14-3-3 protein zeta/delta antibody
Principal name
14-3-3 protein zeta/delta antibody
Alternative names for 14-3-3 protein zeta/delta antibody
YWHAZ, KCIP-1, Protein kinase C inhibitor protein 1
SwissProt ID
P29361 (Sheep), P63101 (Mouse), P63102 (Rat), P63103 (Bovin), P63104 (Human), Q5ZKC9 (Chick), Q91896 (Xenla)
Gene ID
7534 (YWHAZ)
Ncbi ID
NP_001129171.1, 9606, 9940, NP_003397, NP_001129171, NP_001129172, NP_001129173, NP_001129174
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)
Background of 14-3-3 protein zeta/delta antibody
It is a member of the 14-3-3 family which consists of 30kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha.
General readings
1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843.
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta (Phospho-Ser58) antibody AP02457PU.
Figure 3. Immunofluorescence staining of methanol-fixed Hela cells using using 14-3-3 zeta (Phospho-Ser58) antibody AP02457PU.
Figure 2. Western blot analysis of extract from NIH/3T3 cells, untreated or treated with TNF-alpha (20 ng/ml, 5min), using 14-3-3 zeta antibody (lane 1 and 2) and 14-3-3 zeta (Phospho-Ser58) antibody AP02457PU (lane 3 and 4).
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta antibody AP02701PU.
Figure 2. Western blot analysis of extract from NIH/3T3 cells, untreated or treated with TNF-α (20 ng/ml, 5 min), using 14-3-3ζ antibody AP02701PU (lane 1 and 2) and 14-3-3ζ (Phospho-Ser58) antibody AP02457PU (lane 3 and 4).
Immunohistochemistry: 14-3-3 zeta Phosphospecific Antibody [NB100-81766] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 æ (Phospho-Ser58) antibody
Immunofluorescence: 14-3-3 zeta [p Ser58] Antibody [NB100-81766] - Immunofluorescence staining of mehtanol-fixed HEla cells using 14-3-3 zeta (p Ser58) antibody.
Western Blot: 14-3-3 zeta Phosphospecific Antibody [NB100-81766] - Western blot analysis of extract from NIH/3T3 cells, untreated or treated with TNF-a, using 14-3-3 z antibody (Lanes 1 and 2) and 14-3-3 z antibody (Lanes 3 and 4).
Immunohistochemistry (IHC) analyzes of 14-3-3 ζ/δ antibody in paraffin-embedded human colon carcinoma tissue.
Western blot (WB) analysis of 14-3-3 δ antibody (Cat.-No.: AP06623PU-N) at 1/500 dilution
Lane1:MCF-7 cell lysate
Lane2:Hela cell lysate
Lane3:sp2/0 cell lysate
Lane4:H9C2 cell lysate
Lane1:MCF-7 cell lysate
Lane2:Hela cell lysate
Lane3:sp2/0 cell lysate
Lane4:H9C2 cell lysate
Lung, respiratory epithelium: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.Lane 1: anti-gamma 14-3-3 (AP05567SU-N)Lane 2: anti-zeta 14-3-3 (AP05572SU-N)Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)Lane 5: anti-eta 14-3-3. (AP05566SU-N)The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with YWHAZ polyclonal antibody ( Cat # PAB4027 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The YWHAZ polyclonal antibody ( Cat # PAB4027 ) is used in Western blot to detect YWHAZ in Jurkat cell lysate.
Western blot analysis of YWHAZ polyclonal antibody ( Cat # PAB4028 ) in A-375 cell lysate ( 35 µg/lane ) . YWHAZ ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Human HepG2; WB Suggested Anti-YWHAZ Antibody Titration: 5.0ug/ml. Positive Control: HepG2 cell lysate; YWHAZ antibody - C-terminal region (ARP48046_T100) in Human HepG2 cells using Western Blot
Recombinant human 14-3-3 zeta: 15% SDS-PAGE
Formalin-fixed and paraffin-embedded human testis tissue reacted with PLD1 antibody (N-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-14-3-3 protein zeta/delta Anbtibody (T232) in A375 cell line lysates (35ug/lane). 14-3-3 (arrow) was detected using the purified Pab (1:60dilution).
