Acetylcholine receptor alpha subunit antibody

Principal name

Acetylcholine receptor alpha subunit antibody

Alternative names for Acetylcholine receptor alpha subunit antibody

CHRNA1, ACHRA, CHNRA

SwissProt ID

P02708 (Human), P02709 (Bovin), P04756 (Mouse), P25108 (Rat)

Gene ID

1134 (CHRNA1), 79557 (Chrna1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Amph (Amphibian), Fi (Fish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E)

Background of Acetylcholine receptor alpha subunit antibody

Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (ChRs) which belong to the ligand-gated ion channel superfamily. ChRs also respond to nicotine, hence their me.To date, 17 different but related subunits of ChRs have been identified and cloned. They consist of a subunits (a1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding. The other subunits responsible for making up the active receptor are the Ã? (Ã?1-4), ?, d and e subunits. Structurally, all subunits have the following: a conserved large extracellular N-termil domain, conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-termil domain. An active ChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore.All a subunits are expressed in neurol cells except for the a1 subunit which is specifically expressed in skeletal muscle. Indeed, two different ChR structural entities with the following stoichiometry are observed in this tissue: (a1)2Ã?1d? in fetal muscle cells and (a1)2Ã?1de at mature neuromuscular sypses.The a1 extracellular domain contains the main immunogenic region, a region against which a large fraction of autoantibodies against ChR is directed in the autoimmune disease myasthenia gravis (MG). This autoimmune disease is characterized by the malfunction of neuromuscular transmission as a result of nonfunctiol ChRs, leading to defective sigling at the neuromuscular junction.Due to its central role in muscle contraction and autonomic nervous system, ChRs have evolved to be important targets of toxins secreted by plants, insects and animals. One prominent toxin affecting a1, in addition to other subunits, is a-bungarotoxin, a ske toxin which blocks ChRs.

General readings

This product has been used in:Froehner SC et al. Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor. J Biol Chem 258:7112-20 (1983). PubMed PMID: 6189834
General / background references:Xie MH et al. Direct demonstration of MuSK involvement in acetylcholine receptor clustering through identification of agonist ScFv. Nat Biotechnol 15:768-71 (1997). PubMed PMID: 9255792
Bloch RJ et al. Association of acetylcholine receptors with peripheral membrane proteins: evidence from antibody-induced coaggregation. J Membr Biol 138:13-28 (1994). PubMed PMID: 8189428
Karlin A Structure of nicotinic acetylcholine receptors. Curr Opin Neurobiol 3:299-309 (1993). PubMed PMID: 8369624

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Acetylcholine receptor alpha subunit antibody

Western blot analysis of rat skeletal muscle (lanes 1 and 3) and mouse C2C12 myoblasts (lanes 2 and 4): 1, 2. Anti-Nicotinic Acetylcholine Receptor a1(extracellular) antibody , (1:200). 3, 4. Anti-Nicotinic Acetylcholine Receptor a1(extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

WB Suggested Anti-CHRNA1 Antibody Titration: 0.12ug/ml; Positive Control: Human heart Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

Western blot analysis of extracts of various cell lines, using CHRNA1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

WB Suggested Anti-CHRNA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

WB Suggested Anti-CHRNA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:2500; Positive Control: Human kidney Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

Immunohistochemistry on Paraffin Sections of Rat brain using AP23320PU-N CHRNA1 antibody. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Rabbit Serum Hu, Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Rabbit Purified Hu, Ms C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Mouse IgG1 G10 Ascites Hu, Ms C, F, IP
0.5 ml / €440.00
  Acris Antibodies GmbH

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Mouse IgG2a 2G5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Acetylcholine receptor alpha subunit antibody

Immunohistochemical staining of CHRNA1 on formalin fixed, paraffin embedded rat brain with CHRNA1 polyclonal antibody ( Cat # PAB12331 ). Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Acetylcholine receptor alpha subunit antibody

Human Jurkat; WB Suggested Anti-CHRNA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; CHRNA1 antibody - N-terminal region (ARP34909_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

Acetylcholine receptor alpha subunit antibody

Human kidney; WB Suggested Anti-CHRNA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: Human kidney; CHRNA1 antibody - N-terminal region (ARP34910_P050) in Human kidney cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Acetylcholine receptor alpha subunit antibody

Human Intestine; CHRNA1 antibody - C-terminal region (AVARP13084_P050) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Acetylcholine receptor alpha subunit antibody

Immunohistochemical analysis of CHRNA1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Acetylcholine receptor alpha subunit antibody

Immunohistochemical analysis of CHRNA1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Acetylcholine receptor alpha subunit antibody

Immunohistochemical analysis of CHRNA1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Mouse IgG1 G10 Ascites Hu, Ms C, F, IP, P
0.25 ml / €360.00
  Novus Biologicals Inc.

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Mouse IgG1 88B Ascites Amph, Chk, Fi, Ms, Rt C, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

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