AADAC antibody

Principal name

AADAC antibody

Alternative names for AADAC antibody

CES5A1, DAC,arylacetamide deacetylase

SwissProt ID

P22760 (Human), Q99PG0 (Mouse), Q9QZH8 (Rat)

Gene ID

13 (AADAC)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Rb (Rabbit), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of AADAC antibody

The assembly of very-low-density lipoproteins (VLDLs) in the secretory apparatus of the hepatocyte relies on the mobilization of triacylglycerol (TAG) from the cytosolic pool by lipolysis and re-esterification. However, some of the re-esterified TAG products are returned to the cytosolic pool in the liver, which protects vulnerable body tissues from excess lipotoxic non-esterified fatty acids in the plasma. Some of the lipases involved in this process include arylacetamide deacetylase (AADAC) and its related proteins AADACL1 and AADACL2. AADAC, a single pass type II membrane protein of the endoplasmic reticulum, is expressed in hepatocytes, intestinal mucosal cells, pancreas and adrenal gland. It plays an important role in the metabolic activation of arylamine substrates to ultimate carcinogens. AADACL1 hydrolyzes the metabolic intermediate 2-acetyl monoalkylglycerol, and its inactivation results in disruption of ether lipid metabolism in cancer cells and impaired cell migration and tumor growth.

General readings

Frick,C., (2004) J. Biol. Chem. 279 (30), 31131-31138

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AADAC antibody

Predicted band size: 46 kDa . Positive control: Human fetal liver and liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human fetal liver tissue lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AADAC antibody

Predicted band size: 46 kDa . Positive control: Human fetal liver and liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human fetal liver tissue lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AADAC (C-term) antibody

Western blot analysis of AADAC Antibody (C-term) in 293 cell line lysates (35 µg/lane). AADAC (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AADAC antibody

AADAC Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

AADAC antibody

Detection limit for recombinant GST tagged AADAC is approximately 3ng/ml as a capture antibody. Mouse IgG2b 2E8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AADAC antibody

Hum. Fetal Liver; Host:Rabbit. Target Name:AADAC. Sample Tissue:Human Fetal Liver. Antibody Dilution: 1.0ug/ml.; AADAC antibody - N-terminal region (ARP45036_P050) in Hum. Fetal Liver cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rb WB
50 µg / €350.00
  AVIVA Systems Biology

AADAC antibody

Human Liver; WB Suggested Anti-AADAC Antibody Titration: 2.5ug/ml. Positive Control: Human Liver; AADAC antibody - C-terminal region (ARP45038_T100) in Human Liver cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Rb WB
0.1 mg / €310.00
  AVIVA Systems Biology

AADAC antibody

AADAC Rabbit IgG Hu WB
0.1 ml / €340.00
  Novus Biologicals Inc.

AADAC antibody

Western Blot: AADAC Antibody [NBP1-62428] - This Anti-AADAC antibody was used in Western Blot of Transfected 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

AADAC antibody

Western Blot: AADAC Antibody [NBP1-69445] - This Anti-AADAC antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 2.5ug/ml. Rabbit Purified Hu WB
0.1 mg / €360.00
  Novus Biologicals Inc.

AADAC antibody

Immunohistochemistry-Paraffin: AADAC Antibody [NBP1-85680] - Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AADAC

AADAC Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

AADAC

AADAC Human
2 µg / €300.00
  Abnova Taiwan Corp.

AADAC

AADAC Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

AADAC

AADAC Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

AADAC

AADAC Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

AADAC

AADAC Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

AADAC overexpression lysate

AADAC overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

18 Item(s)

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