ACAT1 / MAT antibody

Principal name

ACAT1 / MAT antibody

Alternative names for ACAT1 / MAT antibody

Acetyl-CoA acetyltransferase mitochondrial, Acetoacetyl-CoA thiolase T2

SwissProt ID

P17764 (Rat), P24752 (Human), Q8QZT1 (Mouse)

Gene ID

38 (ACAT1), 110446 (Acat1), 25014

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Por (Porcine), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ACAT1 / MAT antibody

ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The gene encoding ACAT1 spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by uriry excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. This gene spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by uriry excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. American journal of physiology. Endocrinology and metabolism 2009 Aug 297 (2): E474-82.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACAT1 / MAT antibody

  Mouse anti-ACAT1 monoclonal antibody  
Western blot analysis of extracts from COLO205 cells, using EDG4 antibody.The lane on the right is treated with the synthesized peptide. Mouse IgG AT1.H11 Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACAT1 / MAT antibody

  Rabbit anti-ACAT1 polyclonal antibody  
Western blot analysis of rat liver lysate (Lane 1: 65ug lysate), Jurkat cells (Lane 2: 28ug lysate) and SGC cells (Lane 3: 33ug lysate) with anti-ACAT1 antibody diluated at 1:2000. (Predicted and observed size: 65kDa) Rabbit IgG Purified Hu, Por, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACAT1 / MAT (253-266) antibody

  Goat Anti-ACAT1 (aa253-266) Antibody  
(0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACAT1 / MAT antibody

  Rabbit anti-ACAT1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ACAT1 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACAT1 / MAT antibody

  Rabbit Polyclonal Anti-ACAT1 Antibody  
WB Suggested Anti-ACAT1 Antibody Titration: 1 ug/ml; Positive Control: HepG2 cell lysate. ACAT1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ACAT1 / MAT antibody

  Rabbit Polyclonal Anti-ACAT1 Antibody  
WB Suggested Anti-ACAT1 Antibody Titration: 1 ug/ml; Positive Control: Fetal heart lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ACAT1 / MAT (257-269) antibody

  Goat Polyclonal Anti-ACAT1 (aa257-269) Antibody  
TA348998 (0.01 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACAT1 / MAT (N-term) antibody

Human Adrenal Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE). Mouse IgG AT1.H11 Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ACAT1 / MAT antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT15E5 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ACAT1 / MAT antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT15E5 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

ACAT1 / MAT (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ACAT1 / MAT antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

ACAT1 / MAT antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Por, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

ACAT1 / MAT antibody

Human Liver, Hephatocytes: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

ACAT1 / MAT antibody

Western blot analysis of extracts from HepG2/Jurkat/293/A549 cells, using ACAT1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

ACAT1 / MAT antibody

Western blot analysis of extracts from HepG2/Jurkat/293/A549 cells, using ACAT1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

ACAT1 - human gene knockout kit via CRISPR

ACAT1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ACAT1 - human gene knockout kit via CRISPR

ACAT1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ACAT1 - human gene knockout kit via CRISPR

ACAT1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ACAT1 - human gene knockout kit via CRISPR

ACAT1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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