ABCC8 antibody

Principal name

ABCC8 antibody

Alternative names for ABCC8 antibody

HRINS, SUR, SUR1, Sulfonylurea receptor 1, ATP-binding cassette transporter sub-family C member 8

SwissProt ID

Q09428 (Human), Q09429 (Rat)

Gene ID

6833 (ABCC8), 25559 (Abcc8)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Hst (Hamster), Can (Canine), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Dot blot (Dot), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ABCC8 antibody

The relation ship between glucose metabolism and insulin secretion from the pancreatic beta cells is linked with ATP-sensitive potassium channels (KATP). IN response to glucose metabolism, the ATP-sensitive potassium channels are inactivated due to an increase in ATP/ADP ratio, resulting in membrane depolarization and calcium influx through voltage dependent L-type Ca+2 channels. The KATPs are composed 4 units of inwardly rectifying K channel subunit (Kir6.2) and 4 copies of regulatory subunit SUR1 in an octameric complex. SUR 1 is a member of ATP binding cassette super family. SUR receptor confers the sensitivity of Kir6.2 to ATP/ADP sensitivity and to pharmacological agents such as sulfonylurea and diazoxide that close or open the KATP channels. The persistent heyperinsulinimic hypoglycemia in infancy (PHHI) is familial disorder due to defect in negative feed back in response to low glucose levels. SUR1 was mapped on chromosome 11p14-15.1, the same location where the gene for PHHI is located (1). It has been shown that the expression of Kir6.2 and SUR1 are regulated by glucose levels and the actives of glucogon-like peptide receptor1 (2). Abnormal insulin secretion in PHHI appears to be caused by mutations in the SUR gene.

SUR1 is a 1581 amino acid protein with an ATP binding domain with two nucleotide-binding folds (NBFs) and binding sites for sulfonylureas, like glibenclamide, and for channel openers. SUR contains three hydrophobic domains, TM(0), TM(1), and TM(2), with nucleotide binding folds following TM(1) and TM(2). The protein is glycosylated at position 10 on Asn. The SUR1 gene is expressed in at least 4 multiple splice variants ere are multiple splice variants, these splice variants originate from the splicing of the coding region of NFB and lack of axon 17 (SUR1Delta17), axon 19 (SUR1Delta 19) and both (SUR1Delta 17/19) and the fourth is a COOH terminal fragment formed by Exxon 31-39 containing the last 2 TMD and the COOH terminal (SUR1C). The various splice variants of SUR1 are expressed in various tissues with strong expression of SUR1C in cardiomyocytes (3). Only SUR1 and SUR1Delta 17 exhibit high affinity binding sites for sulfonylurea and other pharmacological agents. Several point mutations in the NFB region of SUR1 are also described in NIDDM. A total of five amino acid substitutions and 17 silent mutations were noted by examining all 39 axons of this gene in NIDDM patients form Japan. Two rare novel mutations, D811N in axon 20 and R835C in axon 21, were identified in the first nucleotide-binding fold (NBF), a functionally important region of SUR1, in one patient each, both heterozygotes.

General readings

de Wet H, Rees MG, Shimomura K, Aittoniemi J, Patch AM, Flanagan SE, Ellard S, Hattersley AT, Sansom MS, Ashcroft FM.
Increased ATPase activity produced by mutations at arginine-1380 in nucleotidebinding domain 2 of ABCC8 causes neonatal diabetes.
Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18988-92.
PMID: 18025464

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ABCC8 antibody

  Rabbit Polyclonal SUR1 Antibody  
Immunohistochemistry: SUR1 Antibody - Strong membranous staining pattern observed in colo-rectal cancers by immunohistochemistry.  Image is provided courtesy of the Swedish Human Proteome Resource (HPR) Center. Rabbit IgG Purified Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABCC8 (C-term) antibody

  Goat Polyclonal Antibody against ABCC8  
TA303367 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABCC8 (1548-1582, Cytopl. Dom.) antibody

  Mouse Monoclonal Anti-SUR1 Antibody  
ABCC8 Mouse IgG1 S289-16 Purified Hst, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABCC8 antibody

  Rabbit Polyclonal Anti-ABCC8 Antibody  
WB Suggested Anti-ABCC8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ABCC8 antibody

  Rabbit Polyclonal Anti-ABCC8 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (ABCC8 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABCC8 antibody

Immunoblot versus crude brain membrane preparations from rat (RBM), SUR1 wild-type (WT) and knockout (KO) mice and probed with N289/16 (left), N52A/42 (center) and K89/34 (right) TC supe. Mouse brains courtesy of William Coetzee and Margaret Rice, New York University Medical Center. Mouse IgG1 N289/16 Supernatant Hst, Hu, Ms C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

ABCC8 antibody

Immunoblot versus crude brain membrane preparations from rat (RBM), SUR1 wild-type (WT) and knockout (KO) mice and probed with N289/16 (left), N52A/42 (center) and K89/34 (right) TC supe. Mouse brains courtesy of William Coetzee and Margaret Rice, New York University Medical Center. Mouse IgG1 N289/16 Purified Hst, Hu, Ms C, ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

CRISPR

Catalog No. Species Donor Vector  

ABCC8 - human gene knockout kit via CRISPR

ABCC8 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ABCC8 - human gene knockout kit via CRISPR

ABCC8 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ABCC8 - human gene knockout kit via CRISPR

ABCC8 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ABCC8 - human gene knockout kit via CRISPR

ABCC8 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ABCC8

  Purified recombinant protein of Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8)  
ABCC8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ABCC8 (mGFP-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), 200 uL, >10^7 TU/mL

Lenti ORF particles, ABCC8 (mGFP-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), 200 uL, >10^7 TU/mL  
200 µl / €2,530.00
  OriGene Technologies, Inc.

Lenti ORF particles, ABCC8 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), 200 uL, >10^7 TU/mL

Lenti ORF particles, ABCC8 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), 200 uL, >10^7 TU/mL  
200 µl / €2,280.00
  OriGene Technologies, Inc.

14 Item(s)

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