AAK1 antibody

Principal name

AAK1 antibody

Alternative names for AAK1 antibody

AP2-associated protein kinase 1, KIAA1048

SwissProt ID

P0C1X8 (Rat), Q2M2I8 (Human), Q3UHJ0 (Mouse)

Gene ID

22848 (AAK1), 269774 (Aak1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of AAK1 antibody

AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

General readings

Henderson DM, Conner SD, A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol Biol Cell. 2007 Jul;18(7):2698-706.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AAK1 (N-term) antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AAK1 (C-term) antibody

Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AAK1 (N-term) antibody

Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AAK1 antibody

(1ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AAK1 antibody

Western blot analysis of extracts from COS7 cells, using AAK1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AAK1 antibody

WB Suggested Anti-AAK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that AAK1 is expressed in 721_B Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AAK1 antibody

Western blot analysis on COS7 cell lysate using AAK1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AAK1 (C-term) antibody

Western blot analysis of anti-AAK1 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AAK1 (N-term) antibody

Western blot analysis of AAK1 Antibody (N-term) (AP17859PU-N) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AAK1 (C-term) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AAK1 Control Peptide

AAK1
50 µg / €160.00
  Acris Antibodies GmbH

AAK1 Control Peptide

AAK1
50 µg / €160.00
  Acris Antibodies GmbH

AAK1 antibody

Western Blot analysis of AAK1 expression in transfected 293T cell line (H00022848-T01) by AAK1 MaxPab polyclonal antibody.

Lane 1: AAK1 transfected lysate(52.14 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

AAK1 antibody

Western Blot analysis of AAK1 expression in transfected 293T cell line by AAK1 monoclonal antibody (M02), clone 4B10.

Lane 1: AAK1 transfected lysate(52 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AAK1 antibody

Western blot analysis of AAK1 expression in NIH/3T3 cell lysate ( Lane 1 ) and rat kidney tissue lysate ( Lane 2 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

AAK1 antibody

Western blot analysis of extracts from COS7 cells, using AAK1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

AAK1 antibody

Western blot analysis of extracts from COS7 cells, using AAK1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

AAK1 (C-term) antibody

Mouse Thymus; WB Suggested Anti-Aak1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Thymus; Aak1 antibody - C-terminal region (ARP55101_P050) in Mouse Thymus cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

AAK1 (C-term) antibody

Human 721_B; WB Suggested Anti-AAK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. There is BioGPS gene expression data showing that AAK1 is expressed in 721_B.; AAK1 antibody - C-terminal region (ARP55102_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

AAK1 antibody

AAK1 Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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