/delta expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta antibody

Western blot analysis on RAW264.7 cell lysate using 14-3-3 beta/? Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta antibody

Western blot analysis on NIH-3T3 cell lysate using 14-3-3 ? Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

14-3-3 protein zeta/delta pSer58 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta (Phospho-Ser58) antibody AP02457PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta pSer58 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta (Phospho-Ser58) antibody AP02457PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta antibody AP02701PU. Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta antibody AP02701PU. Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €230.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta (N-term acetylated) antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit IgG Serum Bov, Chk, Hu, Ms, Rt, Sh E, P, WB
0.1 ml / €470.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta pSer58 antibody

Lung, respiratory epithelium: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

14-3-3 protein zeta/delta antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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