ACP1 / LMW-PTPase antibody

Principal name

ACP1 / LMW-PTPase antibody

Alternative names for ACP1 / LMW-PTPase antibody

Adipocyte acid phosphatase, Red cell acid phosphatase 1, HAAP, Low molecular weight phosphotyrosine protein phosphatase, Low molecular weight cytosolic acid phosphatase

SwissProt ID

P11064 (Bovin), P24666 (Human), P41498 (Rat), P81693 (Pig), Q5ZKG5 (Chick), Q9D358 (Mouse)

Gene ID

52 (ACP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), African clawed frog

Available hosts

Mouse, Sheep, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Paraffin Sections (P), Functional assay (FN), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ACP1 / LMW-PTPase antibody

Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinylphosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1) 2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.

General readings

Junien C., et al. (1979) Hum Genet. 48(1): 17-21.Dissing J., et al. (1992) Biochim Biophys Acta. 1121: 261-268.Lazaruk KD., et al. (1993) Biochem Biophys Res Commun. 196: 440-446.Bryson GL., et al. (1995) Genomics. 30: 133-140.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACP1 / LMW-PTPase antibody

Western Blot analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody (M01), clone 4B10.

Lane 1: ACP1 transfected lysate(18 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 4B10 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ACP1 / LMW-PTPase antibody

Western Blot analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody (M06), clone 2A3.

Lane 1: ACP1 transfected lysate(18 KDa).
Lane 2: Non-transfected lysate. Mouse IgG 2A3 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ACP1 / LMW-PTPase antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : Jurkat cell lysate 
Lane 3. : TF-1 cell lysate 
Lane 4. : NIH/3T3 cell lysate
Lane 5. : HepG2 cell lysate Mouse IgG1 AT5C11 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : Jurkat cell lysate 
Lane 3. : TF-1 cell lysate 
Lane 4. : NIH/3T3 cell lysate
Lane 5. : HepG2 cell lysate Mouse IgG1 AT5C11 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase antibody

CHO cells were transfected with either Vector (pSRa) or expression vector driving expression of HCPTPA (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2mg of lysate protein was incubated for 1 h with 10ml of sheep-anti-LMWPTP containing anti-serum, and  immunoprecipitated proteins recovered by Protein A/G were separated on PAGE (12%), and HCPTPA detected using a mouse antisheep monoclonal antibody. Sheep IgG Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase (N-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ACP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase antibody

Western Blot analysis of ACP1 expression in transfected 293T cell line (H00000052-T01) by ACP1 MaxPab polyclonal antibody.

Lane 1: ACP1 transfected lysate(18.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ACP1 / LMW-PTPase antibody

ACP1 / LMW-PTPase Mouse IgG2a 2A15 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ACP1 / LMW-PTPase antibody

Western blot analysis of ACP1 in the 1 ) absence and 2 ) presence of immunizing peptide in human liver lysate. Using ACP1 polyclonal antibody ( Cat # PAB0152 ) at 1 µg/ml. Rabbit Bov, Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ACP1 / LMW-PTPase antibody

Western blot analysis of ACP1 in the 1 ) absence and 2 ) presence of immunizing peptide in human liver lysate. Using ACP1 polyclonal antibody ( Cat # PAB0285 ) at 1 µg/ml. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ACP1 / LMW-PTPase antibody

human Kidney; Rabbit Anti-ACP1 Antibody. Catalog Number: ARP42178. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; ACP1 antibody - N-terminal region (ARP42178_T100) in human Kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

ACP1 / LMW-PTPase antibody

Human COLO205; WB Suggested Anti-ACP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate. ACP1 is strongly supported by BioGPS gene expression data to be expressed in Human COLO205 cells.; ACP1 antibody - middle region (ARP42343_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ACP1 / LMW-PTPase antibody

ACP1 / LMW-PTPase Rabbit IgG Hu WB
0.1 ml / €340.00
  Novus Biologicals Inc.

ACP1 / LMW-PTPase antibody

Western Blot: ACP1 Antibody [NBP1-55487] - This Anti-ACP1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1.25ug/ml. Rabbit Purified Hu P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

ACP1 / LMW-PTPase antibody

Immunohistochemistry-Paraffin: ACP1 Antibody [NBP1-89551] -  staining of human testis shows cytoplasmic positivity of cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ACP1 / LMW-PTPase (transcript variant 2)

ACP1 / LMW-PTPase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ACP1 / LMW-PTPase (transcript variant 3)

ACP1 / LMW-PTPase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ACP1 / LMW-PTPase (transcript variant 3)

ACP1 / LMW-PTPase Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

ACP1 / LMW-PTPase (1-158, His-tag)

Recombinant human ACP1, 1-158 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

ACP1 / LMW-PTPase (1-158, His-tag)

Recombinant human ACP1, 1-158 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

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