ABCB5 antibody

Principal name

ABCB5 antibody

Alternative names for ABCB5 antibody

ATP-binding cassette sub-family B member 5

SwissProt ID

Q2M3G0 (Human)

Gene ID

340273 (ABCB5)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ABCB5 antibody

Kit Component:
- KN215604G1, ABCB5 gRNA vector 1 in pCas-Guide vector
- KN215604G2, ABCB5 gRNA vector 2 in pCas-Guide vector
- KN215604D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005 May 15;65(10):4320-33.
PMID: 15899824
2. Cheung et al, Gastroenterology. 2011 Jan;140(1):344-55, PMID: 20682318.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ABCB5 antibody

TA303312 (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Immunohistochemistry: ABCB5 Antibody - IHC analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Western blot analysis of Jurkat cells (Lane 1: 45ug lysate), Thp-1 (Lane 2: 60ug lysate) and ECL cells (Lane 3: 60ug) with anti-ABCB5 antibody diluated at 1:2000. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Predicted cell location: Cell membrane, Cytoplasm. Positive control: Human cervical cancer and renal cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue  using ABCB5 antibody at dilution 1/10, on the right is immunohistochemistry of paraffin-embedded Human renal cancer tissue using ABCB5 antibody at dilution 1/10. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Predicted cell location: Cell membrane, Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ABCB5 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

ELISA: ABCB5 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Western blot using affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 µg of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight. Rabbit IgG Aff - Purified Hu, Mky E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

ABCB5 antibody

Western blot using affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 µg of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight. Rabbit IgG Aff - Purified Hu, Mky E, WB
25 µl / €190.00
  Acris Antibodies GmbH

ABCB5 (N-term) antibody

Western blot analysis of anti-ABCB5 Antibody (N-term) in K562 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ABCB5 (N-term) antibody

Human Breast (formalin-fixed, paraffin-embedded) stained with ABCB5 antibody AP22781PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Ig Fraction Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ABCB5 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

ABCB5 antibody

ABCB5 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

ABCB5 antibody

ABCB5 polyclonal antibody ( Cat # PAB7511 ) ( 0.03 µg/ml ) staining of A-431 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ABCB5 antibody

Western blot using ABCB5 polyclonal antibody ( Cat # PAB9925 ) shows detection of ABCB5 beta in ~12.5 µg of transfected-Hi5 whole cell lysate.
No reaction was seen when antibody was pre-incubated with the immunizing peptide ( data not shown ).
A 3-8% Tris-acetate gel was used for separation.
The arrowhead corresponds to 117 kDa ABCB5.
The membrane was probed with the primary antibody at a 1 : 10,000 dilution in 5% milk in TBST at 4°C, overnight.
Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

ABCB5 antibody

Western blot analysis of ABCB5 expression in Hela (A); 293T (B) whole cell lysates. Mouse Aff - Purified Hu P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ABCB5 antibody

Western blot analysis of ABCB5 expression in Hela (A); 293T (B) whole cell lysates. Mouse Aff - Purified Hu P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ABCB5 antibody

Western blot analysis of ABCB5 expression in Hela (A); 293T (B) whole cell lysates. Mouse Aff - Purified Hu P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ABCB5 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, ABCB5 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ABCB5 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, ABCB5 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ABCB5 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, ABCB5 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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