Human breast ca. stained with Anti-14-3-3 zeta, cat# AP15332PU
Western Blot analysis of 293T cell lysate with 14-3-3-zeta antibody
Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunohistochemical staining of YWHAZ on formalin fixed, paraffin embedded human breast carcinoma with YWHAZ polyclonal antibody.
Western Blot: 14-3-3 zeta/delta Lysate [NBL1-17952] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for YWHAZ.
Immunohistochemistry: 14-3-3 zeta Antibody [NB100-91646] - Immunohistochemistry (IHC) analyzes of 14-3-3 zeta (V52) antibody in paraffin-embedded human breast carcinoma tissue.
Western Blot: 14-3-3 zeta Antibody [NB100-91646] - Western blot (WB) analyzes of 14-3-3 zeta (V52) antibody in extracts from 293 cells.
Immunohistochemistry: 14-3-3 zeta/delta Antibody [NB100-92268] - Immunohistochemistry (IHC) analyzes of 14-3-3 æ/ä (W228) antibody in paraffin-embedded human colon carcinoma tissue.
Western Blot: 14-3-3 zeta/delta Antibody [NB100-92268] - Western blot (WB) analysis of 14-3-3 æ/ä (W228) antibody in extracts from K562 cells.
Immunohistochemistry: 14-3-3 zeta Antibody [NBP1-19623] - 14-3-3 zeta Antibody [NBP1-19623] - paraffin-embedded human brain tissue.
Western Blot: 14-3-3 zeta Antibody [NBP1-19623] - extracts from HeLa cells.
14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. 14-3-3 zeta antibody (GTX101075) dilution: 1:500.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using 14-3-3 zeta(GTX101075) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using 14-3-3 zeta (GTX101075) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate) A:NIH-3T312% SDS PAGE GTX101075 diluted at 1:1000
Sample(30 μg of whole cell lysate)A:Hep G2(GTX27900)B:MOLT4(GTX27912)12% SDS PAGEGTX101075 diluted at 1:2000
Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14881-1-AP(YWHAZ antibody) at dilution of 1:400
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14881-1-AP( YWHAZ Antibody) at dilution of 1:1000
Raji cells were subjected to SDS PAGE followed by western blot with 14881-1-AP( YWHAZ Antibody) at dilution of 1:1000
Jurkat cells were subjected to SDS PAGE followed by western blot with 14881-1-AP( YWHAZ Antibody) at dilution of 1:1000
Immunofluorescence: 14-3-3 zeta/delta Antibody [NBP1-31325] - Immunofluorescence analysis of HeLaS3, using 14-3-3 zeta/delta antibody at 1:100 dilution.
Western Blot: 14-3-3 zeta/delta Antibody [NBP1-31325] - Western blot analysis of 14-3-3 zeta/delta Antibody on: A:Hep G2 B:MOLT4 12% SDS PAGE diluted at 1:2000
Western Blot analysis of YWHAZ expression in transfected 293T cell line by YWHAZ monoclonal antibody (M04), clone 1B3.Lane 1: YWHAZ transfected lysate(27.7 KDa).Lane 2: Non-transfected lysate.
Human Liver; Liver; YWHAZ antibody - middle region (ARP48047_P050) in Human Liver cells using Immunohistochemistry
Human Lung; WB Suggested Anti-YWHAZ Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung; YWHAZ antibody - middle region (ARP48047_P050) in Human Lung cells using Western Blot
Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (pVal52) antibody (Cat.-No AP20192PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of 14-3-3 δ antibody (Cat.-No AP20192PU-N) at 1/500 dilution
Lane1:Hela cell lysate
Lane2:Mouse brain tissue lysate
Lane3:Rat kidney tissue lysate
Lane1:Hela cell lysate
Lane2:Mouse brain tissue lysate
Lane3:Rat kidney tissue lysate
Immunohistochemistry (IHC) analyzes of 14-3-3 zeta antibody (Cat.-No AP20468PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of 14-3-3 zeta antibody (Cat.-No AP20468PU-N) in extracts from HeLa cells.
Western Blot: 14-3-3 zeta/delta Antibody (EPR6379) [NBP1-95196] (A) HeLa, (B) SH-SY5Y, (C) Raji, (D) HepG2, (E) C6, (F) Raw264.7, (G) PC-12, and (H) NIH/3T3 cell lysates using anti-14-3-3 zeta/delta.
