ABHD5 antibody

Principal name

ABHD5 antibody

Alternative names for ABHD5 antibody

NCIE2, CGI-58, Abhydrolase domain-containing protein 5

SwissProt ID

Q6QA69 (Rat), Q8WTS1 (Human), Q9DBL9 (Mouse)

Gene ID

51099 (ABHD5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Chk (Chicken), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of ABHD5 antibody

ABHD5 belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in ABHD5 gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

General readings

Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome.Cell Metab. 2006 May;3(5):309-19. PMID: 16679289

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ABHD5 antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1F3 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ABHD5 antibody

ABHD5 polyclonal antibody (A02), Lot # 060103JC01. Western Blot analysis of ABHD5 expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ABHD5 antibody

Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG2a 1F3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ABHD5 antibody

Detection of CGI58 in human skeletal lysate Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABHD5 antibody

TA302449 (1µg/ml) staining of A431 cell line lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABHD5 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ABHD5 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABHD5 antibody

Predicted band size: 39 kDa . Positive control: A431 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABHD5 antibody

WB Suggested Anti-ABHD5 Antibody Titration: 5.0 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABHD5 antibody

WB Suggested Anti-ABHD5 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate; ABHD5 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ABHD5 antibody

ABHD5 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ABHD5 antibody

ABHD5 monoclonal antibody (M02), clone 4B12 Western Blot analysis of ABHD5 expression in A-431 ( Cat # L015V1 ). Mouse IgG1 4B12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ABHD5 antibody

ABHD5 polyclonal antibody ( Cat # PAB7219 ) ( 1 µg/ml ) staining of A-431 cell line lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Bov, Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ABHD5 antibody

Western blot analysis of ABHD5 expression in NIH/3T3 cell lysate. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ABHD5 antibody

Western blot analysis of ABHD5 in ABHD5 overexpressed lysates using ABHD5 polyclonal antibody ( Cat # PAB12500 ). Rabbit Hu WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ABHD5 antibody

Transfected 293T; WB Suggested Anti-ABHD5 Antibody Titration: 5.0ug/ml. Positive Control: Transfected 293T; ABHD5 antibody - N-terminal region (ARP42455_T100) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

ABHD5 antibody

Human Colon; ABHD5 antibody - C-terminal region (ARP42456_P050) in Human Colon cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

ABHD5 antibody

Western Blot: ABHD5 Antibody [NB100-57850] - NB100-57850 (1µg/ml) staining of A431 cell line lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ABHD5 antibody

ABHD5 Rabbit Biotin Hu WB
0.1 ml / €360.00
  Novus Biologicals Inc.

ABHD5 antibody

ABHD5 Rabbit DyLight488 Hu WB
0.1 ml / €390.00
  Novus Biologicals Inc.

ABHD5 antibody

ABHD5 Rabbit HRP Hu WB
0.1 ml / €390.00
  Novus Biologicals Inc.

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