AATF antibody

Principal name

AATF antibody

Alternative names for AATF antibody

CHE1, DED, HSPC277, CHE-1, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1

SwissProt ID

Q9JKX4 (Mouse), Q9NY61 (Human), Q9QYW0 (Rat)

Gene ID

26574 (AATF)

Available reactivities

Can (Canine), Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Ms (Mouse), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Frozen Sections (C)

Background of AATF antibody

AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK) (1), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases (2). AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways (3). Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the b-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer’s disease (AD) (4,5), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.

General readings

Burgdorf,S., et al., (2004) J. Biol. Chem. 279 (17), 17524-17534

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AATF antibody

Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AATF antibody

Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO205 cells and HUVEC cells, using AATF antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AATF antibody

WB Suggested Anti-AATF Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 312500; Positive Control: Daudi cell lysate. AATF is supported by BioGPS gene expression data to be expressed in Daudi Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AATF antibody

WB Suggested Anti-AATF Antibody Titration: 0.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human kidney Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AATF antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Testis. Rabbit Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AATF Control Peptide

AATF
50 µg / €160.00
  Acris Antibodies GmbH

AATF antibody

AATF polyclonal antibody (A01), Lot # AUH0060323QCS1 Western Blot analysis of AATF expression in PC-12 ( Cat # L012V1 ). Mouse Serum Hu, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

AATF antibody

Immunoperoxidase of monoclonal antibody to AATF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG1 3C7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AATF antibody

Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2H6 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AATF antibody

Immunohistochemistry of AATF in human small intestine tissue with AATF polyclonal antibody ( Cat # PAB12886 ) at 10 µg/ml. Rabbit Hu, Ms P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

AATF antibody

Western blot analysis of extracts from HeLa cells/HepG2 cells/COLO205 cells/HuvEc cells, using AATF Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

AATF antibody

Western blot analysis of extracts from HeLa cells/HepG2 cells/COLO205 cells/HuvEc cells, using AATF Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

AATF antibody

Human Intestine; AATF antibody - N-terminal region (ARP31867_P050) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

AATF antibody

Human Daudi; WB Suggested Anti-AATF Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysate. AATF is supported by BioGPS gene expression data to be expressed in Daudi.; AATF antibody - N-terminal region (ARP38957_T100) in Human Daudi cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

AATF antibody

Human kidney; WB Suggested Anti-AATF Antibody Titration: 0.25ug/ml. ELISA Titer: 1:312500. Positive Control: Human kidney; AATF antibody - C-terminal region (ARP38958_T100) in Human kidney cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

AATF antibody

Immunohistochemical analysis of AATF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AATF antibody

Immunohistochemical analysis of AATF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AATF antibody

Immunohistochemical analysis of AATF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AATF antibody

Western Blot and Immunoprecipitation: AATF/Che-1 Antibody [NB100-55324] - Detection of Human AATF/Che-1 on HeLa whole cell lysates using NB100-55324. AATF/Che-1 was also immunoprecipitated by rabbit anti-AATF/Che-1 antibody NB100-55325. Rabbit Aff - Purified Hu IP, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

AATF antibody

Immunohistochemistry-Paraffin: AATF Antibody [NBP1-76655] - AATF in human small intestine tissue with AATF antibody at 10 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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