14-3-3 protein zeta/delta antibody

Principal name

14-3-3 protein zeta/delta antibody

Alternative names for 14-3-3 protein zeta/delta antibody

YWHAZ, KCIP-1, Protein kinase C inhibitor protein 1

Selected pictures

14-3-3 protein zeta/delta antibody - A0001-1 14-3-3 protein zeta/delta
A0001-1 14-3-3 protein zeta/delta - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is treated with the synthesized peptide.
14-3-3 protein zeta/delta antibody - A0001-1 14-3-3 protein zeta/delta
A0001-1 14-3-3 protein zeta/delta - Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is treated with the synthesized peptide.

SwissProt ID

P29361 (Sheep), P63101 (Mouse), P63102 (Rat), P63103 (Bovin), P63104 (Human), Q5ZKC9 (Chick), Q91896 (Xenla)

Gene ID

7534 (YWHAZ), 25578 (Ywhaz)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Sh (Sheep), Dros (Drosophila), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)

Background of 14-3-3 protein zeta/delta antibody

It is a member of the 14-3-3 family which consists of 30kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha.

General readings

1. Simeone P  et al. A unique four-hub protein cluster associates to glioblastoma progression.PLoS One 9:e103030 (2014). IHC-P ; Human .PubMed: 25050814
2. Ota S  et al. Contribution of fetal ANXA5 gene promoter polymorphisms to the onset of pre-eclampsia. Placenta 34:1202-10 (2013). Human .PubMed: 24140079
3. Deng MY  et al. Frontal-Subcortical Protein Expression following Prenatal Exposure to Maternal Inflammation. PLoS One 6:e16638 (2011). WB ; Mouse .PubMed: 21347362
4. Broadstock M  et al. Synaptic protein expression is regulated by a pro-oxidant diet in APPxPS1 mice. J Neural Transm : (2011). WB ; Mouse .PubMed: 22057511
5. Koseki N  et al. 17ß-estradiol prevents reduction of retinal phosphorylated 14-3-3 zeta protein levels following a neurotoxic insult. Brain Res : (2011). WB, ICC/IF, IHC-P ; Rat .PubMed: 22154405
6. Bansal A  et al. Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction. J Proteomics 73:2041-9 (2010). WB ; Rat .PubMed: 20601275

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
GTX50519

14-3-3 protein zeta/delta antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta Antibody (GTX50519) (left) or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt C, WB
0.1 ml / €370.00
  GeneTex Inc.
GTX50712

14-3-3 protein zeta/delta antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58) Antibody (GTX50712) (left) or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €370.00
  GeneTex Inc.
GTX63231

14-3-3 protein zeta/delta antibody

Western blot analysis on (A) HeLa, (B) SH-SY5Y, (C) Raji, (D) HepG2, (E) C6, (F) Raw264.7, (G) PC-12, and (H) NIH/3T3 cell lysates using anti-14-3-3 zeta/delta RabMAb (cat. #GTX63231). Rabbit IgG EPR6379 Hu, Ms, Rt WB
0.1 ml / €420.00
  GeneTex Inc.
GTX63289

14-3-3 protein zeta/delta antibody

WB analysis of (1) HeLa, (2) SH-SY5Y, (3) Raji and (4) HepG2 lysates (10 μg per lane) using 14-3-3 antibody [EPR6380] at a dilution of 1:1,000. Rabbit IgG EPR6380 Hu, Ms, Rt WB
0.1 ml / €420.00
  GeneTex Inc.
GTX79155

14-3-3 protein zeta/delta antibody

14-3-3 protein zeta/delta Rabbit IgG Aff - Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 mg / €430.00
  GeneTex Inc.
GTX114140

14-3-3 protein zeta/delta antibody

Sample (30 ug of whole cell lysate)  A: NIH-3T3   B: JC   12% SDS PAGE  GTX114140 diluted at 1:1000 Rabbit IgG Aff - Purified Ms WB
0.1 ml / €350.00
  GeneTex Inc.
H00007534-B02P

14-3-3 protein zeta/delta antibody

14-3-3 protein zeta/delta Mouse Purified Hu E(detection), WB
50 µg / €360.00
  Abnova Taiwan Corp.
H00007534-M04

14-3-3 protein zeta/delta antibody

Western Blot analysis of YWHAZ expression in transfected 293T cell line by YWHAZ monoclonal antibody (M04), clone 1B3.

Lane 1: YWHAZ transfected lysate(27.7 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 1B3 Purified Hu E, WB
0.1 mg / €360.00
  Abnova Taiwan Corp.
H00007534-M12

14-3-3 protein zeta/delta antibody

14-3-3 protein zeta/delta Mouse IgG2a 5E8 Purified Hu E, WB
0.1 mg / €360.00
  Abnova Taiwan Corp.
PAB4027

14-3-3 protein zeta/delta antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with YWHAZ polyclonal antibody ( Cat # PAB4027 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.
PAB4028

14-3-3 protein zeta/delta antibody

Western blot analysis of YWHAZ polyclonal antibody ( Cat # PAB4028 ) in A-375 cell lysate ( 35 µg/lane ) . YWHAZ ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.
PAB11083

14-3-3 protein zeta/delta antibody

Immunohistochemical staining of YWHAZ on formalin fixed, paraffin embedded human breast carcinoma with YWHAZ polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.
500-12604

14-3-3 protein zeta/delta antibody

14-3-3 protein zeta/delta Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €250.00
  AbboMax Inc
A0001-1

14-3-3 protein zeta/delta pSer58 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech
A0001-2

14-3-3 protein zeta/delta pSer58 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech
A0759-1

14-3-3 protein zeta/delta pThr232 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech
A0759-2

14-3-3 protein zeta/delta pThr232 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech
A8001-1

14-3-3 protein zeta/delta pSer184/186 antibody

Western blot analysis of extracts from RAW264.7 cells treated with UV 15', using 14-3-3 beta/zeta (Phospho-Ser184/186) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech
A8001-2

14-3-3 protein zeta/delta pSer184/186 antibody

Western blot analysis of extracts from RAW264.7 cells treated with UV 15', using 14-3-3 beta/zeta (Phospho-Ser184/186) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech
B0001-1

14-3-3 protein zeta/delta antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta (Ab-58) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
50 µg / €205.00
  Assay Biotech

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