Acetylcholinesterase antibody

Principal name

Acetylcholinesterase antibody

Alternative names for Acetylcholinesterase antibody

AChE, Acetylcholine Esterase, Acetylcholine-Esterase

SwissProt ID

O42275 (Eleel), O62763 (Felca), P21836 (Mouse), P22303 (Human), P23795 (Bovin), P37136 (Rat)

Gene ID

540446 (ACHE), 43, 11423 (Ache), 83817

Available reactivities

Bov (Bovine), Hu (Human), Fi (Fish), Rt (Rat), Ms (Mouse), Rb (Rabbit), Can (Canine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Goat, Sheep, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R)

Background of Acetylcholinesterase antibody

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

General readings

Cottingham MG, Voskuil JL, Vaux DJ. The intact human acetylcholinesterase C-terminal oligomerization domain is alphahelical in situ and in isolation, but a shorter fragment forms beta-sheet-rich amyloid fibrils and protofibrillar oligomers. Biochemistry. 2003 Sep 16;42(36):10863-73.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Acetylcholinesterase antibody

  Rabbit polyclonal antibody to AChE (acetylcholinesterase (Yt blood group))  
Sample (30 ug of whole cell lysate). A: Raji. 7.5% SDS PAGE. TA308776 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase (C-term) antibody

  Goat Polyclonal Antibody against ACHE  
TA302997 (0.3µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide.  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase antibody

  Rabbit polyclonal antibody to AChE (acetylcholinesterase (Yt blood group))  
Sample (30 ug of whole cell lysate). A: Hep G2 . B: Molt-4 . 7.5% SDS PAGE. TA308775 diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase (N-term) antibody

  Rabbit polyclonal ACHE Antibody (N-term)  
ACHE Antibody (N-term) (Cat. #TA324994) western blot analysis in Jurkat,Raji,Y79 cell line lysates (35ug/lane).This demonstrates the ACHE antibody detected the ACHE protein (arrow). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase antibody

  Rabbit polyclonal ACHE antibody  
Western blot analysis of extracts from HepG2 cells, using ACHE antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase antibody

  Mouse monoclonal anti-ACHE antibody (C-term)  
ACHE Antibody (C-term) (Cat. #TA328141) western blot analysis in Raji,Jurkat,COS7,mouse NIH/3T3 cell line and mouse cerebellum,rat cerebellum tissue lysates (35ug/lane).This demonstrates the ACHE antibody detected the ACHE protein (arrow). Mouse IgG1 684CT8.3.4 Purified Bov, Hu, Ms, Rb, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase antibody

  Rabbit Polyclonal Anti-ACHE Antibody - middle region  
WB Suggested Anti-ACHE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase antibody

  Rabbit Polyclonal Anti-ACHE Antibody - N-terminal region  
WB Suggested Anti-ACHE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt, Ze ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase antibody

  Rabbit Polyclonal Anti-ACHE Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: (ACHE Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Acetylcholinesterase antibody

Acetylcholinesterase Rabbit IgG Aff - Purified Electric eel, Fi, Hu E, WB
1 ml / €330.00
  Acris Antibodies GmbH

Acetylcholinesterase (N-term) antibody

Skeletal muscle: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Western blot (WB) analysis of AChE Antibody Cat.-No AP20787PU-N in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Acetylcholinesterase Rabbit IgG Azide Free Electric eel Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €240.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Acetylcholinesterase Rabbit IgG Biotin Electric eel Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €350.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Acetylcholinesterase Rabbit IgG Aff - Purified Electric eel Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €440.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Acetylcholinesterase Mouse IgG1 AE-2 Ascites Hu C, IP
0.1 ml / €410.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Acetylcholinesterase Sheep Purified Hu E, P
1 ml / €330.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Acetylcholinesterase Goat Serum Bov E, IP, WB
2 ml / €1,180.00
  Acris Antibodies GmbH

Acetylcholinesterase antibody

Acetylcholinesterase Goat Biotin Bov E, IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

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