Western Blot: 14-3-3 Antibody (EPR6380) [NBP1-95238] (A) HeLa, (B) SH-SY5Y, (C) Raji, and (D) HepG2 cell lysates using anti-14-3-3.
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 12% SDS PAGE GTX114140 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta Antibody (GTX50519) (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HepG2 , Hela and 293 cells using 14-3-3z (Ab-58) Antibody GTX50519.
Western blot analysis of extracts from HepG2 , Hela and 293 cells using 14-3-3 zeta Antibody (GTX50519).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58) Antibody (GTX50712) (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed Hela cells using 14-3-3 zeta (Phospho-Ser58) Antibody (GTX50712).
Western blot analysis of extracts from Hela cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58) Antibody (GTX50712).
Mouse splenocytes probed with Rabbit Anti-14-3-3 zeta delta Polyclonal Antibody, FITC Conjugated (bs-6790R-FITC) at 1:100 for 40 minutes(green)compared to control cells (blue).
Antigen: bs-9654P, 2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; TMB stainingRead the data in Microplate Reader by 450nm.
Western blot analysis of extracts from Jurkat cells treated with UV using Phospho-14-3-3 zeta/ delta (Thr232) antibody AP55906PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of 14-3-3 zeta (pS58) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of 14-3-3 zeta (pS58) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of 14-3-3 zeta (pS58) expression in MCF7 (A); A549 (B); SP2/0 (C); rat liver (D); NIH3T3 (E); A431 (F) whole cell lysates.
Immunohistochemical analysis of 14-3-3 zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of 14-3-3 zeta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of 14-3-3 zeta expression in HeLa (A); mouse brain (B); rat kidney (C) whole cell lysates.
Western blot analysis of 14-3-3 zeta expression in KNRK (A); NIH3T3 (B); C4 (C); EOC20 (D); CTLL2 (E) whole cell lysates.
Immunohistochemical analysis of 14-3-3 zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of 14-3-3 zeta staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of 14-3-3 zeta expression in MCF7 (A); HeLa (B); SP2/0 (C); H9C2 (D) whole cell lysates.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP06623PU-N | 14-3-3 protein zeta/delta antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€350.00
|
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| Acris Antibodies GmbH | |||||||
 +1 additional image |
Published recently! |
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| AP15332PU-M | 14-3-3 protein zeta/delta (134-149) antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | P, WB |
0.5 ml /
€360.00
|
||
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
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| AP15332PU-N | 14-3-3 protein zeta/delta (134-149) antibody |
||||||
|
Rabbit | Purified | Hu, Ms, Rt | P, WB |
1 ml /
€520.00
|
||
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
||||||
| AP15332PU-S | 14-3-3 protein zeta/delta (134-149) antibody |
||||||
|
Rabbit | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€190.00
|
||
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
||||||
| TA311637 | 14-3-3 protein zeta/delta antibody |
||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA314677 | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA314678 | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA314681 | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, IP, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA323870 | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA325982 | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325983 | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347331 | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347722 | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP02457PU-N | 14-3-3 protein zeta/delta pSer58 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€320.00
|
||
| Acris Antibodies GmbH | |||||||
| +2 additional images |
|||||||
| AP02457PU-S | 14-3-3 protein zeta/delta pSer58 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
50 µl /
€240.00
|
||
| Acris Antibodies GmbH | |||||||
| +2 additional images |
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| AP02701PU-N | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 ml /
€270.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
|||||||
| AP02701PU-S | 14-3-3 protein zeta/delta antibody |
||||||
|
Rabbit | Aff - Purified | Hu, Ms | P, WB |
50 µl /
€230.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP05572SU-N | 14-3-3 protein zeta/delta (N-term acetylated) antibody |
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 |
Rabbit | IgG | Serum | Bov, Chk, Hu, Ms, Rt, Sh | E, P, WB |
0.1 ml /
€470.00
|
|
| Acris Antibodies GmbH | |||||||
| AP07437PU-N | 14-3-3 protein zeta/delta pSer58 antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | P, WB |
50 µg /
€470.00
|
|
| Acris Antibodies GmbH | |||||||
| AP08195PU-N | 14-3-3 protein zeta/delta antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | P, WB |
50 µg /
€470.00
|
|
| Acris Antibodies GmbH | |||||||
