Acetylcholine receptor alpha subunit antibody

Principal name

Acetylcholine receptor alpha subunit antibody

Alternative names for Acetylcholine receptor alpha subunit antibody

CHRNA1, ACHRA, CHNRA

SwissProt ID

P02708 (Human), P02709 (Bovin), P04756 (Mouse), P25108 (Rat)

Gene ID

1134 (CHRNA1), 79557 (Chrna1), 11435

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Torpedo

Available hosts

Rabbit, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Acetylcholine receptor alpha subunit antibody

Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (ChRs) which belong to the ligand-gated ion channel superfamily. ChRs also respond to nicotine, hence their me.To date, 17 different but related subunits of ChRs have been identified and cloned. They consist of a subunits (a1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding. The other subunits responsible for making up the active receptor are the Ã? (Ã?1-4), ?, d and e subunits. Structurally, all subunits have the following: a conserved large extracellular N-termil domain, conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-termil domain. An active ChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore.All a subunits are expressed in neurol cells except for the a1 subunit which is specifically expressed in skeletal muscle. Indeed, two different ChR structural entities with the following stoichiometry are observed in this tissue: (a1)2Ã?1d? in fetal muscle cells and (a1)2Ã?1de at mature neuromuscular sypses.The a1 extracellular domain contains the main immunogenic region, a region against which a large fraction of autoantibodies against ChR is directed in the autoimmune disease myasthenia gravis (MG). This autoimmune disease is characterized by the malfunction of neuromuscular transmission as a result of nonfunctiol ChRs, leading to defective sigling at the neuromuscular junction.Due to its central role in muscle contraction and autonomic nervous system, ChRs have evolved to be important targets of toxins secreted by plants, insects and animals. One prominent toxin affecting a1, in addition to other subunits, is a-bungarotoxin, a ske toxin which blocks ChRs.

General readings

Webster,R., et al., (2004) Neurology 62 (7), 1090-1096

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Acetylcholine receptor alpha subunit antibody

  Rabbit Polyclonal Anti-Nicotinic Acetylcholine Receptor alpha1 (extracellular)  
Western blot analysis of rat skeletal muscle (lanes 1 and 3) and mouse C2C12 myoblasts (lanes 2 and 4): 1, 2. Anti-Nicotinic Acetylcholine Receptor a1(extracellular) antibody , (1:200). 3, 4. Anti-Nicotinic Acetylcholine Receptor a1(extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

  Rabbit Polyclonal Anti-CHRNA1 Antibody  
WB Suggested Anti-CHRNA1 Antibody Titration: 0.12ug/ml; Positive Control: Human heart Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

  Rabbit anti-CHRNA1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CHRNA1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

  Rabbit Polyclonal Anti-CHRNA1 Antibody  
WB Suggested Anti-CHRNA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

  Rabbit Polyclonal Anti-CHRNA1 Antibody  
WB Suggested Anti-CHRNA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:2500; Positive Control: Human kidney Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

  Rabbit Polyclonal Anti-CHRNA1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 60 ug, Lane: Mouse heart tissue, Primary antibody: (CHRNA1 Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

  Rat Monoclonal anti-nAchR, a1, a3 & a5 Subunits Antibody  
Clustered acetylcholine receptors in transfected QT6 cells visualized by immunofluorescence using MAb 210. Photo courtesy of M. Maimone, SUNY Syracuse. Rat IgG1 210 Purified Can, Chk, Hu, Ms, Rt E, F, ICC/IF, IP, WB
500 µl / €379.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

  Rat Monoclonal anti-nAchR, a1-Subunit Antibody  
Acetylcholine receptor alpha subunit Rat IgG2a 61 Purified Hu, Ms, Torpedo ICC/IF, IP
500 µl / €379.00
  OriGene Technologies, Inc.

Acetylcholine receptor alpha subunit antibody

Immunohistochemistry on Paraffin Sections of Rat brain using AP23320PU-N CHRNA1 antibody. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Rabbit Serum Hu, Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Acetylcholine receptor alpha subunit antibody

Acetylcholine receptor alpha subunit Rabbit Purified Hu, Ms C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CHRNA1 - human gene knockout kit via CRISPR

CHRNA1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CHRNA1 - human gene knockout kit via CRISPR

CHRNA1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CHRNA1 - human gene knockout kit via CRISPR

CHRNA1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CHRNA1 - human gene knockout kit via CRISPR

CHRNA1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Chrna1 - mouse gene knockout kit via CRISPR

Chrna1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Chrna1 - mouse gene knockout kit via CRISPR

Chrna1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Chrna1 - mouse gene knockout kit via CRISPR

Chrna1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Chrna1 - mouse gene knockout kit via CRISPR

Chrna1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CHRNA1 (mGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, CHRNA1 (mGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,100.00
  OriGene Technologies, Inc.

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