ABCB5 antibody

Principal name

ABCB5 antibody

Alternative names for ABCB5 antibody

ATP-binding cassette sub-family B member 5

SwissProt ID

Q2M3G0 (Human)

Gene ID

340273 (ABCB5)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of ABCB5 antibody

ATP-Binding Cassette proteins comprise a superfamily of transporters with a wide variety of substrates. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. It has been identified as a novel drug transporter in human malignant melanoma. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5.

General readings

1. Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005 May 15;65(10):4320-33.
PMID: 15899824
2. Cheung et al, Gastroenterology. 2011 Jan;140(1):344-55, PMID: 20682318.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ABCB5 antibody

TA303312 (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Immunohistochemistry: ABCB5 Antibody - IHC analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Western blot analysis of Jurkat cells (Lane 1: 45ug lysate), Thp-1 (Lane 2: 60ug lysate) and ECL cells (Lane 3: 60ug) with anti-ABCB5 antibody diluated at 1:2000. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Predicted cell location: Cell membrane, Cytoplasm. Positive control: Human cervical cancer and renal cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue  using ABCB5 antibody at dilution 1/10, on the right is immunohistochemistry of paraffin-embedded Human renal cancer tissue using ABCB5 antibody at dilution 1/10. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Predicted cell location: Cell membrane, Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ABCB5 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

ELISA: ABCB5 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

ABCB5 antibody

Western blot using affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 µg of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight. Rabbit IgG Aff - Purified Hu, Mky E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

ABCB5 antibody

Western blot using affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 µg of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight. Rabbit IgG Aff - Purified Hu, Mky E, WB
25 µl / €190.00
  Acris Antibodies GmbH

ABCB5 (N-term) antibody

Western blot analysis of anti-ABCB5 Antibody (N-term) in K562 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ABCB5 (N-term) antibody

Human Breast (formalin-fixed, paraffin-embedded) stained with ABCB5 antibody AP22781PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Ig Fraction Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ABCB5 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

ABCB5 antibody

ABCB5 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

ABCB5 antibody

ABCB5 polyclonal antibody ( Cat # PAB7511 ) ( 0.03 µg/ml ) staining of A-431 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ABCB5 antibody

Western blot using ABCB5 polyclonal antibody ( Cat # PAB9925 ) shows detection of ABCB5 beta in ~12.5 µg of transfected-Hi5 whole cell lysate.
No reaction was seen when antibody was pre-incubated with the immunizing peptide ( data not shown ).
A 3-8% Tris-acetate gel was used for separation.
The arrowhead corresponds to 117 kDa ABCB5.
The membrane was probed with the primary antibody at a 1 : 10,000 dilution in 5% milk in TBST at 4°C, overnight.
Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

ABCB5 antibody

Western blot analysis of ABCB5 expression in Hela (A); 293T (B) whole cell lysates. Mouse Aff - Purified Hu P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ABCB5 antibody

Western blot analysis of ABCB5 expression in Hela (A); 293T (B) whole cell lysates. Mouse Aff - Purified Hu P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ABCB5 antibody

Western blot analysis of ABCB5 expression in Hela (A); 293T (B) whole cell lysates. Mouse Aff - Purified Hu P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ABCB5 antibody

ELISA: ABCB5 Antibody [44420002] Rabbit Hu E, WB
50 µg / €400.00
  Novus Biologicals Inc.

ABCB5 antibody

Western Blot: ABCB5 Antibody [NB100-93365] - (0.3µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ABCB5 antibody

Western Blot: ABCB5 Antibody [NB110-85481] - detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide. The arrowhead corresponds to 117 kDa ABCB5. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

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