ABL1 antibody

Principal name

ABL1 antibody

Alternative names for ABL1 antibody

c-ABL, p150, JTK7, bcr/abl, Proto-oncogene tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1

SwissProt ID

P00519 (Human), P00520 (Mouse), Q61258 (Mouse)

Gene ID

25 (ABL1), 11350 (Abl1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Mky (Monkey), Rt (Rat), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Enzyme Immunoassay (E), Frozen Sections (C)

Background of ABL1 antibody

Protein tyrosine kinases play important roles in the transduction of extracellular signals. Receptor tyrosine kinases include a myriad of growth factor receptors, which are activated upon ligand binding. Differential binding of adapter proteins may impart some signal specificity, since many of the receptors use the same adapter proteins, and may also link the activation of the receptor to multiple pathways. In contrast, nonreceptor tyrosine kinases are recruited to substrates and or activators by their SH2 and/or SH3 domains. These domains also allow them to interact with activated receptor tyrosine kinases. These kinases are divided into several groups, including the Src, Jak, Abl, Fak, Fps, Csk, Syk and Btk families, and are components of intracellular signaling cascades. There is a lot of interest in these kinases because many of them regulate different pathways and have been identified as oncogenes or components of oncogenic pathways. The Abl oncogen is implicated in several human leukemias including the majority of chronic myelocytic leukemia (CML), one fourth of adult acute lymphoblastic leukemia (ALL) and few of pediatric ALL. In these leukemias the c-abl proto-oncogene undergoes a chromosomal translocation (9;22) producing the Philadelphia (Ph1) chromosome. Molecular analysis of the breakpoints in this translocated region reveals the involvement of the c-abl locus from chromosome 9 and small DNA segment on chromosome 22, designated as the breakpoint cluster region ( bcr), resulting in the formation of a fused bcr/abl gene. The resulting chimeric sequence encodes for an abnormal hybrid protein (120 kD) that possesses a tyrosine kinase activity and an SH2 domain, not present in the normal c-abl encoded protein, but similar to that of the protein product of the transforming viral abl gene. A majority of the known protein kinases are localized at or near the plasma membranes. The c-Abl tyrosine kinase is unusual in that it has both nuclear and cytoplasmic functions. Several cytoplasmic substrates of c-Abl have been identified. These include the SH2/SH3 adaptor protein Crk and the Crk-binding protein p130cas. Nuclear c-Abl has been implicated in the regulation of cell cycledependent and DNA damage-induced gene expression. The c-Abl protein contains three high mobility group-like domains that bind to A+T-rich DNA in a cooperative manner. While c-Abl does not select DNA sequences, it can be recruited to specific DNA-binding complexes through protein-protein interactions. Thus for instance, c-Abl interacts with the transcription factor RXF1, which binds to the palindromic EP sequence in the hepatitis virus B enhancer, and can also be recruited to an E2FDNA binding complex, through a direct interaction with the retinoblastoma protein (RB). The p53-related protein p73, is also a substrate of the non-receptor tyrosine kinase c-Abl, in response to DNA damage. Apoptotic activity of p73a requires the presence of functional, kinase-competent c-Abl. Furthermore, p73 and c-Abl can associate with each other, and this binding is mediated by PxxP motif in p73 and the SH3 domain of c-Abl. The ability of c-Abl to phosphorylate p73 is markedly increased by g-irradiation. Antibodies reacting specifically with c-Abl are useful tools in the study of the detailed mechanisms of the signaling pathways involving c-Abl.

General readings

General / background references:Whang YE et al. c-Abl is required for development and optimal cell proliferation in the context of p53 deficiency. Proc Natl Acad Sci U S A 97:5486-91 (2000). PubMed PMID: 10805805
Sun X et al. Activation of the cytoplasmic c-Abl tyrosine kinase by reactive oxygen species. J Biol Chem 275:17237-40 (2000). PubMed PMID: 10770918

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ABL1 antibody

Human placenta stained with Anti-c-Abl antibody Rabbit Purified Hu, Ms P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

ABL1 antibody

Human placenta stained with Anti-c-Abl antibody Rabbit Purified Hu, Ms P, WB
1 ml / €440.00
  Acris Antibodies GmbH

ABL1 antibody

Human placenta stained with Anti-c-Abl antibody Rabbit Purified Hu, Ms P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

ABL1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Abl antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Western blot analysis of extracts from COS7 cells, treated with Adriamycin (0.5?g/ml, 24hours), using Abl (Ab-204) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using c-Abl antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Western blot analysis of extracts from 293 cells, using ABL1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Western blot analysis of Abl expression in Adriamycin treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Western blot analysis of c-Abl expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Western blot analysis of c-Abl expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Western blot analysis of extracts from HL60 cells using phospho-ABL1/2 -Y393/429 antibody and the same antibody preincubated with blocking peptide. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Western Blot: c Abl Antibody [TA336552] - Detection of C-ABL in K562 whole cell lysate using TA336552. Rabbit Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ABL1 antibody

Western blot analysis using ABL1 antibody Cat.-No AM06256SU-N against truncated GST-ABL1 recombinant protein (1). Mouse IgG1 7B11D6 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

ABL1 pTyr134 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ABL1 pTyr245 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ABL1 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ABL1 (N-term) antibody

Western blot analysis of ABL1 Antibody (N-term) in Y79 cell line lysates (35ug/lane). ABL1(arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ABL1 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ABL1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ABL1 antibody

Western blot analysis of Abl  antibody (Cat.-No.: AP20341PU-N) in extracts from COS7 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ABL1 antibody

Immunohistochemistry analysis of c-Abl antibody Cat.-No. AP20540PU-N in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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