AAK1 antibody

Principal name

AAK1 antibody

Alternative names for AAK1 antibody

AP2-associated protein kinase 1, KIAA1048

SwissProt ID

P0C1X8 (Rat), Q2M2I8 (Human), Q3UHJ0 (Mouse)

Gene ID

22848 (AAK1), 269774 (Aak1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of AAK1 antibody

AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

General readings

Henderson DM, Conner SD, A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol Biol Cell. 2007 Jul;18(7):2698-706.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AAK1 (N-term) antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AAK1 (C-term) antibody

  Rabbit Polyclonal Aak1 Antibody  
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AAK1 (N-term) antibody

  Rabbit Polyclonal Aak1 Antibody  
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AAK1 antibody

  Goat Anti-AAK1 Antibody  
(1ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AAK1 antibody

  Rabbit polyclonal anti-AAK1 antibody  
Western blot analysis of extracts from COS7 cells, using AAK1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AAK1 antibody

  Rabbit Polyclonal Anti-AAK1 Antibody  
WB Suggested Anti-AAK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that AAK1 is expressed in 721_B Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AAK1 antibody

  Rabbit Polyclonal Anti-AAK1 Antibody  
Western blot analysis on COS7 cell lysate using AAK1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AAK1 (C-term) antibody

Western blot analysis of anti-AAK1 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AAK1 (N-term) antibody

Western blot analysis of AAK1 Antibody (N-term) (AP17859PU-N) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AAK1 (C-term) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AAK1 antibody

Western blot analysis of extracts from COS7 cells, using AAK1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

AAK1 antibody

Western blot analysis of extracts from COS7 cells, using AAK1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

AAK1 - human gene knockout kit via CRISPR

AAK1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

AAK1 - human gene knockout kit via CRISPR

AAK1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

AAK1 - human gene knockout kit via CRISPR

AAK1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

AAK1 - human gene knockout kit via CRISPR

AAK1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Aak1 - mouse gene knockout kit via CRISPR

Aak1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

AAK1-knockout Cell lysate

AAK1-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, AAK1 (mGFP-tagged)-Human AP2 associated kinase 1 (AAK1), 200 uL, >10^7 TU/mL

Lenti ORF particles, AAK1 (mGFP-tagged)-Human AP2 associated kinase 1 (AAK1), 200 uL, >10^7 TU/mL  
200 µl / €1,980.00
  OriGene Technologies, Inc.

Lenti ORF particles, AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1), 200 uL, >10^7 TU/mL

Lenti ORF particles, AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1), 200 uL, >10^7 TU/mL  
200 µl / €1,780.00
  OriGene Technologies, Inc.

20 Item(s)

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