Acid ceramidase antibody

Principal name

Acid ceramidase antibody

Alternative names for Acid ceramidase antibody

ASAH1, ASAH, Acylsphingosine deacylase, PHP32, N-acylsphingosine amidohydrolase, Putative 32 kDa heart protein

SwissProt ID

Q13510 (Human), Q9WV54 (Mouse)

Gene ID

427 (ASAH1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of Acid ceramidase antibody

Acid ceramidase catalyzes the degradation of ceramide in normal tissues, and deficiency leads to accumulation of ceramide in tissues, a hallmark of Farber disease. Effected individuals experience early onset joint problems and neurological problems, owing to mutations in the acid ceramidase gene. Bioinformatic analysis of gene expression also reveals acid ceramidase to be among the 5 most important genes associated with melanoma. In addition to ceramide hydrolysis, purified acid ceramidase also exhibits the ability to catalyze ceramide synthesis, utilizing [14C]lauric acid and sphingosine as substrates. Interestingly, pH regulates which reaction is favored; for hydrolysis the pH optimum is 4.5, whereas for the reverse reaction favors a pH of 5.5, further supporting a complex and central role for acid ceramidase in sphingolipid metabolism.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Acid ceramidase (25-125) antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG3 1A7 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Acid ceramidase (C-term) antibody

Western blot analysis of ASAH1 in mouse heart tissue lysate with ASAH1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Acid ceramidase antibody

WB Suggested Anti-ASAH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acid ceramidase antibody

WB Suggested Anti-ASAH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acid ceramidase antibody

Western Blot analysis of ASAH1 expression in transfected 293T cell line (H00000427-T02) by ASAH1 MaxPab polyclonal antibody.

Lane 1: ASAH1 transfected lysate(44.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Acid ceramidase antibody

Immunoperoxidase of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml] Mouse IgG3 2C9 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Acid ceramidase antibody

Immunoperoxidase of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG3 1A7 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Acid ceramidase antibody

Western blot analysis of ASAH1 in mouse heart tissue lysate with ASAH1 polyclonal antibody ( Cat # PAB13056 ) at ( A ) 1 and ( B ) 2 µg/ml. Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Acid ceramidase antibody

Human Lung; WB Suggested Anti-ASAH1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Lung; ASAH1 antibody - N-terminal region (ARP57760_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Acid ceramidase antibody

Human THP-1; WB Suggested Anti-ASAH1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; ASAH1 antibody - middle region (ARP57761_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Acid ceramidase antibody

Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-76933] - Human heart tissue with ASAH1 antibody at 2.5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Acid ceramidase antibody

Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Immunohistochemical staining of human epididymis shows strong granular positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Acid ceramidase (transcript variant 2)

Acid ceramidase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Acid ceramidase

Acid ceramidase Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

Acid ceramidase

Acid ceramidase Human
10 µg / €380.00
  Abnova Taiwan Corp.

Acid ceramidase

Acid ceramidase Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Acid ceramidase

Acid ceramidase Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

Acid ceramidase Lysate (Non-Denatured)

Acid ceramidase Lysate (Non-Denatured)  
0.1 mg / €270.00
  Abnova Taiwan Corp.

18 Item(s)

per page
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