6CKine / CCL21 antibody

Principal name

6CKine / CCL21 antibody

Alternative names for 6CKine / CCL21 antibody

Small-inducible cytokine A21, Beta chemokine Exodus-2, SCYA21, CCL-21, SLC, Secondary lymphoid-tissue chemokine

SwissProt ID

O00585 (Human)

Gene ID

6366 (CCL21)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Neutralisation (NEUT), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of 6CKine / CCL21 antibody

Mouse 6Ckine is a beta- or C-C chemokine identified in the Expressed Sequence Tag (EST) database by three independent groups (Hedrick and Zlotnik, Hromas et al, Nagira et al). Known also as Exodus-2 and secondary lymphoid-tissue chemokine (SLC), 6Ckine contains four conserved cysteine (C) residues which are characteristic of beta-chemokines (Hedrick and Zlotnik, Hromas et al, Nagira et al). Two additional conserved cysteine residues have been found in its unusually long carboxy-terminal domain and consequently the name 6Ckine (Hedrick and Zlotnik, Hromas et al, Nagira et al). Human and mouse 6Ckine are highly conserved and show 86% amino acid homology.1 Mouse 6Ckine cDNA encodes a 133 amino acid precursor protein, a 23 amino acid signal peptide and a 110 amino acid mature protein. (Hedrick and Zlotnik). Comparatively, human 6Ckine cDNA encodes a 134 amino acid precursor protein, a 23 amino acid signal peptide and a 111 amino acid mature protein(Hedrick and Zlotnik, Nagira et al). Recombinant mouse 6Ckine has a predicted molecular mass of approximately 12 kDa (mature protein). In SDS-PAGE under reducing and non-reducing conditions, the recombinant protein migrates with an apparent molecular mass of 16 kDa to 17 kDA.The expression of mouse 6Ckine has been detected in high levels in the spleen and lung (Nagira et al). Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells (Hedrick and Zlotnik). Unlike other C-C chemokines, 6Ckine is not chemotactic for monocytes and neutrophils (Hedrick and Zlotnik, Nagira et al). A growing body of work suggests that 6Ckine influences lymphocyte homing to secondary lymphoid organs (Gunn et al), integrin-mediated lymphocyte adhesion (Pachynski et al) and may act via the EBI1 ligand chemokine (ELC) receptor, CCR7 (Willimann et al, Yoshida et al).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

6CKine / CCL21 antibody


Staining of Human Lymph Node lysate using Cat.-No AP32059PU-N CCL21 Antibody 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

6CKine / CCL21 antibody

To detect hExodus-2 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hExodus-2. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

6CKine / CCL21 antibody

To detect hExodus-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Exodus-2 (TA328305) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hExodus-2. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

6CKine / CCL21 (Center) antibody

CCL21 Antibody (Center) (Cat. #AP50801PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CCL21 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

6CKine / CCL21 antibody

6CKine / CCL21 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

6CKine / CCL21 antibody

6CKine / CCL21 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

6CKine / CCL21 antibody

6CKine / CCL21 Rabbit Aff - Purified Hu E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

6CKine / CCL21 antibody

6CKine / CCL21 Rabbit Aff - Purified Hu E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

6CKine / CCL21 antibody

Western Blot analysis of CCL21 expression in transfected 293T cell line (H00006366-T01) by CCL21 MaxPab polyclonal antibody.

Lane 1: CCL21 transfected lysate(14.74 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

6CKine / CCL21 antibody

Western Blot analysis of CCL21 expression in transfected 293T cell line (H00006366-T01) by CCL21 MaxPab polyclonal antibody.

Lane 1: CCL21 transfected lysate(14.74 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

6CKine / CCL21 antibody

Immunoprecipitation of CCL21 transfected lysate using anti-CCL21 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CCL21 MaxPab mouse polyclonal antibody (B01) (H00006366-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

6CKine / CCL21 antibody

Western Blot analysis of CCL21 expression in transfected 293T cell line (H00006366-T01) by CCL21 MaxPab polyclonal antibody.

Lane 1: CCL21 transfected lysate(14.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

6CKine / CCL21 antibody

6CKine / CCL21 Rabbit Purified Rt WB
0.2 mg / €290.00
  Torrey Pines Biolabs

6CKine / CCL21 antibody

6CKine / CCL21 Rabbit Azide Free WB
0.2 mg / €290.00
  Torrey Pines Biolabs

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

6CKine / CCL21

6CKine / CCL21 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

6CKine / CCL21

6CKine / CCL21 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 20 µg / €199.00

Special Price: 20 µg / €139.00

  OriGene Technologies, Inc.

6CKine / CCL21

6CKine / CCL21 Human > 98 %
Preparation: .
Purity Detail: >98%, as determined by Coomassie stained SDS-PAGE.
E. coli

Regular Price: 10 µg / €119.00

Special Price: 10 µg / €99.00

  OriGene Technologies, Inc.

6CKine / CCL21 (24-134, His-tag)

Recombinant human CCL21, 24-134 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS-PAGE E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

6CKine / CCL21 (24-134, His-tag)

Recombinant human CCL21, 24-134 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS-PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

6CKine / CCL21

6CKine / CCL21 Human > 98 % pure by SDS-PAGE and HPLC analyses. E. coli
5 µg / €140.00
  Acris Antibodies GmbH

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