ABHD12B antibody
Principal name
ABHD12B antibody
Alternative names for ABHD12B antibody
C14orf29, Abhydrolase domain-containing protein 12B
SwissProt ID
Q7Z5M8 (Human)
Gene ID
145447 (ABHD12B)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of ABHD12B antibody
- Kit Component:
- - KN205792G1, ABHD12B gRNA vector 1 in pCas-Guide vector
- KN205792G2, ABHD12B gRNA vector 2 in pCas-Guide vector
- KN205792D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sjoeblom T., et al. (2006) Science. 314:268-274Heilig R., et al. (2003) Nature. 421:601-607
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical analysis of ABHD12B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ABHD12B staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ABHD12B expression in human brain (A); K562 (B) whole cell lysates.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ABHD12B antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP32293PU-N | ABHD12B (C-term) antibody |
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Rabbit | IgG | Aff - Purified | Hu | E, P |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
