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Acetyl-CoA carboxylase 1 antibody

Principal name

Acetyl-CoA carboxylase 1 antibody

Alternative names for Acetyl-CoA carboxylase 1 antibody

ACACA, ACAC, ACC1, ACCA

SwissProt ID

P11497 (Rat), Q13085 (Human), Q5SWU9 (Mouse)

Gene ID

31 (ACACA), 107476 (Acaca)

Ncbi ID

9606, NP_942131.1, NP_579938.2, NM_198834

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of Acetyl-CoA carboxylase 1 antibody

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle. ACC-alpha (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACC-beta (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACC-alpha is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACC-alpha, which maps to human chromosome 17, encodes the 265 kDa alpha form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACC-beta is homologous to that of ACC-alpha except for an additional peptide of about 150 amino acids at the N-terminus.

General readings

Ray H. et al. Cell cycle regulation of the BRCA1/acetyl-CoA-carboxylase complex.Biochemical and Biophysical Research Communications. Volume 378, Issue 3, 16 January 2009, Pages 615-619

H00000031-M01 - Acetyl-CoA carboxylase 1

Detection limit for recombinant GST tagged ACACA is approximately 0.03ng/ml as a capture antibody.

H00000031-M01

Immunohistochemistry (IHC) analyzes of ACC1 antibody (Cat.-No.: AP06366PU-N) in paraffin-embedded human skeletal muscle brain tissue.

AP06366PU-N

Western blot (WB) analysis of ACCα antibody (Cat.-No.: AP06366PU-N) antibody at 1/500 dilution
Lane1:Hela whole cell lysate
Lane2:Jurkat whole cell lysate
Lane3:NIH-3T3 whole cell lysate
Lane4:PC12 whole cell lysate

AP06366PU-N

Immunohistochemistry (IHC) analyzes of ACCA antibody (Cat.-No.: AP06367PU-N) in paraffin-embedded human breast carcinoma colon carcinoma tissue.

AP06367PU-N

Western blot (WB) analysis of ACCα antibody (Cat.-No.: AP06367PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate
Lane2:NIH-3T3 whole cell lysate

AP06367PU-N

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 5', using Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACC1 (Phospho-Ser80) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from K562 cells treated with Insulin 0.01U/ml 15', using ACC1 (Phospho-Ser80) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Acetyl-CoA Carboxylase (Ab-80) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells, treated with PMA 125ng/ml 15', using Acetyl-CoA Carboxylase (Ab-80) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using ACC1 (Ab-80) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells, treated with PMA 125ng/ml 30', using ACC1 (Ab-80) Antibody. The lane on the right is treated with the synthesized peptide.

CPA3108-100ul - Acetyl-CoA carboxylase 1

Immunohistochemical analysis of ACC alpha staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of ACC alpha expression in HepG2 (A); NIH3T3 (B) whole cell lysates.

Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (125ng/ml, 30mins), using ACC1 (Ab-80) antibody.The lane on the right is treated with the synthesized peptide.

TA313410

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ACC1 (Ab-80) antibody.The picture on the right is treated with the synthesized peptide.

TA313410

Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 15min), using Acetyl-CoA Carboxylase (Ab-80) antibody.The lane on the right is treated with the synthesized peptide.

TA313414

Western blot analysis of extracts from HuvEc cells (Lane 2) and 3T3 cells (Lane 3), using Acetyl-CoA Carboxylase (Ab-80) Antibody. The lane on the left is treated with synthesized peptide.

TA313414

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Acetyl-CoA Carboxylase (Ab-80) antibody.The picture on the right is treated with the synthesized peptide.

TA313414

Predicted band size: 266 kDa . Positive control: Heia and HUVEC cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: Heia and HUVEC cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321969

Western blot analysis of ACC1 expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325203

Predicted band size: 266 kDa . Positive control: 293 cells treated with AICAR lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with AICAR lysate Lane2: 293 cells treated with AICAR lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321968

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA313414	Acetyl-CoA carboxylase 1 antibody
	Rabbit polyclonal Acetyl-CoA Carboxylase (Ab-80) antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+2 additional images
TA321968	Acetyl-CoA carboxylase 1 antibody
	Anti-ACACA (Phospho-Ser79) Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA321969	Acetyl-CoA carboxylase 1 antibody
	Anti-ACACA Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA325203	Acetyl-CoA carboxylase 1 antibody
	Rabbit Polyclonal ACC1 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
AP06366PU-N	Acetyl-CoA carboxylase 1 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH
+1 additional image
AP06367PU-N	Acetyl-CoA carboxylase 1 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH
+1 additional image
AP54757SU-N	Acetyl-CoA carboxylase 1 pSer1263 antibody
	Rabbit		Purified	Hu	E, ICC/IF, WB	0.1 mg / €430.00
						OriGene Technologies GmbH

Human Lenti ORF Particles

Catalog No.
RC218293L1V	Lenti ORF particles, ACACA (Myc-DDK tagged) - Human acetyl-CoA carboxylase alpha (ACACA), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,420.00
		OriGene Technologies, Inc.

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