Home » Target » 17-beta-HSD1 / HSD17B1 antibody

17-beta-HSD1 / HSD17B1 antibody

Principal name

17-beta-HSD1 / HSD17B1 antibody

Alternative names for 17-beta-HSD1 / HSD17B1 antibody

20 alpha-hydroxysteroid dehydrogenase, 20-alpha HSD, 17E2DH, EDH17B1, EDH17B2, EDHB17, E17KSR, Estradiol 17-beta-dehydrogenase 1, 17-beta-hydroxysteroid dehydrogenase type 1, Placental 17-beta-hydroxysteroid dehydrogenase

SwissProt ID

P14061 (Human), P51656 (Mouse), P51657 (Rat)

Gene ID

3292 (HSD17B1), 25322 (Hsd17b1)

Ncbi ID

9606, NP_000404, NP_000404.2, NP_036983.1

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of 17-beta-HSD1 / HSD17B1 antibody

The principal human estrogen, 17 beta-estradiol, is a potent stimulator of certain endocrine-dependent forms of breast cancer. Because human estrogenic 17 beta-hydroxysteroid dehydrogenase (HSD17B1) catalyzes the last step in the biosynthesis of 17 beta-estradiol from the less potent estrogen, estrone, it is an attractive target for the design of inhibitors of estrogen production and tumor growth (1). It is concluded that the steroid-binding site of human placental HSD17B1 contains a histidine residue which proximates the upper A-ring region of the steroid as it undergoes the reversible binding step (2). Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies (3). Defects in the conversion of androstenedione to testosterone in the fetal testes by the enzyme HSD17B1 give rise to genetic males with female external genitalia (4)

General readings

Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Lamp, M., et al. Gynecol. Endocrinol. (2010) In press
Haller, F., et al. J. Mol. Biol. 399(2):255-267(2010)
Iwasaki, M., et al. Nutr Cancer 62(4):466-475(2010)

Immunoperoxidase of monoclonal antibody to HSD17B1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

H00003292-M03

HSD17B1 monoclonal antibody (M03), clone 2E5 Western Blot analysis of HSD17B1 expression in Jurkat ( Cat # L017V1 ).

H00003292-M03

Immunohistochemistry: HSD17B1 (C-term) Antibody (EP1682Y) [NB110-57057] - Immunohistochemical staining of paraffin-embedded human placenta using anti-HSD17B1 (cat. #110-57057).

NB110-57057

Western Blot: HSD17B1 (C-term) Antibody (EP1682Y) [NB110-57057] - Western blot analysis on human placenta lysate using anti-HSD17B1 (cat. # 110-57057), dilution 1:100,000.

NB110-57057

HSD17B1 monoclonal antibody (M03), clone 2E5 Western Blot analysis of HSD17B1 expression in Jurkat ( Cat # L017V1 ).

H00003292-M03A

Human Kidney; Rabbit Anti-HSD17B1 Antibody. Catalog Number: ARP41727. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; HSD17B1 antibody - N-terminal region (ARP41727_P050) in Human Kidney cells using Immunohistochemistry

ARP41727_P050

Human Jurkat; WB Suggested Anti-HSD17B1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; HSD17B1 antibody - N-terminal region (ARP41727_P050) in Human Jurkat cells using Western Blot

ARP41727_P050

Western Blot: HSD17B1 Antibody [NBP1-56295] - This Anti-HSD17B1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 0.5ug/ml.

NBP1-56295

Western blot analysis of HSD17B1 Antibody (C-term) Cat.-No AP52108PU-N in SK-BR-3 cell line lysates (35ug/lane). This demonstrates the HSD17B1 antibody detected the HSD17B1 protein (arrow).

AP52108PU-N

Immunohistochemistry-Paraffin: HSD17B1 Antibody [NBP1-84901] Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in Syncytiotrophoblasts.

NBP1-84901

A. Western blot analysis on human placenta lysate using anti-HSD17B1 RabMAb (cat. # GTX61596) dilution 1:100000.

GTX61596

B. Immunohistochemical staining of paraffin-embedded human placenta using anti-HSD17B1 RabMAb (cat. #GTX61596).

GTX61596

A. Western blot analysis on human placenta lysate using anti-HSD17B1 RabMAb (cat. # GTX61715) dilution 1:500.

GTX61715

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-HSD17B1 Polyclonal Antibody, Unconjugated (bs-3855R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

bs-3855R

Lane 1: mouse liver lysates Lane 2: mouse intestine lysates probed with Anti HSD17B1/HSD17 Polyclonal Antibody, Unconjugated (bs-6603R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 36kD. Observed band size: 36kD

bs-6603R

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AP52108PU-N	17-beta-HSD1 / HSD17B1 (C-term) antibody
	Rabbit	Ig		Aff - Purified	Hu	WB	0.4 ml / €370.00
							Acris Antibodies GmbH
H00003292-M03	17-beta-HSD1 / HSD17B1 antibody
	Mouse	IgG1	2E5	Purified	Hu	E, P, WB	0.1 mg / €370.00
							Abnova Taiwan Corp.
+1 additional image
H00003292-M03A	17-beta-HSD1 / HSD17B1 antibody
	Mouse	IgG1	2E5	Ascites	Hu	E, WB	0.2 ml / €370.00
							Abnova Taiwan Corp.
ARP41727_P050	17-beta-HSD1 / HSD17B1 antibody
	Rabbit			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	P, WB	50 µg / €350.00
							AVIVA Systems Biology
+1 additional image
BB-PA1613	17-beta-HSD1 / HSD17B1 antibody
	Rabbit	IgG		Aff - Purified	Ms, Rt	WB	0.1 mg / €290.00
							Boster Immunoleader
NB110-57057	17-beta-HSD1 / HSD17B1 (C-term) antibody
	Rabbit	IgG	EP1682Y	Purified	Hu	F, ICC/IF, IP, P, WB	0.1 ml / €480.00
							Novus Biologicals Inc.
+1 additional image
NBP1-56295	17-beta-HSD1 / HSD17B1 antibody
	Rabbit			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	P, WB	50 µg / €440.00
							Novus Biologicals Inc.
NBP1-84901	17-beta-HSD1 / HSD17B1 antibody
	Rabbit	IgG		Aff - Purified	Hu	P	0.1 ml / €500.00
							Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
AR50925PU-N	17-beta-HSD1 / HSD17B1 (1-328, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.25 mg / €730.00
					Acris Antibodies GmbH
AR50925PU-S	17-beta-HSD1 / HSD17B1 (1-328, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	50 µg / €300.00
					Acris Antibodies GmbH
H00003292-Q01-10	17-beta-HSD1 / HSD17B1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.	10 µg / €380.00
					Abnova Taiwan Corp.
H00003292-Q01-25	17-beta-HSD1 / HSD17B1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.	25 µg / €510.00
					Abnova Taiwan Corp.

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products