ACO2 antibody

Principal name

ACO2 antibody

Alternative names for ACO2 antibody

Aconitase 2, Aconitate hydratase mitochondrial

SwissProt ID

Q99798 (Human)

Gene ID

50 (ACO2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Ce (C. elegans), Por (Porcine), Can (Canine), Chk (Chicken), Dros (Drosophila), African clawed frog

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of ACO2 antibody

ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Bota DA, Davies KJ. Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism. Nat Cell Biol. 2002 Sep;4(9):674-80.
PMID: 12198491

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACO2 antibody

Western blot analysis of anti-ACO2 (AP11745PU-N) in mouse heart (left) and 293 (right) tissue lysates (35 µg/lane). ACO2 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu, Ms, Rt P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ACO2 (541-555) antibody

  Goat Polyclonal Anti-Aconitase 2 (aa541-555) Antibody  
TA349000 (0.01 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

  anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI3G8 (formerly 3G8)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500824, Dilution 1:50) Mouse IgG1 OTI3G8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ACO2 antibody

  anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7A11 (formerly 7A11)  
Anti-ACO2 mouse monoclonal antibody (TA500841) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307). Mouse IgG1 OTI7A11 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ACO2 antibody

  anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7G4 (formerly 7G4)  
Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500873). Mouse IgG1 OTI7G4 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ACO2 antibody

  Rabbit Polyclonal Antibody against ACO2 (Center)  
Western blot analysis of ACO2 (arrow) using rabbit polyclonal ACO2 Antibody (Center) (Cat.#TA302090).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACO2 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

  Goat Anti-Aconitase 2 / ACO2 Antibody  
(0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

  Anti-ACO2 rabbit polyclonal antibody  
Predicted band size: 85 kDa. Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

  Anti-ACO2 rabbit polyclonal antibody  
Predicted band size: 85 kDa. Positive control: Mouse heart tissue and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Mouse heart tissue lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACO2 antibody

  anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI3G10 (formerly 3G10)  
Anti-ACO2 mouse monoclonal antibody (TA500913) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307). Mouse IgG2a OTI3G10 Purified Hu F, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ACO2 antibody

Human Heart (formalin-fixed, paraffin-embedded) stained with ACO2 antibody AP22773PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ACO2 - human gene knockout kit via CRISPR

ACO2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ACO2 - human gene knockout kit via CRISPR

ACO2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ACO2 - human gene knockout kit via CRISPR

ACO2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ACO2 - human gene knockout kit via CRISPR

ACO2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ACO2

  Recombinant protein of human aconitase 2, mitochondrial (ACO2), nuclear gene encoding mitochondrial protein  
ACO2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ACO2 (mGFP-tagged) - Human aconitase 2, mitochondrial (ACO2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL

Lenti ORF particles, ACO2 (mGFP-tagged) - Human aconitase 2, mitochondrial (ACO2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, ACO2 (Myc-DDK tagged) - Human aconitase 2, mitochondrial (ACO2), nuclear gene encoding mitochondrial protein , 200 uL, >10^7 TU/mL

Lenti ORF particles, ACO2 (Myc-DDK tagged) - Human aconitase 2, mitochondrial (ACO2), nuclear gene encoding mitochondrial protein , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

18 Item(s)

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