ABCA1 antibody

Principal name

ABCA1 antibody

Alternative names for ABCA1 antibody

ABC1, ABC-1, CERP, ATP-binding cassette sub-family A member 1

SwissProt ID

O95477 (Human), P41233 (Mouse)

Gene ID

19 (ABCA1), 11303

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Can (Canine), Chk (Chicken), Hst (Hamster), Por (Porcine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ChIP assay (ChIP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of ABCA1 antibody

ABCA1 (ATP-binding cassette, sub-family A (ABC1), member 1), also known as ABC1, the cholesterol efflux regulatory protein (CERP), is a protein which in humans is encoded by the ABCA1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes,contains 2,261 amino acids. Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, with highest expression in placenta, liver, lung, adrenal glands, and all fetal tissues examined, and lowest expression in kidney, pancreas, pituitary, mammary gland, and bone marrow. This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterolefflux  pump in the cellular lipid removal pathway. Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, Szakacs et al. found specific, Mg(2+)-dependent ATP binding and low basal ATPase activity. Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1. Szakacs et al. speculated that ABCA1 may be a regulatory protein or may require other protein partners for full activation.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ABCA1 antibody

Immunocytochemistry: ABCA1 Antibody [NB400-105] - ABCA1 antibody was tested at 1:100 in HeLa cells with FITC (green). alpha-Tubulin antibody NB100-690 was tested at a 1:500 dilution using Mouse IgG secondary Antibody NB710-94914 at 1:1000 [HiLyte Fluor 555] (red). Nuclei were counterstained with Dapi (blue). Rabbit Aff - Purified Can, Chk, Hst, Hu, Ms, Por, Rt C, ChIP, E, F, ICC/IF, IP, P, WB
0.1 ml / €330.00
  Novus Biologicals Inc.

ABCA1 antibody

Western Blot: ABCA1 Antibody - Western blot analysis of RAW 264.7 Whole cell Lysate Kit - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2). Rabbit Purified Hst, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCA1 antibody

Western Blot: ABCA1 Antibody - Detection of ABCA1 in wildtype liver tissue (in RIPA) Lane 1: KO mouse liver tissue. Lane 2: WT mouse liver tissue. Mouse HJ1 Purified Hu, Ms, Rt C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCA1 antibody

Western blot analysis of extracts from Jurkat cells, using EMR1 antibody.The lane on the right is treated with the synthesized peptide. Mouse IgG AB.H10 Purified Chk, Hu, Ms E, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABCA1 antibody

Western blot analysis of mouse liver lysate (Lanes 1 and 2: 150ug lysate) and Jurkat cells (Lanes 3 and 4: 105ug) with anti-ABCA1 antibody diluated at 1:2000. (Predicted band size: 254kDa; observed band size: 120~130kDa) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCA1 antibody

Predicted band size: 254 kDa . Positive control: Raw264.7 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCA1 antibody

WB Suggested Anti-Abca1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Mouse Kidney Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ABCA1 (1800-2260) antibody

AM20330PU-N ABCA1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skeletal Muscle at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Mouse IgG1 A00121.01 Aff - Purified Chk, Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ABCA1 (1800-2260) antibody

ABCA1 Mouse IgG1 AB1.G6 Aff - Purified Hu E, IP, P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

ABCA1 antibody

ABCA1 Mouse IgM 1H4 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

ABCA1 antibody

Western blot analysis of Abca1 in wild type liver tissue ( in RIPA ) using Abca1 monoclonal antibody, clone HJ1 ( Cat # MAB2326 ).
Lane 1, KO mouse liver tissue.
Lane 2, WT mouse liver tissue. Mouse IgG2b HJ1 Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ABCA1 antibody

FACS analysis of Raw ( red ) and Raw-cAMP ( blue ) cells using Abca1 monoclonal antibody, clone 5A1-1422.11 ( Cat # MAB2419 ) at a dilution of 1 : 4,000. Rat IgG2a 5A1-1422.11 Ms F
0.1 ml / €470.00
  Abnova Taiwan Corp.

ABCA1 antibody

Western blot analysis of ABCA1 using ABCA1 monoclonal antibody, clone 3A1.891.3 ( Cat # MAB2420 ) at various dilutions in ABCA1 transfected HeLa lysates. Rat IgG1 3A1.891.3 Ms WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ABCA1 antibody

Detection of ABCA1 in mouse peritoneal macrophages using ABCA1 polyclonal antibody ( Cat # PAB12504 ). Lane 1 : T09 uninduced lysate, Lane 2 : T09 induced lysate. Rabbit Hst, Hu, Ms, Rt F, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ABCA1 antibody

ABCA1 Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

ABCA1 antibody

ABCA1 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

ABCA1 antibody

Western blot analysis of ABCA1 expression in A549 (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ABCA1 antibody

Western blot analysis of ABCA1 expression in A549 (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ABCA1 antibody

Western blot analysis of ABCA1 expression in A549 (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ABCA1 antibody

ABCA1 Mouse IgG HJ1 Purified Ms, Rt WB
0.1 mg / €360.00
  Neuromics Antibodies

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