ACAP2 / CENTB2 antibody

Principal name

ACAP2 / CENTB2 antibody

Alternative names for ACAP2 / CENTB2 antibody

KIAA0041, Centaurin beta 2, ARFGAP with coiled-coil, ANK repeat and PH domain-containing protein 2

SwissProt ID

Q15057 (Human), Q5FVC7 (Rat), Q6ZQK5 (Mouse)

Gene ID

23527 (ACAP2)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of ACAP2 / CENTB2 antibody

Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.

General readings

Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA.
ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.
J Cell Biol. 2000 Oct 30;151(3):627-38.
PMID: 11062263

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACAP2 / CENTB2 (C-term) antibody

TA302897 (1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACAP2 / CENTB2 (766-778) antibody

Lung: Formalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hu, Mky, Ms, Platypus, Prim, Rb, Rt, Tr E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ACAP2 / CENTB2 antibody

ACAP2 / CENTB2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ACAP2 / CENTB2 antibody

Western Blot analysis of ACAP2 expression in transfected 293T cell line (H00023527-T01) by ACAP2 MaxPab polyclonal antibody.

Lane 1: CENTB2 transfected lysate(85.58 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ACAP2 / CENTB2 antibody

Western Blot analysis of ACAP2 expression in transfected 293T cell line (H00023527-T02) by ACAP2 MaxPab polyclonal antibody.

Lane 1: CENTB2 transfected lysate(88.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ACAP2 / CENTB2 antibody

CENTB2 monoclonal antibody (M01), clone 4G3 Western Blot analysis of CENTB2 expression in HeLa ( Cat # L013V1 ). Mouse IgG1 4G3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ACAP2 / CENTB2 antibody

ACAP2 polyclonal antibody ( Cat # PAB6494 ) staining ( 1 µg/ml ) of MOLT4 lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ACAP2 / CENTB2 antibody

NB100-1321 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ACAP2 / CENTB2 antibody

Immunohistochemistry-Paraffin: Centaurin beta 2 Antibody [NBP1-84543] - Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

ACAP2 / CENTB2 antibody

Immunohistochemistry-Paraffin: Centaurin beta 2 Antibody [NBP1-84544] Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ACAP2 / CENTB2

ACAP2 / CENTB2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ACAP2 / CENTB2

ACAP2 / CENTB2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ACAP2 / CENTB2

ACAP2 / CENTB2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ACAP2 / CENTB2

ACAP2 / CENTB2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ACAP2 / CENTB2

ACAP2 / CENTB2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

ACAP2 (Human) IP-WB Antibody Pair

ACAP2 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CENTB2 293T Cell Transient Overexpression Lysate(Denatured)

CENTB2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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