58K Golgi Protein antibody

Principal name

58K Golgi Protein antibody

Alternative names for 58K Golgi Protein antibody

FTCD, LCHC1, Golgi marker, Formimidoyltransferase-cyclodeaminase, Golgi Marker 58K, Formiminotransferase-cyclodeaminase

SwissProt ID

O88618 (Rat), O95954 (Human), P53603 (Pig), Q91XD4 (Mouse), Q9YH58 (Chick)

Gene ID

10841 (FTCD)

Available reactivities

Bov (Bovine), Can (Canine), Hst (Hamster), Hu (Human), Por (Porcine), Rt (Rat), Ms (Mouse), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Goat, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Flow Cytometry (F), Luminex (LMNX)

Background of 58K Golgi Protein antibody

The 58K Golgi protein is one of the most commonly used Golgi markers and has been proposed to implicate in linking Golgi to microtubules and it peripherally associates with cytosolic surface of Golgi membranes. This protein shows extensive overall sequence identity with porcine FTCD and has been suggested to represent rat FTCD (formiminotransferase-cyclodeamise), a metabolic enzyme involved in the conversion of histidine to glutamic acid. FTCD's formiminotransferase activity facilitate transfer of a a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the cyclodeamise activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. FTCD appears to be a dymic component of Golgi and cycles between Golgi and earlier compartments of the secretory pathway. It also binds and promotes bundling of vimentin filaments origiting from the Golgi, and mutations in this gene encoding for 58K Golgi Protein i.e. FTCD are associated with glutamate formiminotransferase deficiency (FIGLU-URIA).

General readings

Bloom GS & Brashear TA A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem 264:16083-92 (1989). PubMed PMID: 2777777

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

58K Golgi Protein (N-term) antibody

TA302953 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Por E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

58K Golgi Protein (N-term) antibody

TA302953 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Por E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

58K Golgi Protein antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FTCDmouse monoclonal antibody. (UM500087; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB122 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

58K Golgi Protein antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FTCD mouse monoclonal antibody. (UM500088; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB123 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

58K Golgi Protein antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FTCDmouse monoclonal antibody. (UM500087; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB122 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

58K Golgi Protein antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FTCD mouse monoclonal antibody. (UM500088; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB123 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

58K Golgi Protein antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FTCD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504944) Mouse IgG1 OTI1A7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

58K Golgi Protein antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FTCD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504951) Mouse IgG1 OTI4F5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

58K Golgi Protein antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FTCD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505028) Mouse IgG2b OTI4A7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

58K Golgi Protein antibody

Immunoperoxidase of monoclonal antibody to FTCD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml] Mouse IgG2a 3A4 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

58K Golgi Protein antibody

TA302954 staining (0.03µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

58K Golgi Protein antibody

TA302954 staining (0.03µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

58K Golgi Protein antibody

Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308819 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

58K Golgi Protein antibody

Western Blot: 58K Golgi Protein Antibody (58K-9) [TA336687] - Analysis of 58K golgi protein expression in rat liver tissue using TA336687. Mouse IgG1 58K-9 Purified Bov, Can, Chk, Hu, Ms, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

58K Golgi Protein antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FTCD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG1 OTI7C5 Purified Hu, Mky F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

58K Golgi Protein antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FTCD (RC208573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FTCD. Mouse IgG1 OTI4C3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

58K Golgi Protein antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FTCD (RC208573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FTCD. Mouse IgG1 OTI4A1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

58K Golgi Protein antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FTCD (RC208573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FTCD. Mouse IgG1 OTI4B6 Purified Hu, Mky, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

58K Golgi Protein antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FTCD (RC208573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FTCD. Mouse IgG1 OTI2B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

58K Golgi Protein antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FTCD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504981) Mouse IgG2a OTI8C2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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