ACOT8 antibody

Principal name

ACOT8 antibody

Alternative names for ACOT8 antibody

ACTEIII, PTE1, PTE2, Acyl-CoA thioesterase 8, PTE-2, Choloyl-coenzyme A thioesterase, Peroxisomal acyl-coenzyme A thioester hydrolase 1, Peroxisomal long-chain acyl-CoA thioesterase 1, HIV-Nef-associated acyl-CoA thioesterase, Thioesterase II

SwissProt ID

O14734 (Human), P58137 (Mouse), Q8VHK0 (Rat)

Gene ID

10005 (ACOT8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)

Background of ACOT8 antibody

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A Thioesterase 8, also known as ACOT8 may mediate Nef-induced down-regulation of CD4. It is a major thioesterase in peroxisomes and competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). It shows a preference for medium-length fatty acyl-CoAs and may be involved in the metabolic regulation of peroxisome proliferation.

General readings

Ishizuka M., et al. (2004) Exp. Cell Res. 297:127-141Hunt M.C., et al. (2002) Prog. Lipid Res. 41:99-130

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACOT8 antibody

Western blot analysis of extracts from HUVEC cells, using ACOT8 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACOT8 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ACOT8 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACOT8 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and lung cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ACOT8 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACOT8 (C-term) antibody

Formalin-fixed and paraffin-embedded human Prostate carcinoma reacted with ACOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ACOT8 antibody

Immunofluorescence of purified MaxPab antibody to ACOT8 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ACOT8 antibody

ACOT8 monoclonal antibody (M03), clone 3F1. Western Blot analysis of ACOT8 expression in A-431(Cat # L015V1 ). Mouse IgG1 3F1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ACOT8 antibody

Immunofluorescence analysis of A549 cells, using ACOT8 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

ACOT8 antibody

Immunofluorescence analysis of A549 cells, using ACOT8 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

ACOT8 (N-term) antibody

Human 721_B; WB Suggested Anti-ACOT8 Antibody. . Titration: 1.0 ug/ml. . Positive Control: 721_B Whole Cell. ACOT8 is supported by BioGPS gene expression data to be expressed in 721_B.; ACOT8 antibody - N-terminal region (ARP62050_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ACOT8 antibody

Immunohistochemical analysis of ACOT8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ACOT8 antibody

Immunohistochemical analysis of ACOT8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ACOT8 antibody

Immunohistochemical analysis of ACOT8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ACOT8 antibody

Western Blot: ACOT8 Antibody [NBP1-32452] - (30 ug whole cell lysate) A:MOLT4  B:Raji  10% SDS PAGE diluted at 1:1000 Rabbit IgG Hu WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ACOT8 antibody

ACOT8 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

ACOT8 antibody

ACOT8 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ACOT8 (1-319, His-tag)

ACOT8 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

ACOT8 (1-319, His-tag)

ACOT8 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

ACOT8

ACOT8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ACOT8

ACOT8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ACOT8

ACOT8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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