ACP1 / LMW-PTPase antibody

Principal name

ACP1 / LMW-PTPase antibody

Alternative names for ACP1 / LMW-PTPase antibody

Adipocyte acid phosphatase, Red cell acid phosphatase 1, HAAP, Low molecular weight phosphotyrosine protein phosphatase, Low molecular weight cytosolic acid phosphatase

SwissProt ID

P11064 (Bovin), P24666 (Human), P41498 (Rat), P81693 (Pig), Q5ZKG5 (Chick), Q9D358 (Mouse)

Gene ID

52 (ACP1), 11431 (Acp1), 24161

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), African clawed frog, Can (Canine), GP (Guinea Pig), Gt (Goat), Por (Porcine)

Available hosts

Mouse, Sheep, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ACP1 / LMW-PTPase antibody

Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinylphosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1) 2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.

General readings

Junien C., et al. (1979) Hum Genet. 48(1): 17-21.Dissing J., et al. (1992) Biochim Biophys Acta. 1121: 261-268.Lazaruk KD., et al. (1993) Biochem Biophys Res Commun. 196: 440-446.Bryson GL., et al. (1995) Genomics. 30: 133-140.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACP1 / LMW-PTPase antibody

  Rabbit Polyclonal Anti-ACP1 Antibody  
WB Suggested Anti-ACP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: COLO205 cell lysate; ACP1 is strongly supported by BioGPS gene expression data to be expressed in Human COLO205 cells Rabbit IgG Purified Bov, Can, GP, Gt, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ACP1 / LMW-PTPase antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : Jurkat cell lysate 
Lane 3. : TF-1 cell lysate 
Lane 4. : NIH/3T3 cell lysate
Lane 5. : HepG2 cell lysate Mouse IgG1 AT5C11 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : Jurkat cell lysate 
Lane 3. : TF-1 cell lysate 
Lane 4. : NIH/3T3 cell lysate
Lane 5. : HepG2 cell lysate Mouse IgG1 AT5C11 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase antibody

CHO cells were transfected with either Vector (pSRa) or expression vector driving expression of HCPTPA (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2mg of lysate protein was incubated for 1 h with 10ml of sheep-anti-LMWPTP containing anti-serum, and  immunoprecipitated proteins recovered by Protein A/G were separated on PAGE (12%), and HCPTPA detected using a mouse antisheep monoclonal antibody. Sheep IgG Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase (N-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ACP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Acp1 - mouse gene knockout kit via CRISPR

Acp1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Acp1 - mouse gene knockout kit via CRISPR

Acp1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Acp1 - mouse gene knockout kit via CRISPR

Acp1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Acp1 - mouse gene knockout kit via CRISPR

Acp1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ACP1 / LMW-PTPase (transcript variant 2)

  Recombinant protein of human acid phosphatase 1, soluble (ACP1), transcript variant 2  
ACP1 / LMW-PTPase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ACP1 / LMW-PTPase (transcript variant 3)

  Recombinant protein of human acid phosphatase 1, soluble (ACP1), transcript variant 3  
ACP1 / LMW-PTPase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ACP1 / LMW-PTPase (transcript variant 3)

  Recombinant protein of human acid phosphatase 1, soluble (ACP1), transcript variant 3  
ACP1 / LMW-PTPase Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

ACP1 / LMW-PTPase (1-158, His-tag)

Recombinant human ACP1, 1-158 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase (1-158, His-tag)

Recombinant human ACP1, 1-158 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

ACP1 overexpression lysate

ACP1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ACP1 (mGFP-tagged) - Human acid phosphatase 1, soluble (ACP1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, ACP1 (mGFP-tagged) - Human acid phosphatase 1, soluble (ACP1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ACP1 (mGFP-tagged)-Human acid phosphatase 1, soluble (ACP1), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, ACP1 (mGFP-tagged)-Human acid phosphatase 1, soluble (ACP1), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ACP1 (Myc-DDK tagged) - Human acid phosphatase 1, soluble (ACP1), transcript variant 3 , 200 uL, >10^7 TU/mL

Lenti ORF particles, ACP1 (Myc-DDK tagged) - Human acid phosphatase 1, soluble (ACP1), transcript variant 3 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, ACP1 (Myc-DDK-tagged)-Human acid phosphatase 1, soluble (ACP1), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, ACP1 (Myc-DDK-tagged)-Human acid phosphatase 1, soluble (ACP1), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Acp1 (GFP-tagged) - Mouse acid phosphatase 1, soluble (Acp1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Acp1 (GFP-tagged) - Mouse acid phosphatase 1, soluble (Acp1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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