ACO2 antibody

Principal name

ACO2 antibody

Alternative names for ACO2 antibody

Aconitase 2, Aconitate hydratase mitochondrial

SwissProt ID

Q99798 (Human)

Gene ID

50 (ACO2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Ce (C. elegans), Por (Porcine), Can (Canine), Chk (Chicken), Dros (Drosophila), African clawed frog, Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)

Background of ACO2 antibody

ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Bota DA, Davies KJ. Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism. Nat Cell Biol. 2002 Sep;4(9):674-80.
PMID: 12198491

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACO2 antibody

Western blot analysis of anti-ACO2 (AP11745PU-N) in mouse heart (left) and 293 (right) tissue lysates (35 µg/lane). ACO2 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu, Ms, Rt P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ACO2 (541-555) antibody

TA349000 (0.01 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500824, Dilution 1:50) Mouse IgG1 OTI3G8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ACO2 antibody

Anti-ACO2 mouse monoclonal antibody (TA500841) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307). Mouse IgG1 OTI7A11 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ACO2 antibody

Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500873). Mouse IgG1 OTI7G4 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ACO2 (C-term) antibody

Human Pineal Tissue; Rabbit Anti-ACO2 Antibody . Catalog Number: ARP56302_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in cell bodies of penealocytes and connective tissue (septum . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-ACO2 Antibody (ARP56302_P050) ) in Human Pineal Tissue using Immunohistochemistry Rabbit Aff - Purified Bov, Ce, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ACO2 antibody

Western blot analysis of ACO2 (arrow) using rabbit polyclonal ACO2 Antibody (Center) (Cat.#TA302090).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACO2 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

(0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

Predicted band size: 85 kDa. Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

Predicted band size: 85 kDa. Positive control: Mouse heart tissue and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Mouse heart tissue lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

Anti-ACO2 mouse monoclonal antibody (TA500913) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307). Mouse IgG2a OTI3G10 Purified Hu F, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ACO2 antibody

Human Heart (formalin-fixed, paraffin-embedded) stained with ACO2 antibody AP22773PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ACO2 antibody

Western Blot analysis of ACO2 expression in transfected 293T cell line (H00000050-T02) by ACO2 MaxPab polyclonal antibody.

Lane 1: ACO2 transfected lysate(85.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ACO2 antibody

ACO2 Mouse IgG2a 1A11 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ACO2 antibody

Perfused isolated rat heart whole tissue lysate was lysed with either A ) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4 , 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B ) T-PER Tissue Protein Extraction Reagent ( Pierce ) , containing 1mM Na3VO4 , 20 mM NaF, 5 v/v % protease inhibitor cocktail ( Sigma ) ; PVDF membrane was incubated in primary Ab ACO2 polyclonal antibody ( Cat # PAB3751 ) . Solution : 1 : 1000 diluted in 5% NFM TBS-T 0,05 for overnight ( 15 hrs ) at 4 °C. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham. Rabbit Hu, Ms, Rt E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

ACO2 antibody

Human 721_B; WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. ACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; ACO2 antibody - N-terminal region (ARP56301_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ACO2 antibody

Western Blot: Aconitase 2 Antibody [NBP1-32781] - (30 ug of whole cell lysate) A: 293T B: A431  7.5% SDS PAGE diluted at 1:10000 Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ACO2 antibody

ACO2 Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ACO2 antibody

ACO2 Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ACO2 antibody

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

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